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ID   PAD3       preliminary; circular DNA; SYN; 2681 BP.
XX
AC   IG9865;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pAD3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAD3 from pUC9
RA   Schaller H.;
RT   ;
RL   Unpublished (1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pAD3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli DH1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC9 2665bp, 3' to lacZ
FT                   2. linker EcoRI-BamHI 12bp gaattcggatcc
FT                   -> pAD3 2677bp [BamHI-EcoRI-lacZ-EcoRI-BamHI]"
FT   -               1..428
FT                   /note="pUC9 1..428 428bp
FT                   HaeII = RGCGC^Y
FT                   \             gaattcggatcc"
FT   -               429..440
FT                   /note="gaattcggatcc 12bp
FT                   \ gaattcggatcc
FT                   HaeII =      R GCGC^Y"
FT   -               441..2681
FT                   /note="pUC9 425..2665 2241bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
FT   CDS             0..0
FT                   /note="ANT E. coli ampicillin resistance gene (amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 2681 BP; 657 A; 684 C; 676 G; 664 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatcccc gggaattcac tggccgtcgt tttacaacgt cgtgactggg
     aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     aatggcgcga attcggatcc gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//