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ID   PADBETA    preliminary; circular DNA; SYN; 7069 BP.
XX
AC   IG1024; ATCC77179;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pADbeta - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pADbeta from adenovirus 2 MLP & SV40 splice/polyA & pUC19
RC   pCMVbeta from CMV IEP & SV40 splice/polyA & pUC19
RC   pSVbeta from SV40 early promoter & SV40 splice/polyA & pUC19
RC   pTKbeta from HSV TK gene promoter & SV40 splice/polyA & pUC19
RA   MacGregor G.R., Caskey C.T.;
RT   "Construction of plasmids that express E. coli beta-galactosidase
RT   in mammalian cells";
RL   Nucleic Acids Res. 17:2365-2365(1989).
XX
RN   [2]
RC   pZ-1 from pBR322 & pMC1871
RC   pPN-7 from pZ-1 & pATV-8
RC   pPN-1 from pMC1403 & pATV-8
RC   pPN-2 from pPN-1 & pATV-8
RC   pPN-10 from pRAV-1
RC   pPN-11 from pRAV-1
RC   pPN-12 from pRAV-1
RA   Norton P.A., Coffin J.M.;
RT   "Bacterial beta-galactosidase as a marker of Rous sarcoma virus
RT   gene expression and replication";
RL   Mol. Cell. Biol. 5:281-290(1985).
XX
RN   [3]
RC   pSV0cat, pSV2cat, pA10CAT2, pCAT..., pBLCAT2, pBLCAT3 from cat gene
RC   pSK-SVCAT, pSK-CAT from cat gene
RC   pRSVL-Adelta5', pSV2AL-Adelta5', pT81luc, pSluc2 from luciferase gene
RC   pXP1, pXP2, pOLUC from luciferase gene
RC   pXGH, pTKGH, pOGH from growth hormone gene
RC   pSV2Apap, pSV232Apap, pSV0Apap from alkaline phosphatase gene
RC   pBC12/RSV/SEAP, pBC12/PL/SEAP from secreted alkaline phosphatase
RC   pCMVbeta, pSVbeta, pNASSbeta from beta-galactosidase gene
RA   Alam J., Cook J.L.;
RT   "Reporter genes: application to the study of mammalian gene
RT   transcription";
RL   Anal. Biochem. 188:245-254(1990).
XX
RN   [4]
RC   pATV-6, pATV-7, pATV-8, pATV-9 from pBR322 & ASV Prague C, ASV LTR
RA   Katz R.A., Omer C.A., Weis J.H., Mitsialis S.A., Faras A.J.,
RA   Guntaka R.V.;
RT   "Restriction endonuclease and nucleotide sequence analysis of
RT   molecularly cloned unintegrated avian tumor virus DNA: structure
RT   of large terminal repeats in circle junctions";
RL   J. Virol. 42:346-351(1982).
XX
RN   [5]
RC   AEV from chicken
RC   RAV-1 from chicken
RC   pAEV11-3 from pBR313 & AEV
RC   pRAV-1, pRAV10-R from pBR322 & RAV-1
RA   Sealy L., Privalsky M.L., Moscovici G., Moscovici C., Bishop J.M.;
RT   "Site-specific mutagenesis of avian erythrobalastosis virus: erb-B
RT   is required for oncogenicity";
RL   Virology 130:155-178(1983).
XX
RN   [6]
RC   RAV-1 from chicken
RC   RAV-2 from chicken
RC   lambda SRB-td7, lambda SRB-td8 from lambda & RAV-2
RA   Ju G., Skalka A.M.;
RT   "Nucleotide sequence analysis of the long terminal repeat (LTR) of
RT   avian retroviruses: structural similarities with transposable
RT   elements";
RL   Cell 22:379-386(1980).
XX
CC   ATCC size is 7200 bp.
CC   The order of the major features in this plasmid is: pUC19 - EcoRI -
CC   adenovirus 2 major late promoter/tripartite leader/splice signal -
CC   NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI -
CC   HindIII - pUC19. [1]
CC   The lacZ gene may be excised by NotI and replaced with alternative
CC   sequences. [1]
CC   Reporter plasmid permitting visual detection of beta-galactosidase
CC   activity by histochemical staining. [1]
CC   Designed primarily as an enhancer trap vector.  May be used as a
CC   reference plasmid in transfection experiments or to monitor the
CC   effect of trans-acting factors. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6;
CC   NotI--3.6 (doublet); BamHI--7.0, 0.2. (ATCC staff)
CC   Medium is -1 TB plus ampicillin (200 ug/ml).
CC   NM (pADbeta)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(ATCC)
CC   HO (E.coli DH5alpha)(E.coli)(monkey COS cells)(vertebrate cells)
CC   CP ()
CC   FN (expression)(reporter)(promoter analysis)
CC   SE ()
CC   PA (pUC19)
CC   BR (pNASSbeta)(pCMVbeta)(pSVbeta)(pTKbeta)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. SV40, splice/polyA
FT                   -> plasmid
FT                   1. plasmid NotI
FT                   2. E. coli NotI-NotI, lacZ gene
FT                   -> plasmid2
FT                   1. plasmid2
FT                   2. adenovirus 2, MLP
FT                   -> pADbeta 7069bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   promoter        0..0
FT                   /note="PRO adenovirus 2 major late gene and
FT                   tripartite leader"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-BamHI-XbaI-SalI-PstI-SphI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    3900..3900
FT                   /note="SIT HindIII"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 7069 BP; 1645 A; 1828 C; 1915 G; 1681 T; 0 other;
     gaattcgagc tcggtactcg agcggtgttc cgcggtcctc ctcgtataga aactcggacc
     actctgagac gaaggctcgc gtccaggcca gcacgaagga ggctaagtgg gaggggtagc
     ggtcgttgtc cactaggggg tccactcgct ccagggtgtg aagacacatg tcgccctctt
     cggcatcaag gaaggtgatt ggtttatagg tgtaggccac gtgaccgggt gttcctgaag
     gggggctata aaagggggtg ggggcgcgtt cgtcctcact ctcttccgca tcgctgtctg
     cgagggccag ctgttgggct cgcggttgag gacaaactct tcgcggtctt tccagtactc
     ttggatcgga aacccgtcgg cctccgaacg gtactccgcc accgagggac ctgagcgagt
     ccgcatcgac cggatcggaa aacctctcga ctgttggggt gagtactccc tctcaaaagc
     gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt
     tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat
     gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaacc ggaattgtac
     ccgcggccgc aattcccggg gatcgaaaga gcctgctaaa gcaaaaaaga agtcaccatg
     tcgtttactt tgaccaacaa gaacgtgatt ttcgttgccg gtctgggagg cattggtctg
     gacaccagca aggagctgct caagcgcgat cccgtcgttt tacaacgtcg tgactgggaa
     aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
     cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
     cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
     aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
     cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
     tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
     cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg
     gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa
     ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
     gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
     gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
     attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg
     ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     tcctggagcc cgtcagtatc ggcggaatta cagctgagcg ccggtcgcta ccattaccag
     ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     aggaaatcca ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt
     ttcttttact tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac
     atcaaccata tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg
     ctattattcc aaccgctgtt tggtctgctt tctgacaaac tcggcctcga ctctaggcgg
     ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     gggggaggtg tgggaggttt tttcggatcc tctagagtcg acctgcaggc atgcaagctt
     ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagt
//