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ID   PALTER1    preliminary; circular DNA; SYN; 5680 BP.
XX
AC   X65334; IG0152; ATCC68196;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pALTER-1 (formerly pSELECT-1) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5680
RC   pALTER-1, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-5680
RC   pALTER-1
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [3]
RC   from pBR322 & human SAP-1 gene
RA   Dewji N., Wenger D., Fujibayashi S., Donoviel M., Esch F., Hill F.,
RA   O'Brien J.S.;
RT   "Molecular cloning of the sphingolipid activator protein-1 (SAP-1),
RT   the sulfatide sulfatase activator";
RL   Biochem. Biophys. Res. Commun. 134:989-994(1986).
XX
CC   pSELECT-1 had 5675 bp reported.
CC   NM (pALTER-1)(pSELECT-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)(ATCC)
CC   HO (E.coli JM109)(E.coli)
CC   CP ()
CC   FN (mutagenesis)(sequencing)(transcription)
CC   SE (color blue/white)
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene/tet gene
FT                   2. T7, promoter
FT                   3. Sp6, promoter
FT                   4. E. coli, lac promoter/lacZ gene
FT                   5. phage f1, ori
FT                   -> pALTER-1 5680bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    5..61
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT                   SalI-PstI-SphI-HindIII"
FT   misc_feature    69..69
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        64..86
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    94..323
FT                   /note="E. coli lac operon"
FT   primer_bind     104..120
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    complement(108..108)
FT                   /note="E. coli lacZ start codon"
FT   promoter        128..144
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3750..4606)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      5045..5500
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_feature    5501..5661
FT                   /note="E. coli lac operon"
FT   primer_bind     5621..5637
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(5654..5676,1..6)
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 5680 BP; 1321 A; 1525 C; 1511 G; 1323 T; 0 other;
     gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatga attaattctc atgtttgaca gcttatcatc
     gattagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt
     atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac
     gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat
     gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     atgcaggagt cgcataaggg agagcgccga ccgatgccct tgagagcctt caacccagtc
     agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa
     catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttt ggctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgcggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgaaattg taaacgttaa tattttgtta aaattcgcgt taaatatttg ttaaatcagc
     tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg
     agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//