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ID PALTER1 preliminary; circular DNA; SYN; 5680 BP.
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AC X65334; IG0152; ATCC68196;
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DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pALTER-1 (formerly pSELECT-1) - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5680
RC pALTER-1, revised by [2]
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-5680
RC pALTER-1
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [3]
RC from pBR322 & human SAP-1 gene
RA Dewji N., Wenger D., Fujibayashi S., Donoviel M., Esch F., Hill F.,
RA O'Brien J.S.;
RT "Molecular cloning of the sphingolipid activator protein-1 (SAP-1),
RT the sulfatide sulfatase activator";
RL Biochem. Biophys. Res. Commun. 134:989-994(1986).
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CC pSELECT-1 had 5675 bp reported.
CC NM (pALTER-1)(pSELECT-1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Promega)(ATCC)
CC HO (E.coli JM109)(E.coli)
CC CP ()
CC FN (mutagenesis)(sequencing)(transcription)
CC SE (color blue/white)
CC PA (pBR322)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/amp gene/tet gene
FT 2. T7, promoter
FT 3. Sp6, promoter
FT 4. E. coli, lac promoter/lacZ gene
FT 5. phage f1, ori
FT -> pALTER-1 5680bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 5..61
FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-
FT SalI-PstI-SphI-HindIII"
FT misc_feature 69..69
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter 64..86
FT /note="PRO bacteriophage Sp6"
FT misc_feature 94..323
FT /note="E. coli lac operon"
FT primer_bind 104..120
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature complement(108..108)
FT /note="E. coli lacZ start codon"
FT promoter 128..144
FT /note="PRO E. coli lac operator"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(3750..4606)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 5045..5500
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_feature 5501..5661
FT /note="E. coli lac operon"
FT primer_bind 5621..5637
FT /note="PRI bacteriophage M13 forward"
FT promoter join(5654..5676,1..6)
FT /note="PRO bacteriophage T7"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ Sequence 5680 BP; 1321 A; 1525 C; 1511 G; 1323 T; 0 other;
gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
aatcagggga taacgcagga aagaacatga attaattctc atgtttgaca gcttatcatc
gattagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt
atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac
gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat
gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
atgcaggagt cgcataaggg agagcgccga ccgatgccct tgagagcctt caacccagtc
agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa
catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
agtatatatg agtaaacttt ggctgacagt taccaatgct taatcagtga ggcacctatc
tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgcggcatc gtggtgtcac
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
ggcgaaattg taaacgttaa tattttgtta aaattcgcgt taaatatttg ttaaatcagc
tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg
agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//