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ID PAMP2 preliminary; circular DNA; SYN; 4114 BP.
XX
AC IG9003;
XX
DT 01-FEB-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pAMP2 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo]
RC [pAMP18 from pUC18 & oligo]
RC [pAMP19 from pUC19 & oligo]
RA Rashtchian A., Thorton C.G., Heidecker G.;
RT "A novel method for site-directed mutagenesis using PCR and uracil
RT DNA glycosylase";
RL PCR Meth. Appl. 2:124-130(1992).
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RN [2]
RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo]
RC [pAMP18 from pUC18 & oligo]
RC [pAMP19 from pUC19 & oligo]
RA Higuchi R., Krummel B., Saiki R.K.;
RT "A general method of in vitro preparation and specific mutagenesis
RT of DNA fragments: study of protein and DNA interactions";
RL Nucleic Acids Res. 16:7351-7367(1988).
XX
RN [3]
RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo]
RC [pAMP18 from pUC18 & oligo]
RC [pAMP19 from pUC19 & oligo]
RA Owen J.L., Hay C., Schuster D.M., Rashtchian A.;
RT ;
RL Focus 16:39-39(1994).
XX
RN [4]
RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo]
RC [pAMP18 from pUC18 & oligo]
RC [pAMP19 from pUC19 & oligo]
RA Rashtchian A., Buchman G.W., Schuster D.M., Berninger M.S.;
RT "Uracil DNA glycosylase-mediated cloning of polymerase chain
RT reaction-amplified DNA: application to genomic and cDNA cloning";
RL Anal. Biochem. 206:91-97(1992).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pAMP2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (BRL)
CC HO (E.coli)
CC CP (high)
CC FN (cloning)(expression)(transcription)
CC SE ()
CC PA (pSPORT1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSPORT1 remove NotI-SalI 21bp 233..254,
FT \ MCS 4088bp
FT 2. oligo NotI-XmaIII-SalI 30bp
FT \ ggccgcctactactactaatgatgatgatg
FT -> pAMP2 4118bp"
FT - 1..232
FT /note="pSPORT1 1..232 232bp
FT NotI = GC^GGCCGC
FT \ catcatcatcattagtagtagtaggcggcc"
FT - 233..262
FT /note="catcatcatcattagtagtagtaggcggcc 30bp
FT \ catcatcatcattagtagtagtaggcggcc
FT SalI = G^TCGA C"
FT - 263..4114
FT /note="pSPORT1 258..4109 3852bp"
FT CDS complement(0..0)
FT /note="REP E. coli lacZ gene"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT misc_binding 125..381
FT /note="MCS AatII-SphI-MluI-SunI-SnaBI-HindIII-BamHI-
FT XbaI-NotI-XmaIII-SalI/AccI-SmaI-EcoRI-Kpn2I-RsrII-
FT PinAI-KpnI-Sse8387I-PstI"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT CDS complement(0..0)
FT /note="REP E. coli lacI repressor gene"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
XX
SQ Sequence 4114 BP; 1006 A; 1063 C; 1060 G; 985 T; 0 other;
cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gccatcatca
tcattagtag tagtaggcgg cccccgggaa ttccggaccg gtacctgcag gcgtaccagc
tttccctata gtgagtcgta ttagagcttg gcgtaatcat ggtcatagct gtttcctgtg
tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc
ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacccg
ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct ctgcattaat
gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
ccgccgcgct taatgcgccg ctacagggcg cgtc
//