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ID   PAN26      preliminary; circular DNA; SYN; 7447 BP.
XX
AC   U09715;
XX
DT   22-JUN-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Filamentous fungi cosmid vector pAN26 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pAN26 from pTZ19R & lambda, cos
RA   Taylor J.L., Borgmann I.E.;
RT   "A deluxe cosmid vector for transformation of most filamentous fungi";
RL   Unpublished (1994).
XX
RN   [2]
RC   pAN26 from pTZ19R & lambda, cos
RC   pAN52-1 from A.nidulans gpdA gene
RA   Punt P.J., Oliver R.P., Dingemanse M.A., Pouwels P.H.,
RA   Van Den Hondel C.A.;
RT   "Transformation of Aspergillus based on the hygromycin B resistance
RT   marker from Escherichia coli";
RL   Gene 56:117-124(1987).
XX
RN   [3]
RP   1-7447
RC   pAN26
RA   Taylor J.L.;
RT   ;
RL   Submitted (13-MAY-1994) by:
RL   Taylor J.L., NRC Plant Biotechnology Institute, 110 Gymnasium Place,
RL   Saskatoon, Saskatchewan, S7N 0W9, CANADA.
XX
CC   NCBI gi: 501070
CC   NM (pAN26)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ19R 2863bp
FT                   2. Aspergillus nidulans, gpd promoter
FT                   3. E. coli 1142bp, hyg gene
FT                   4. Aspergillus nidulans, trpC gene terminator
FT                   5. lambda, cos
FT                   -> pAN26 7447bp"
FT   promoter        177..194
FT                   /note="PRO T3 promoter"
FT   promoter        complement(232..252)
FT                   /function="PRO SP6 promoter"
FT   promoter        266..2432
FT                   /note="PRO Aspergillus nidulans gene (gpd gene)"
FT   CDS             2429..3448
FT                   /note="GEN hygromycin phosphotransferase gene
FT   terminator      3449..4013
FT                   /note="TER Aspergillus nidulans trpC gene"
FT   misc_signal     4013..4407
FT                   /note="lambda packaging site"
FT   misc_signal     4407..4788
FT                   /note="lambda packaging site"
FT   promoter        complement(4797..4814)
FT                   /function="PRO T7 promoter"
XX
SQ   Sequence 7447 BP; 1827 A; 1896 C; 1828 G; 1896 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtaccaatt
     aaccctcact aaagggagat ctggccgact tggccggatc cgcggccgcg gttctatagt
     gtcacctaaa tggtacccgg ggatctttcg acactgaaat acgtcgagcc tgctccgctt
     ggaagcggtg aggagcctcg tcctgtcaca actaccaaca tggagtacga taagggccag
     ttccgccagc tcattaagag ccagttcatg ggcgttggca tgatggccgt catgcatctg
     tacttcaagt acaccaaccc tcttctgatc cagtcgatca tcccgctgaa gggcgctttc
     gaatcgaatc tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt
     ccctttaagg ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc
     ctccagaacg ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga
     agtcggagga cggagcggtg tcaagaggat attcttcgct ctgtattata gataagatga
     tgaggaattg gaggtagcat agcttcattt ggatttgctt tccaggctga gactctagct
     tggagcatag agggtccctt tggctttcaa tattctcaag tatctcgagt ttgaacttat
     tcccgtgaac cttttattca ccaatgagca ttggaatgaa catgaatctg aggactgcaa
     tcgccatgag gttttcgaaa tacatccgga tgtcgaaggc ttggggcacc tgcgttggtt
     gaatttagaa cgtggcacta ttgatcatcc gatagctctc caaagggcgt tgcacaatgc
     aagtcaaacg ttgctagcag ttccaggtgg aatgttatga tgagcattgt attaaatcag
     gagatatagc atgatctcta gttagctcac cacaaagtca gagcgctaac caaaagtcac
     acaacacaag ctgtaaggat ttcggcacgg ctacggaaga cggagaagcc accttcagtg
     gactcgagta ccatttaatt ctatttgtgt ttgatcgaga cctaatacag cccctacaac
     gaccatcaaa gtcgtatagc taccagtgag gaagtggact caaatcgact tcagcaacat
     ctcctggata aactttaagc ctaaactata cagaataaga taggtggaga gcttataccg
     agctcccaaa tctgtccaga tcatggttga ccggtgcctg gatcttccta tagaatcatc
     cttattcgtt gacctagctg attctggagt gacccagagg gtcatgactt gagcctaaaa
     tccgccgcct ccaccatttg tagaaaaatg tgacgaactc gtgagctctg tacagtgacc
     ggtgactctt tctggcatgc ggagagacgg acggacgcag agagaagggc tgagtaataa
     gccactggcc agacagctct ggcggctctg aggtgcagtg gatgattatt aatccgggac
     cggccgcccc tccgccccga agtggaaagg ctggtgtgcc cctcgttgac caagaatcta
     ttgcatcatc ggagaatatg gagcttcatc gaatcaccgg cagtaagcga aggagaatgt
     gaagccaggg gtgtatagcc gtcggcgaaa tagcatgcca ttaacctagg tacagaagtc
     caattgcttc cgatctggta aaagattcac gagatagtac cttctccgaa gtaggtagag
     cgagtacccg gcgcgtaagc tccctaattg gcccatccgg catctgtagg gcgtccaaat
     atcgtgcctc tcctgctttg cccggtgtat gaaaccggaa aggccgctca ggagctggcc
     agcggcgcag accgggaaca caagctggca gtcgacccat ccggtgctct gcactcgacc
     tgctgaggtc cctcagtccc tggtaggcag ctttgccccg tctgtccgcc cggtgtgtcg
     gcggggttga caaggtcgtt gcgtcagtcc aacatttgtt gccatatttt cctgctctcc
     ccaccagctg ctcttttctt ttctctttct tttcccatct tcagtatatt catcttccca
     tccaagaacc tttatttccc ctaagtaagt actttgctac atccatactc catccttccc
     atcccttatt cctttgaacc tttcagttcg agctttccca cttcatcgca gcttgactaa
     cagctacccc gcttgagcag acatcacaat gcctgaactc accgcgacgt ctgtcgagaa
     gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     cagcactcgt ccgagggcaa aggaatagtg atttaatagc tccatgtcaa caagaataaa
     acgcgttttc gggtttacct cttccagata cagctcatct gcaatgcatt aatgcattga
     ctgcaaccta gtaacgcctt caggctccgg cgaagagaag aatagcttag cagagctatt
     ttcattttcg ggagacgaga tcaagcagat caacggtcgt caagagacct acgagactga
     ggaatccgct cttggctcca cgcgactata tatttgtctc taattgtact ttgacatgct
     cctcttcttt actctgatag cttgactatg aaaattccgt caccagccct gggttcgcaa
     agataattgc atgtttcttc cttgaactct caagcctaca ggacacacat tcatcgtagg
     tataaacctc gaaatcattc ctactaagat ggtatacaat agtaaccatg gttgcctagt
     gaatgctccg taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg
     tttacccaga atgcacaggt acacttgttt agaggtaatc cttctttcta gacgatacct
     gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga
     tgataatcat tatcacttta cgggtccttt ccggtgatcc gacaggttac ggggcggcga
     cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc
     gtcataactt aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct
     gaaagcgagc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca
     atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg taccattcag
     aactggcagg aacagatcga taagcttgtt cctgccagtt ctgaatggta cggatactcg
     caccgaaaat gtcagccagc tgctttttgt tgacttccat tgttcattcc acggacaaaa
     acagagaaag gaaacgacag aggccaaaaa gctcgctttc agcacctgtc gtttcctttc
     ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac ggaaacgcct
     taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc
     ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc acaacgtgcg
     tggaggccat caaaccacgt caaataatca attatgacgc aggtatcagc tttccctata
     gtgagtcgta ttaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
     ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacaaaat attaacgttt
     acaattt
//