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ID PAN521N preliminary; circular DNA; SYN; 5721 BP.
XX
AC Z32697;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAN52-1N - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 999-2572
RC M13mAN5-7B from M13mp11 & gpdA gene
RC from pAN5-41B
RC from pAN923-41B
RC from pAN923-42B
RA Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT "Functional elements in the promoter region of the Aspergillus
RT nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT dehydrogenase";
RL Gene 93:101-109(1990).
XX
RN [2]
RP 1-5721
RC pAN series from gpdA gene
RA Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA Van Den Hondel C.A.M.J.J.;
RT "Vectors for efficient production of homologous and heterologous
RT proteins in filamentous fungi";
RL Unpublished (1994).
XX
RN [3]
RP 1-5721
RC pAN52-1N
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
XX
CC NCBI gi: 475161
CC NM (pAN52-1N)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN52-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 EcoRI-SalI 2653bp 451..2686..418
FT 2. A.nidulans SalI-EcoRI 3070bp, gpdA gene
FT -> pAN52-1N 5721bp"
FT promoter 1..2129
FT /note="PRO A. nidulans gpdA gene;
FT EXPERIMENTAL [1],[2]"
FT misc_feature 2130..2130
FT /note="A. nidulans gpdA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT intron 2178..2293
FT /note="EXPERIMENTAL [1]"
FT terminator 2305..3071
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 2565..2575
FT /note="replace(2565..2575,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2774..2782
FT /note="replace(2774..2782,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 2862..2872
FT /note="replace(2862..2872,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 2899..2906
FT /note="replace(2899..2906,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 2555..2565
FT /note="partial"
FT polyA_site 2828..2838
FT /note="partial"
FT misc_feature 3072..5721
FT /note="from pUC18 SalI-EcoRI [2]"
FT misc_feature 1..5721
FT /note="fungal expression vector pAN52-1;
FT EXPERIMENTAL [2]"
FT misc_feature 2356..2375
FT /note="PRO_PEPTIDE sequence for putative propeptide;
FT contains dibasic processing site (LYS-ARG) [2]"
FT CDS 2302..2457
FT /note="gpdA secretion signal sequence very similar to
FT that of the A.niger glucoamylase gene;
FT NCBI gi: 475898; EXPERIMENTAL [2]"
XX
SQ Sequence 5721 BP; 1433 A; 1450 C; 1374 G; 1461 T; 3 other;
gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
cccgcttgag cagacatcac catggatcca cttaacgtta ctgaaatcat caaacagctt
gacgaatctg gatataagat cgttggtgtc gatgtcagct ccggagttga gacaaatggt
gttcaggatc tcgataagat acgttcattt gtccaagcag caaagagtgc cttctagtga
tttaatagct ccatgtcaac aagaataaaa cgcgttttcg ggtttacctc ttccagatac
agctcatctg caatgcatta atgcattgac tgcaacctag taacgccttn caggctccgg
cgaagagaag aatagcttag cagagctatt ttcattttcg ggagacgaga tcaagcagat
caacggtcgt caagagacct acgagactga ggaatccgct cttggctcca cgcgactata
tatttgtctc taattgtact ttgacatgct cctcttcttt actctgatag cttgactatg
aaaattccgt caccagcncc tgggttcgca aagataattg catgtttctt ccttgaactc
tcaagcctac aggacacaca ttcatcgtag gtataaacct cgaaatcant tcctactaag
atggtataca atagtaacca tgcatggttg cctagtgaat gctccgtaac acccaatacg
ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc acaggtacac
ttgtttagag gtaatccttc tttctagaag tcctcgtgta ctgtgtaagc gcccactcca
catctccact cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
aacagctatg accatgatta c
//