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ID   PAN521N    preliminary; circular DNA; SYN; 5721 BP.
XX
AC   Z32697;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAN52-1N - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   999-2572
RC   M13mAN5-7B from M13mp11 & gpdA gene
RC   from pAN5-41B
RC   from pAN923-41B
RC   from pAN923-42B
RA   Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA   Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT   "Functional elements in the promoter region of the Aspergillus
RT   nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT   dehydrogenase";
RL   Gene 93:101-109(1990).
XX
RN   [2]
RP   1-5721
RC   pAN series from gpdA gene
RA   Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA   Van Den Hondel C.A.M.J.J.;
RT   "Vectors for efficient production of homologous and heterologous
RT   proteins in filamentous fungi";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-5721
RC   pAN52-1N
RA   Punt P.;
RT   ;
RL   Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL   Rijkswik, The Netherlands.
XX
CC   NCBI gi: 475161
CC   NM (pAN52-1N)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAN52-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 EcoRI-SalI 2653bp 451..2686..418
FT                   2. A.nidulans SalI-EcoRI 3070bp, gpdA gene
FT                   -> pAN52-1N 5721bp"
FT   promoter        1..2129
FT                   /note="PRO A. nidulans gpdA gene;
FT                   EXPERIMENTAL [1],[2]"
FT   misc_feature    2130..2130
FT                   /note="A. nidulans gpdA gene major
FT                   transcription start site; EXPERIMENTAL [1]"
FT   intron          2178..2293
FT                   /note="EXPERIMENTAL [1]"
FT   terminator      2305..3071
FT                   /note="TER A. nidulans trpC gene terminator region
FT                   (containing CDS); EXPERIMENTAL [2],[3];
FT                   contains the 3'end of the trpC
FT                   transcript including 50 codons; two major
FT                   transcription ends have been identified"
FT   misc_difference 2565..2575
FT                   /note="replace(2565..2575,gccttncaggc);
FT                   introduction of one base at this position generates
FT                   an BlgI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 2774..2782
FT                   /note="replace(2774..2782,cagcncctg);
FT                   introduction of one base at this position generates
FT                   an AlwNI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 2862..2872
FT                   /note="replace(2862..2872,gaaatcanttc);
FT                   introduction (actual sequence could be deletion as
FT                   well) of a base at this position results in the
FT                   elimination of an XmnI site which was shown to be
FT                   absent by restriction digestion [3]"
FT   mutation        2899..2906
FT                   /note="replace(2899..2906,catgcatg);
FT                   A NcoI site present at this position was removed by
FT                   digestion, treatment with klenow polymerase and
FT                   religation [2]"
FT   polyA_site      2555..2565
FT                   /note="partial"
FT   polyA_site      2828..2838
FT                   /note="partial"
FT   misc_feature    3072..5721
FT                   /note="from pUC18 SalI-EcoRI [2]"
FT   misc_feature    1..5721
FT                   /note="fungal expression vector pAN52-1;
FT                   EXPERIMENTAL [2]"
FT   misc_feature    2356..2375
FT                   /note="PRO_PEPTIDE sequence for putative propeptide;
FT                   contains dibasic processing site (LYS-ARG) [2]"
FT   CDS             2302..2457
FT                   /note="gpdA secretion signal sequence very similar to
FT                   that of the A.niger glucoamylase gene;
FT                   NCBI gi: 475898; EXPERIMENTAL [2]"
XX
SQ   Sequence 5721 BP; 1433 A; 1450 C; 1374 G; 1461 T; 3 other;
     gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
     cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
     cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
     agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
     cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
     ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
     tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
     ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
     ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
     cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
     ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
     gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
     ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
     ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
     acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
     gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
     catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     cccgcttgag cagacatcac catggatcca cttaacgtta ctgaaatcat caaacagctt
     gacgaatctg gatataagat cgttggtgtc gatgtcagct ccggagttga gacaaatggt
     gttcaggatc tcgataagat acgttcattt gtccaagcag caaagagtgc cttctagtga
     tttaatagct ccatgtcaac aagaataaaa cgcgttttcg ggtttacctc ttccagatac
     agctcatctg caatgcatta atgcattgac tgcaacctag taacgccttn caggctccgg
     cgaagagaag aatagcttag cagagctatt ttcattttcg ggagacgaga tcaagcagat
     caacggtcgt caagagacct acgagactga ggaatccgct cttggctcca cgcgactata
     tatttgtctc taattgtact ttgacatgct cctcttcttt actctgatag cttgactatg
     aaaattccgt caccagcncc tgggttcgca aagataattg catgtttctt ccttgaactc
     tcaagcctac aggacacaca ttcatcgtag gtataaacct cgaaatcant tcctactaag
     atggtataca atagtaacca tgcatggttg cctagtgaat gctccgtaac acccaatacg
     ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc acaggtacac
     ttgtttagag gtaatccttc tttctagaag tcctcgtgta ctgtgtaagc gcccactcca
     catctccact cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
     gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     aacagctatg accatgatta c
//