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ID   PAN561H    preliminary; circular DNA; SYN; 7564 BP.
XX
AC   Z32700;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAN56-1H - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   999-2572
RC   M13mAN5-7B from M13mp11 & gpdA gene
RC   from pAN5-41B
RC   from pAN923-41B
RC   from pAN923-42B
RA   Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA   Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT   "Functional elements in the promoter region of the Aspergillus
RT   nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT   dehydrogenase";
RL   Gene 93:101-109(1990).
XX
RN   [2]
RP   1-7564
RC   pAN series from gpdA gene
RA   Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA   Van Den Hondel C.A.M.J.J.;
RT   "Vectors for efficient production of homologous and heterologous
RT   proteins in filamentous fungi";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-7564
RC   pAN56-1H
RA   Punt P.;
RT   ;
RL   Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL   Rijkswik, The Netherlands.
XX
CC   NCBI gi: 475165
CC   NM (pAN56-1H)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAN56-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 EcoRI-HindIII 2635bp 451..2686..400
FT                   2. A.nidulans HindIII-EcoRI 4930bp, gpdA gene
FT                   -> pAN56-1H 7564bp"
FT   promoter        1..2133
FT                   /note="PRO A. nidulans gpdA gene;
FT                   EXPERIMENTAL [1],[2]"
FT   misc_feature    2134..2134
FT                   /note="A. nidulans gpdA gene major
FT                   transcription start site; EXPERIMENTAL [1]"
FT   intron          2182..2297
FT                   /note="EXPERIMENTAL [1]"
FT   misc_RNA        2306..4174
FT                   /note="A.niger glucoamylase gene (encoding GII form
FT                   only) GLAA2 gene; EXPERIMENTAL [2];
FT                   #XOO712 with several modifications; see other
FT                   features; this region contains 4 introns"
FT   sig_peptide     2306..2359
FT                   /note="gpdA secretion signal sequence very similar to
FT                   that of the A. niger glucoamylase gene #X00712;
FT                   EXPERIMENTAL [1] [2]"
FT   CDS             2302..2391
FT                   /note="gpdA secretion signal sequence very similar to
FT                   that of the A. niger glucoamylase gene;
FT                   EXPERIMENTAL [2]"
FT   misc_feature    2360..2378
FT                   /note="PRO_PEPTIDE sequence for putative propeptide,
FT                   contains dibasic processing site (LYS-ARG) [2]"
FT   unsure          2380..4072
FT                   /note="this region was taken from accession X00712.
FT                   GLAA gene present in PAN56-1 is from another A.niger
FT                   (N402) and could therefore have minor deviations from
FT                   the sequence given here [2]"
FT   terminator      4206..4929
FT                   /note="TER A. nidulans trpC gene terminator region
FT                   (containing CDS); EXPERIMENTAL [2],[3];
FT                   contains the 3'end of the trpC
FT                   transcript including 50 codons; two major
FT                   transcription ends have been identified"
FT   misc_difference 4466..4476
FT                   /note="replace(4466..4476,gccttncaggc);
FT                   introduction of one base at this position generates
FT                   an BlgI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 4675..4683
FT                   /note="replace(4675..4683,cagcncctg);
FT                   introduction of one base at this position generates
FT                   an AlwNI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 4763..4773
FT                   /note="replace(4763..4773,gaaatcanttc);
FT                   introduction (actual sequence could be deletion as
FT                   well) of a base at this position results in the
FT                   elimination of an XmnI site which was shown to be
FT                   absent by restriction digestion [3]"
FT   mutation        4800..4807
FT                   /note="replace(4800..4807,catgcatg);
FT                   A NcoI site present at this position was removed by
FT                   digestion, treatment with klenow polymerase and
FT                   religation [2]"
FT   polyA_site      4456..4466
FT                   /note="partial"
FT   polyA_site      4729..4739
FT                   /note="partial"
FT   misc_feature    4929..7564
FT                   /note="from pUC18 HindIII-EcoRI [2]"
FT   misc_feature    1..7564
FT                   /note="fungal expression vector pAN56-1;
FT                   EXPERIMENTAL [2]"
XX
SQ   Sequence 7564 BP; 1800 A; 1987 C; 1868 G; 1906 T; 3 other;
     gaattaattc ccttgtatct ctacacacag gctcaaatca ataagaagaa cggttcgtct
     ttttcgttta tatcttgcat cgtcccaaag ctattggcgg gatattctgt ttgcagttgg
     ctgacttgaa gtaatctctg cagatctttc gacactgaaa tacgtcgagc ctgctccgct
     tggaagcggc gaggagcctc gtcctgtcac aactaccaac atggagtacg ataagggcca
     gttccgccag ctcattaaga gccagttcat gggcgttggc atgatggccg tcatgcatct
     gtacttcaag tacaccaacg ctcttctgat ccagtcgatc atccgctgaa ggcgctttcg
     aatctggtta agatccacgt cttcgggaag ccagcgactg gtgacctcca gcgtcccttt
     aaggctgcca acagctttct cagccagggc cagcccaaga ccgacaaggc ctccctccag
     aacgccgaga agaactggag gggtggtgtc aaggaggagt aagctcctta ttgaagtcgg
     aggacggagc ggtgtcaaga ggatattctt cgactctgta ttatagataa gatgatgagg
     aattggaggt agcatagctt catttggatt tgctttccag gctgagactc tagcttggag
     catagagggt cctttggctt tcaatattct caagtatctc gagtttgaac ttattccctg
     tgaacctttt attcaccaat gagcattgga atgaacatga atctgaggac tgcaatcgcc
     atgaggtttt cgaaatacat ccggatgtcg aaggcttggg gcacctgcgt tggttgaatt
     tagaacgtgg cactattgat catccgatag ctctgcaaag ggcgttgcac aatgcaagtc
     aaacgttgct agcagttcca ggtggaatgt tatgatgagc attgtattaa atcaggagat
     atagcatgat ctctagttag ctcaccacaa aagtcagacg gcgtaaccaa aagtcacaca
     acacaagctg taaggatttc ggcacggcta cggaagacgg agaagccacc ttcagtggac
     tcgagtacca tttaattcta tttgtgtttg atcgagacct aatacagccc ctacaacgac
     catcaaagtc gtatagctac cagtgaggaa gtggactcaa atcgacttca gcaacatctc
     ctggataaac tttaagccta aactatacag aataagatag gtggagagct tataccgagc
     tcccaaatct gtccagatca tggttgaccg gtgcctggat cttcctatag aatcatcctt
     attcgttgac ctagctgatt ctggagtgac ccagagggtc atgacttgag cctaaaatcc
     gccgcctcca ccatttgtag aaaaatgtga cgaactcgtg agctctgtac agtgaccggt
     gactctttct ggcatgcgga gagacggacg gacgcagaga gaagggctga gtaataagcc
     actggccaga cagctctggc ggctctgagg tgcagtggat gattattaat ccgggaccgg
     ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa gaatctattg
     catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg agaatgtgaa
     gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac agaagtccaa
     ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta ggtagagcga
     gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg tccaaatatc
     gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga gctggccagc
     ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca ctcgacctgc
     tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg tgtgtcggcg
     gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct gctctcccca
     ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat cttcccatcc
     aagaaccttt atttccccta agtaagtact ttgctacatc catactccat ccttcccatc
     ccttattcct ttgaaccttt cagttcgagc tttcccactt catcgcagct tgactaacag
     ctaccccgct tgagcagaca tcaccatggg cttccgatct cttctcgccc tgagcggcct
     tgtctgcaac gggttggcaa atgtgatttc caagcgcgcg accttggatt catggttgag
     caacgaagcg accgtggctc gtactgccat cctgaataac atcggggcgg acggtgcttg
     ggtgtcgggc gcggactctg gcattgtcgt tgctagtccc agcacggata acccggactg
     tatgtttcga gctcagattt agtatgagtg tgtcattgat tgattgatgc tgactggcgt
     gtcgtttgtt gtagacttct acacctggac tcgcgactct ggtctcgtcc tcaagaccct
     cgtcgatctc ttccgaaatg gagataccag tctcctctcc accattgaga actacatctc
     cgcccaggca attgtccagg gtatcagtaa cccctctggt gatctgtcca gcggcgctgg
     tctcggtgaa cccaagttca atgtcgatga gactgcctac actggttctt ggggacggcc
     gcagcgagat ggtccggctc tgagagcaac tgctatgatc ggcttcgggc agtggctgct
     tgtatgttct ccaccccctt gcgtctgatc tgtgacatat gtagctgact ggtcaggaca
     atggctacac cagcaccgca acggacattg tttggcccct cgttaggaac gacctgtcgt
     atgtggctca atactggaac cagacaggat atggtgtgtt tgttttattt taaatttcca
     aagatgcgcc agcagagcta acccgcgatc gcagatctct gggaagaagt caatggctcg
     tctttcttta cgattgctgt gcaacaccgc gcccttgtcg aaggtagtgc cttcgcgacg
     gccgtcggct cgtcctgctc ctggtgtgat tctcaggcac ccgaaattct ctgctacctg
     cagtccttct ggaccggcag cttcattctg gccaacttcg atagcagccg ttccggcaag
     gacgcaaaca ccctcctggg aagcatccac acctttgatc ctgaggccgc atgcgacgac
     tccaccttcc agccctgctc cccgcgcgcg ctcgccaacc acaaggaggt tgtagactct
     ttccgctcaa tctataccct caacgatggt ctcagtgaca gcgaggctgt tgcggtgggt
     cggtaccctg aggacacgta ctacaacggc aacccgtggt tcctgtgcac cttggctgcc
     gcagagcagt tgtacgatgc tctataccag tgggacaagc aggggtcgtt ggaggtcaca
     gatgtgtcgc tggacttctt caaggcactg tacagcgatg ctgctactgg cacctactct
     tcgtccagtt cgacttatag tagcattgta gatgccgtga agactttcgc cgatggcttc
     gtctctattg tggtaagtct acgctagaca agcgctcatg ttgacagagg gtgcgtacta
     acagaagtag gaaactcacg ccgcaagcaa cggctccatg tccgagcaat acgacaagtc
     tgatggcgag cagctttccg ctcgcgacct gacctggtct tatgctgctc tgctgaccgc
     caacaaccgt cgtaactccg tcgtgcctgc ttcttggggc gagacctctg ccagcagcgt
     gcccggcacc tgtgcggcca catctgccat tggtacctac agcagtgtga ctgtcacctc
     gtggccgagt atcgtggcta ctggcggcac cactacgacg gctaccccca ctggatccgg
     cagcgtgacc tcgaccagca agaccaccgc gactgctagc aagaccagca ccagtacgtc
     atcaacctcc tgtaccactc ccacggcgcc gtaaagatct gctagaggat ctggttacag
     cttgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct ggatataaga
     tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat ctcgataaga
     tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc tccatgtcaa
     caagaataaa acgcgttttc gggtttacct cttccagata cagctcatct gcaatgcatt
     aatgcattga ctgcaaccta gtaacgcctt ncaggctccg gcgaagagaa gaatagctta
     gcagagctat tttcattttc gggagacgag atcaagcaga tcaacggtcg tcaagagacc
     tacgagactg aggaatccgc tcttggctcc acgcgactat atatttgtct ctaattgtac
     tttgacatgc tcctcttctt tactctgata gcttgactat gaaaattccg tcaccagcnc
     ctgggttcgc aaagataatt gcatgtttct tccttgaact ctcaagccta caggacacac
     attcatcgta ggtataaacc tcgaaatcan ttcctactaa gatggtatac aatagtaacc
     atgcatggtt gcctagtgaa tgctccgtaa cacccaatac gccggccgaa acttttttac
     aactctccta tgagtcgttt acccagaatg cacaggtaca cttgtttaga ggtaatcctt
     ctttctagag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgcct
     gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     ttac
//