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ID PAN71 preliminary; circular DNA; SYN; 6756 BP.
XX
AC Z32698;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAN7-1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 999-2572
RC M13mAN5-7B from M13mp11 & gpdA gene
RC from pAN5-41B
RC from pAN923-41B
RC from pAN923-42B
RA Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT "Functional elements in the promoter region of the Aspergillus
RT nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT dehydrogenase";
RL Gene 93:101-109(1990).
XX
RN [2]
RP 1-6756
RC pAN series from gpdA gene
RA Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA Van Den Hondel C.A.M.J.J.;
RT "Vectors for efficient production of homologous and heterologous
RT proteins in filamentous fungi";
RL Unpublished (1994).
XX
RN [3]
RP 1-6756
RC pAN7-1
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
XX
CC NCBI gi: 475166
CC NM (pAN7-1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN7-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 EcoRI-SalI 2653bp 451..2686..418
FT 2. A.nidulans SalI-EcoRI 4100bp, gpdA gene
FT -> pAN7-1 6756bp"
FT promoter 1..2129
FT /note="PRO A.nidulans gpdA gene;
FT EXPERIMENTAL [1] [2]"
FT misc_feature 2130..2130
FT /note="A.nidulans gpdA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT intron 2178..2293
FT /note=EXPERIMENTAL [1]"
FT CDS 2302..3321
FT /note="hygromycin B phosphotransferase gene (HPH);
FT EXPERIMENTAL [1],[2]"
FT terminator 3339..4108
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 3600..3610
FT /note="replace(3600..3610,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3809..3817
FT /note="replace(3809..3817,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3897..3907
FT /note="replace(3897..3907,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 3934..3941
FT /note="replace(3934..3941,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 3590..3600
FT /note="partial"
FT polyA_site 3863..3873
FT /note="partial"
FT misc_feature 4105..6756
FT /note="from pUC18 SalI-EcoRI [2]"
FT misc_feature 1..6756
FT /note="fungal transformation vector pAN7-1;
FT EXPERIMENTAL [2]"
XX
SQ Sequence 6756 BP; 1642 A; 1737 C; 1693 G; 1681 T; 3 other;
gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
cgtccgaggg caaaggaata gagtagatgc cgaccgcggg atccacttaa cgttactgaa
atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt tttcgggttt
acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa cctagtaacg
ccttncaggc tccggcgaag agaagaatag cttagcagag ctattttcat tttcgggaga
cgagatcaag cagatcaacg gtcgtcaaga gacctacgag actgaggaat ccgctcttgg
ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct tctttactct
gatagcttga ctatgaaaat tccgtcacca gcncctgggt tcgcaaagat aattgcatgt
ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
tcanttccta ctaagatggt atacaatagt aaccatgcat ggttgcctag tgaatgctcc
gtaacaccca atacgccggc cgaaactttt ttacaactct cctatgagtc gtttacccag
aatgcacagg tacacttgtt tagaggtaat ccttctttct agaagtcctc gtgtactgtg
taagcgccca ctccacatct ccactcgacc tgcaggcatg caagcttggc actggccgtc
gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
caatttcaca caggaaacag ctatgaccat gattac
//