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ID   PAN71      preliminary; circular DNA; SYN; 6756 BP.
XX
AC   Z32698;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAN7-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   999-2572
RC   M13mAN5-7B from M13mp11 & gpdA gene
RC   from pAN5-41B
RC   from pAN923-41B
RC   from pAN923-42B
RA   Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA   Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT   "Functional elements in the promoter region of the Aspergillus
RT   nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT   dehydrogenase";
RL   Gene 93:101-109(1990).
XX
RN   [2]
RP   1-6756
RC   pAN series from gpdA gene
RA   Punt P.J., Kuijvenhoven A., Broekhuijsen M.P.,
RA   Van Den Hondel C.A.M.J.J.;
RT   "Vectors for efficient production of homologous and heterologous
RT   proteins in filamentous fungi";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-6756
RC   pAN7-1
RA   Punt P.;
RT   ;
RL   Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL   Rijkswik, The Netherlands.
XX
CC   NCBI gi: 475166
CC   NM (pAN7-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAN7-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 EcoRI-SalI 2653bp 451..2686..418
FT                   2. A.nidulans SalI-EcoRI 4100bp, gpdA gene
FT                   -> pAN7-1 6756bp"
FT   promoter        1..2129
FT                   /note="PRO A.nidulans gpdA gene;
FT                   EXPERIMENTAL [1] [2]"
FT   misc_feature    2130..2130
FT                   /note="A.nidulans gpdA gene major
FT                   transcription start site; EXPERIMENTAL [1]"
FT   intron          2178..2293
FT                   /note=EXPERIMENTAL [1]"
FT   CDS             2302..3321
FT                   /note="hygromycin B phosphotransferase gene (HPH);
FT                   EXPERIMENTAL [1],[2]"
FT   terminator      3339..4108
FT                   /note="TER A. nidulans trpC gene terminator region
FT                   (containing CDS); EXPERIMENTAL [2],[3];
FT                   contains the 3'end of the trpC
FT                   transcript including 50 codons; two major
FT                   transcription ends have been identified"
FT   misc_difference 3600..3610
FT                   /note="replace(3600..3610,gccttncaggc);
FT                   introduction of one base at this position generates
FT                   an BlgI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 3809..3817
FT                   /note="replace(3809..3817,cagcncctg);
FT                   introduction of one base at this position generates
FT                   an AlwNI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 3897..3907
FT                   /note="replace(3897..3907,gaaatcanttc);
FT                   introduction (actual sequence could be deletion as
FT                   well) of a base at this position results in the
FT                   elimination of an XmnI site which was shown to be
FT                   absent by restriction digestion [3]"
FT   mutation        3934..3941
FT                   /note="replace(3934..3941,catgcatg);
FT                   A NcoI site present at this position was removed by
FT                   digestion, treatment with klenow polymerase and
FT                   religation [2]"
FT   polyA_site      3590..3600
FT                   /note="partial"
FT   polyA_site      3863..3873
FT                   /note="partial"
FT   misc_feature    4105..6756
FT                   /note="from pUC18 SalI-EcoRI [2]"
FT   misc_feature    1..6756
FT                   /note="fungal transformation vector pAN7-1;
FT                   EXPERIMENTAL [2]"
XX
SQ   Sequence 6756 BP; 1642 A; 1737 C; 1693 G; 1681 T; 3 other;
     gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
     cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
     cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
     agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
     cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
     ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
     tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
     ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
     ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
     cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
     ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
     gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
     ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
     ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
     acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
     gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
     catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     cgtccgaggg caaaggaata gagtagatgc cgaccgcggg atccacttaa cgttactgaa
     atcatcaaac agcttgacga atctggatat aagatcgttg gtgtcgatgt cagctccgga
     gttgagacaa atggtgttca ggatctcgat aagatacgtt catttgtcca agcagcaaag
     agtgccttct agtgatttaa tagctccatg tcaacaagaa taaaacgcgt tttcgggttt
     acctcttcca gatacagctc atctgcaatg cattaatgca ttgactgcaa cctagtaacg
     ccttncaggc tccggcgaag agaagaatag cttagcagag ctattttcat tttcgggaga
     cgagatcaag cagatcaacg gtcgtcaaga gacctacgag actgaggaat ccgctcttgg
     ctccacgcga ctatatattt gtctctaatt gtactttgac atgctcctct tctttactct
     gatagcttga ctatgaaaat tccgtcacca gcncctgggt tcgcaaagat aattgcatgt
     ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
     tcanttccta ctaagatggt atacaatagt aaccatgcat ggttgcctag tgaatgctcc
     gtaacaccca atacgccggc cgaaactttt ttacaactct cctatgagtc gtttacccag
     aatgcacagg tacacttgtt tagaggtaat ccttctttct agaagtcctc gtgtactgtg
     taagcgccca ctccacatct ccactcgacc tgcaggcatg caagcttggc actggccgtc
     gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     caatttcaca caggaaacag ctatgaccat gattac
//