Return to this vector's summary.
ID PAN81 preliminary; circular DNA; SYN; 6089 BP.
XX
AC Z32751;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pAN8-1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 999-2572
RC M13mAN5-7B from M13mp11 & gpdA gene
RC from pAN5-41B
RC from pAN923-41B
RC from pAN923-42B
RA Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT "Functional elements in the promoter region of the Aspergillus
RT nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT dehydrogenase";
RL Gene 93:101-109(1990).
XX
RN [2]
RP 1-6089
RC pAN series from gpdA gene
RA Punt P.J., Mattern I.E., Van Den Hondel C.A.M.J.J.;
RT "A vector for Aspergillus transformation conferring phleomycin
RT resistance";
RL Fungal Genetics Newsletter 35:25-30(1988).
XX
RN [3]
RP 1-6089
RC pAN8-1
RA Punt P.;
RT ;
RL Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL Rijkswik, The Netherlands.
XX
CC NCBI gi: 475899
CC NM (pAN8-1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pAN8-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 EcoRI-HindIII 2635bp 451..2686..400
FT 2. A.nidulans HindIII-EcoRI 3454bp, gpdA gene
FT -> pAN8-1 6089bp"
FT promoter 1..2129
FT /note="PRO A.nidulans gpdA gene;
FT EXPERIMENTAL [1] [2]"
FT misc_feature 2130..2130
FT /note="A.nidulans gpdA gene major
FT transcription start site; EXPERIMENTAL [1]"
FT intron 2178..2293
FT /note=EXPERIMENTAL [1]"
FT CDS 2302..2676
FT /note="phleomycin resistance gene (ble);
FT EXPERIMENTAL [1],[2]"
FT terminator 2733..3456
FT /note="TER A. nidulans trpC gene terminator region
FT (containing CDS); EXPERIMENTAL [2],[3];
FT contains the 3'end of the trpC
FT transcript including 50 codons; two major
FT transcription ends have been identified"
FT misc_difference 2993..3003
FT /note="replace(2993..3003,gccttncaggc);
FT introduction of one base at this position generates
FT an BlgI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3202..3210
FT /note="replace(3202..3210,cagcncctg);
FT introduction of one base at this position generates
FT an AlwNI site which was shown to be present by
FT restriction digestion [3]"
FT misc_difference 3290..3300
FT /note="replace(3290..3300,gaaatcanttc);
FT introduction (actual sequence could be deletion as
FT well) of a base at this position results in the
FT elimination of an XmnI site which was shown to be
FT absent by restriction digestion [3]"
FT mutation 3327..3334
FT /note="replace(3327..3334,catgcatg);
FT A NcoI site present at this position was removed by
FT digestion, treatment with klenow polymerase and
FT religation [2]"
FT polyA_site 2983..2993
FT /note="partial"
FT polyA_site 3256..3266
FT /note="partial"
FT misc_feature 3456..6089
FT /note="from pUC18 HindIII-EcoRI [2]"
FT misc_feature 1..6089
FT /note="fungal transformation vector pAN8-1;
FT EXPERIMENTAL [2]"
XX
SQ Sequence 6089 BP; 1481 A; 1570 C; 1523 G; 1512 T; 3 other;
gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
cccgcttgag cagacatcac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
ccgacggcgg cccacgggtc ccaggagctt gagatccact taacgttact gaaatcatca
aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt
ccagatacag ctcatctgca atgcattaat gcattgactg caacctagta acgccttnca
ggctccggcg aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc
aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg
cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct
tgactatgaa aattccgtca ccagcncctg ggttcgcaaa gataattgca tgtttcttcc
ttgaactctc aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcanttc
ctactaagat ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac
ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
aggtacactt gtttagaggt aatccttctt tctagagctt ggcactggcc gtcgttttac
aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
acacaggaaa cagctatgac catgattac
//