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ID   PAN81      preliminary; circular DNA; SYN; 6089 BP.
XX
AC   Z32751;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pAN8-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   999-2572
RC   M13mAN5-7B from M13mp11 & gpdA gene
RC   from pAN5-41B
RC   from pAN923-41B
RC   from pAN923-42B
RA   Punt P.J., Dingemanse M., Kuyvenhoven A., Soede R.D.M.,
RA   Pouwels P.H., Van Den Hondel C.A.M.J.J.;
RT   "Functional elements in the promoter region of the Aspergillus
RT   nidulans GPDA gene encoding glyceraldehyde-3-phosphate
RT   dehydrogenase";
RL   Gene 93:101-109(1990).
XX
RN   [2]
RP   1-6089
RC   pAN series from gpdA gene
RA   Punt P.J., Mattern I.E., Van Den Hondel C.A.M.J.J.;
RT   "A vector for Aspergillus transformation conferring phleomycin
RT   resistance";
RL   Fungal Genetics Newsletter 35:25-30(1988).
XX
RN   [3]
RP   1-6089
RC   pAN8-1
RA   Punt P.;
RT   ;
RL   Submitted (22-APR-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Punt P., TNO Nutrition and Food Research Institute, Lange Kleiweg 139,
RL   Rijkswik, The Netherlands.
XX
CC   NCBI gi: 475899
CC   NM (pAN8-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pAN8-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 EcoRI-HindIII 2635bp 451..2686..400
FT                   2. A.nidulans HindIII-EcoRI 3454bp, gpdA gene
FT                   -> pAN8-1 6089bp"
FT   promoter        1..2129
FT                   /note="PRO A.nidulans gpdA gene;
FT                   EXPERIMENTAL [1] [2]"
FT   misc_feature    2130..2130
FT                   /note="A.nidulans gpdA gene major
FT                   transcription start site; EXPERIMENTAL [1]"
FT   intron          2178..2293
FT                   /note=EXPERIMENTAL [1]"
FT   CDS             2302..2676
FT                   /note="phleomycin resistance gene (ble);
FT                   EXPERIMENTAL [1],[2]"
FT   terminator      2733..3456
FT                   /note="TER A. nidulans trpC gene terminator region
FT                   (containing CDS); EXPERIMENTAL [2],[3];
FT                   contains the 3'end of the trpC
FT                   transcript including 50 codons; two major
FT                   transcription ends have been identified"
FT   misc_difference 2993..3003
FT                   /note="replace(2993..3003,gccttncaggc);
FT                   introduction of one base at this position generates
FT                   an BlgI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 3202..3210
FT                   /note="replace(3202..3210,cagcncctg);
FT                   introduction of one base at this position generates
FT                   an AlwNI site which was shown to be present by
FT                   restriction digestion [3]"
FT   misc_difference 3290..3300
FT                   /note="replace(3290..3300,gaaatcanttc);
FT                   introduction (actual sequence could be deletion as
FT                   well) of a base at this position results in the
FT                   elimination of an XmnI site which was shown to be
FT                   absent by restriction digestion [3]"
FT   mutation        3327..3334
FT                   /note="replace(3327..3334,catgcatg);
FT                   A NcoI site present at this position was removed by
FT                   digestion, treatment with klenow polymerase and
FT                   religation [2]"
FT   polyA_site      2983..2993
FT                   /note="partial"
FT   polyA_site      3256..3266
FT                   /note="partial"
FT   misc_feature    3456..6089
FT                   /note="from pUC18 HindIII-EcoRI [2]"
FT   misc_feature    1..6089
FT                   /note="fungal transformation vector pAN8-1;
FT                   EXPERIMENTAL [2]"
XX
SQ   Sequence 6089 BP; 1481 A; 1570 C; 1523 G; 1512 T; 3 other;
     gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
     cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
     cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
     agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
     cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
     ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
     tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
     ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
     ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
     cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
     ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
     gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
     ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
     ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
     acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
     gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
     catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     cccgcttgag cagacatcac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
     ccgacggcgg cccacgggtc ccaggagctt gagatccact taacgttact gaaatcatca
     aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
     caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
     tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgttttcggg tttacctctt
     ccagatacag ctcatctgca atgcattaat gcattgactg caacctagta acgccttnca
     ggctccggcg aagagaagaa tagcttagca gagctatttt cattttcggg agacgagatc
     aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg
     cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct
     tgactatgaa aattccgtca ccagcncctg ggttcgcaaa gataattgca tgtttcttcc
     ttgaactctc aagcctacag gacacacatt catcgtaggt ataaacctcg aaatcanttc
     ctactaagat ggtatacaat agtaaccatg catggttgcc tagtgaatgc tccgtaacac
     ccaatacgcc ggccgaaact tttttacaac tctcctatga gtcgtttacc cagaatgcac
     aggtacactt gtttagaggt aatccttctt tctagagctt ggcactggcc gtcgttttac
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     acacaggaaa cagctatgac catgattac
//