ID   PASK22     preliminary; circular DNA; SYN; 3523 BP.
XX
AC   A13392; A13393;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pASK22 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3523
RC   pASK22 from pBR322
RA   Plueckthun A., Skerra A.;
RT   "Method of producing antibodies by genetic engineering";
RL   Unpublished (1986).
RL   European Patent No. 0324162-A 1 dated 19-JUL-1989.
RL   Plueckthun, Andreas, Dr.; Skerra, Arne.
XX
CC   NCBI gi: 491655
CC   NM (pASK22)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pASK22 3523bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3523 BP; 858 A; 901 C; 877 G; 887 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aatttctaga
     taacgagggc aaaaaatgaa aaagacagct atcgcgattg cagtggcact ggctggtttc
     gctaccgtag cgcaggccga agttaaactg gtagagtctg gtggtggtct ggtacagccg
     ggtggatccc tgcgtctgtc ttgcgctacc tcaggtttca ccttctctga cttctacatg
     gagtgggtac gtcagccccc gggtaaacgt ctcgagtgga tcgcagctag ccgtaacaaa
     ggtaacaagt ataccaccga atacagcgct tctgttaaag gtcgtttcat cgtttctcgt
     gacactagtc aatcgatcct gtacctgcag atgaatgcat tgcgtgctga agacaccgct
     atctactact gcgcgcgtaa ctactatggc agcacttggt acttcgacgt ttggggtgca
     ggtaccaccg ttaccgtttc ttcttgataa catggagaaa ataaagtgaa acaaagcact
     attgcactgg cactcttacc gttactgttt acccctgtga caaaagccga tatcgttatg
     acccagtctc cgagctctct gtctgtatct gcaggtgaac gtgttaccat gtcttgcaaa
     tcttctcagt ctctgctgaa ctctggtaac cagaaaaact tcctggcgtg gtatcagcaa
     aagcctggcc aaccgccgaa actgctgatc tacggtgcgt cgacccgtga atctggtgtt
     ccggaccgtt ttaccggtag cggtagcggt accgacttca ctctgaccat ctcttctgta
     caggctgaag atctggctgt ttactactgt caaaacgacc actcttaccc gctgaccttt
     ggcgccggca ccaaactgga actgaagcgc gcttgataag cttggcactg gccgtcgttt
     tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//