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ID   PAST19B    preliminary; circular DNA; SYN; 3052 BP.
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AC   X53124;
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DT   15-AUG-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   C. elegans phagemid vector pAST19b - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3052
RC   pAST19b
RA   Fire A.Z.;
RT   ;
RL   Submitted (17-MAY-1990) on tape to the EMBL Data Library by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
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RN   [2]
RP   1-3052
RC   pAST18a from pAST & pUC18
RC   pAST18b from pAST & pUC18
RC   pAST19a from pAST & pUC19
RC   pAST19b from pAST & pUC19
RC   pICT19h from pAST & pIC19H
RC   pICT19r from pAST & pIC19R
RC   pPD26.14 from pAST19b & linker
RA   Fire A.Z., Kondo K., Waterson R.;
RT   "Vectors for low copy transformation of C. elegans";
RL   Nucleic Acids Res. 18:4269-4270(1990).
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CC   pAST19b is made from C. elegans sup-7 sequence (K02459) and
CC   pUC19 sequence (M11662).
CC   NM (pAST19b)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (C.elegans)
CC   CP (low)
CC   FN (cloning)
CC   SE ()
CC   PA (pUC19)(C.elegans sup-7)
CC   BR (pAST18a)(pAST18b)(pAST19a)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. C.elegans, sup-7 locus
FT                   -> pRW91
FT                   1. pRW91 EcoRI-SalI 1300bp, C.elegans sup-7
FT                   2. pUC12 remove EcoRI-SalI 30bp 231..261, MCS/2650bp
FT                   -> pAst 3950bp
FT                   1. pUC19 SspI 2686bp 2504..2504
FT                   2. pAst SspI-SspI 366bp, sup-7
FT                   -> pAST19B 3052bp"
FT   misc_feature    1..2501
FT                   /note="from pUC19"
FT   misc_feature    2502..2869
FT                   /note="C. elegans sup-7 locus"
FT   misc_feature    2870..3052
FT                   /note="from pUC19"
XX
SQ   Sequence 3052 BP; 797 A; 733 C; 747 G; 775 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
     cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatgacaaga gagaataaca
     tgactgcttg gcgcaatggt agcgcgttcg actctagatc gaaaggttgg gcgttcgatc
     cgctcagtgg tcaaattttt tgttttttaa gtagtaatat aatacaattt aattcaaaat
     tacacgcaaa aatttaataa agtagtccaa aatgctaatc gtgtgaaaaa atgccttctg
     gtaatgtatt tctcacacga aaatttgttg aaattggttt ttgacacaag cgaatgttgc
     aattattttc ttgatttttc tacaaatttg aaaatcaaat ttcacgactg caagtataac
     attaaaaaca atatacccaa gcccacaaaa aacgtattct gaaaattgta ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//