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ID   PBACPAK1   preliminary; circular DNA; SYN; 5461 BP.
XX
AC   IG1033;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pBacPAK1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBacPAK1, pBacPAK8, pBacPAK9 from AcNPV
RA   Kitts P.A., Possee R.D.;
RT   "A method for producing recombinant baculovirus expression vectors
RT   at high frequency";
RL   Biotechniques 14:810-817(1993).
XX
CC   NM (pBacPAK1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (Spodoptera frugiperda IPLB-SF21)
CC   CP ()
CC   FN (transfer)(expression)
CC   SE ()
CC   PA (pAcYM1)(pAcCL29)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. AcNPV, polyhedrin gene
FT                   2. pBR322, ori/amp gene
FT                   3. M13, ori
FT                   -> pBacPAK1 5461bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    314..314
FT                   /note="SIT BstXI"
FT   misc_binding    546..546
FT                   /note="SIT MluI"
FT   misc_binding    1158..1158
FT                   /note="SIT EcoRV"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    1253..1253
FT                   /note="SIT BamHI"
FT   misc_binding    1324..1324
FT                   /note="SIT SnaBI"
FT   polyA_signal    0..0
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   misc_binding    1687..1687
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    2998..2998
FT                   /note="SIT DraIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3913..3913
FT                   /note="SIT ScaI"
FT   misc_binding    4873..4873
FT                   /note="SIT AlwNI"
XX
SQ   Sequence 5461 BP; 1432 A; 1184 C; 1258 G; 1587 T; 0 other;
     aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
     aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
     caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
     accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
     ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
     cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
     accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
     caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
     gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
     gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
     ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
     tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
     ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
     ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
     tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
     ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
     acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccggg
     ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     aaataaagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
     gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
     acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
     aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa
     ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc
     tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg
     ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc
     aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt
     ttttggtttg accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa
     ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg
     cggcaattca gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg
     agaaggtggt ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac
     gattgtgggc accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg
     cagcgcttgg ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc
     tataagcatt gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta
     agcaattgta ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa
     gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgcctgatg
     cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     g
//