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ID   PBACPAK8   preliminary; circular DNA; SYN; 5538 BP.
XX
AC   IG1433;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pBacPAK8 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBacPAK1, pBacPAK8, pBacPAK9 from AcNPV
RA   Kitts P.A., Possee R.D.;
RT   "A method for producing recombinant baculovirus expression vectors
RT   at high frequency";
RL   Biotechniques 14:810-817(1993).
XX
CC   NM (pBacPAK8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (Spodoptera frugiperda IPLB-SF21)
CC   CP ()
CC   FN (transfer)(expression)
CC   SE ()
CC   PA (pAcYM1)(pAcCL29)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. AcNPV, polyhedrin gene
FT                   2. pBR322, ori/amp gene
FT                   3. M13, ori
FT                   -> pBacPAK8 5538bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    314..314
FT                   /note="SIT BstXI"
FT   misc_binding    546..546
FT                   /note="SIT MluI"
FT   misc_binding    1158..1158
FT                   /note="SIT EcoRV"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    1253..1253
FT                   /note="SIT BamHI"
FT   misc_binding    1324..1324
FT                   /note="SIT SnaBI"
FT   polyA_signal    0..0
FT                   /note="PLA AcMNPV polyhedrin gene"
FT   misc_binding    1687..1687
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   misc_binding    2998..2998
FT                   /note="SIT DraIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3913..3913
FT                   /note="SIT ScaI"
FT   misc_binding    4873..4873
FT                   /note="SIT AlwNI"
XX
SQ   Sequence 5538 BP; 1449 A; 1205 C; 1278 G; 1606 T; 0 other;
     aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
     aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
     caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
     accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
     ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
     cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
     accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
     caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
     gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
     gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
     ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
     tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
     ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
     ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
     tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
     ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
     acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg
     caggcctcga gttcgaatct agaagatctg gtaccgagct cgaattcccg ggcggccgct
     taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
     tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
     tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
     tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
     ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
     agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
     tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
     ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
     ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
     tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
     gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
     actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
     atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
     ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
     gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
     ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
     ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
     ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
     ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
     ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
     ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
     aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
     aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
     ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
     gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     gccgattcat taatgcag
//