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ID   PBBR1MCS   preliminary; circular DNA; SYN; 4707 BP.
XX
AC   U02374;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pBBR1MCS - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4707
RC   pBBR1 from Bordetella bronchiseptica
RC   pBBR1MCS from pBluescript II KS- & pBBR1 & pBR328
RA   Kovach M.E., Phillips R.W., Elzer P.H., Roop R.M., Peterson K.M.;
RT   "pBBR1MCS:  A broad-host-range cloning vector";
RL   Biotechniques 16:800-802(1994).
XX
RN   [2]
RP   1-4707
RC   pBBR1MCS
RA   Kovach M.E.;
RT   ;
RL   Submitted (05-OCT-1993) by:
RL   Kovach M.E., Microbiology & Immunology, Louisiana State University,
RL   Medical Center-Shreveport, 1501 Kings Hwy., Shreveport, LA 71130, USA.
XX
CC   NCBI gi: 433345
CC   NM (pBBR1MCS)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Bordetella bronchiseptica)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA (pBluescript II KS-)(pBBR1)(pBR328)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Bordetella bronchiseptica
FT                   -> pBBR1
FT                   1. pBBR1 2516bp 1..2517
FT                   2. pBR328 RsaI-NarI 427bp 4108..4535
FT                   3. pBluescript II KS- ?-HaeII 650bp 381..1031
FT                   4. oligo 10bp ttttatgcat
FT                   5. pBR328 ?-RsaI 939bp 3168..4107
FT                   6. pBBR1 167bp 2520..2687
FT                   -> pBBR1MCS 4707bp"
FT   misc_feature    1..2517
FT                   /note="bp 1 to 2517 of pBBR1 GenBank Accession Number
FT                   X66730"
FT   misc_feature    2516..2943
FT                   /note="complement of bp 4108 to 4535 of pBR328 GenBank
FT                   Accession Number L08858"
FT   misc_feature    2943..3593
FT                   /note="bp 381 to 1031 of pBluescript II KS- GenBank
FT                   Accession Number X52329"
FT   misc_feature    3604..3613
FT                   /note="complement of 5' end of PCR primer (5'
FT                   TTTTATGCATgcgcccaatacgcaaa 3') used to amplify
FT                   pBluescript II KS- polylinker"
FT   misc_feature    3604..4543
FT                   /note="complement of bp 3168 to 4107 of pBR328 Genbank
FT                   Accession Number L08858"
FT   misc_feature    4540..4707
FT                   /note="bp 2520 to 2687 of pBBR1 GenBank Accession
FT                   Number X66730"
FT   rep_origin      0..0
FT                   /note="ORI Bordetella bronchiseptica"
XX
SQ   Sequence 4707 BP; 966 A; 1380 C; 1329 G; 1032 T; 0 other;
     ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
     cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
     ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
     cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
     cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
     gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
     tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
     tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
     acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
     ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
     tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
     ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
     ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
     tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
     cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
     cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
     ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
     aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt
     cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc
     gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg
     ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca
     gaacgggctt caggcgctcc cgaaggt
//