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ID   PBCSKM     preliminary; circular DNA; SYN; 2293 BP.
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AC   IG2063; X52330; S52394; X52324;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBC SK(-) - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2961
RC   pBC SK(-)
RA   Thomas E.A.;
RT   ;
RL   Submitted (20-FEB-1990) on tape to the EMBL Data Library by:
RL   Thomas E.A., Stratagene Cloning Systems,
RL   11099 North Torrey Pines Rd., La Jolla, CA 92037, USA.
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RN   [2]
RC   pBluescript SK+ from pUC19
RC   pBluescript SK- from pUC19
RC   pBluescript KS+ from pUC19
RC   pBluescript KS- from pUC19
RC   pBluescript II SK+ from pUC19
RC   pBluescript II SK- from pUC19
RC   pBluescript II KS+ from pUC19
RC   pBluescript II KS- from pUC19
RC   lambda ZAP I from pBluescript SK- & lambda
RC   lambda ZAP II from pBluescript SK- & lambda
RC   pBC KS+ from pBluescript KS+ & cat gene
RC   pBC KS- from pBluescript KS- & cat gene
RC   pBC SK+ from pBluescript SK+ & cat gene
RC   pBC SK- from pBluescript SK- & cat gene
RA   Alting-Mees M.A., Sorge J.A., Short J.M.;
RT   "pBluescriptII: multifunctional cloning and mapping vectors";
RL   Meth. Enzymol. 216:483-495(1992).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   The SK designation indicates the polylinker is oriented such
CC   that beta-galactosidase (lacZ) transcription proceeds through the SacI
CC   site first and the KpnI site last.
CC   pBluescript SK(+) carries an F1 origin of replication,
CC   oriented such that transcription proceeds in
CC   the same direction as beta-galactosidase (lacZ) transcription.
CC   NM (pBC SK-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)
CC   CP ()
CC   FN (mapping gene)(expression)
CC   SE (color blue/white)
CC   PA (pBluescript II SK-)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript SK- remove TaqI-TaqI 1444bp
FT                   \ 1254..2698, amp gene/1520bp, amp gene
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pBC SK- 2293bp"
FT   -               1..1253
FT                   /note="pBluescript SK- 1..1253 1253bp
FT                   TaqI = T^CGA"
FT   -               1254..2026
FT                   /note="Tn9 215..987 773bp
FT                   TaqI = T^CGA"
FT   -               2027..2293
FT                   /note="pBluescript SK- 2698..2964 267bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS (21 unique restriction sites)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 2293 BP; 571 A; 550 C; 564 G; 608 T; 0 other;
     ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct
     gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
     gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgagatt
     ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat gtaacccact
     cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctg
//