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ID   PBD12      preliminary; circular DNA; SYN; 6667 BP.
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AC   ATCC37101;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pBD12 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBD6 from pSA0501 & pUB110
RC   pBD7 from B.licheniformis DNA & pUB110
RC   pBD8 from pUB110 & pSA2100
RC   pBD9 from pUB110 & pE194
RC   pBD10, pBD11 from pBD8 & pE194
RC   pBD12 from pUB110 & pCM194
RA   Gryczan T.J., Dubnau D.;
RT   "Construction and properties of chimeric plasmids in Bacillus
RT   subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1428-1432(1978).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   A general purpose plasmid vector for cloning in Bacillus subtilis. [2]
CC   Medium is 1179 V Y medium.
CC   NM (pBD12)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pC194)
CC   BR (pBD6)(pBD8)(pBD9)(pBD10)(pBD11)(pBD20)(pBD35)
CC   OF (pBD64)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pC194 2910bp
FT                   -> pCM194 2910bp
FT                   1. pUB110 HpaII-HpaII 3757bp 2753..4548..1962,
FT                   \ kan gene/HpaII B fragment
FT                   2. pCM194 HpaII 2910bp 974..974, cat gene
FT                   -> pBD12 6667bp"
FT   -               1..973
FT                   /note="pC194 1..973 973bp
FT                   HpaII = C^CGG"
FT   -               974..4730
FT                   /note="pUB110 2753..4548..1961 3757bp
FT                   HpaII = C^CGG"
FT   -               4731..6667
FT                   /note="pC194 974..2910 1937bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-XbaI-BamHI-TacI-HindIII-
FT                   BglII"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   CDS             0..0
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
XX
SQ   Sequence 6667 BP; 2037 A; 1301 C; 860 G; 2469 T; 0 other;
     gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
     ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
     ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
     aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
     cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
     gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
     tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
     tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
     tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
     ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
     cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
     gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
     tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
     tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
     aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
     ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
     taactagcct cgccggttgt ccattcatgg ctgaactctg cttcctctgt tgacatgaca
     cacatcatct caatatccga atagggccca tcagtctgac gaccaagaga gccataaaca
     ccaatagcct taacatcatc cccatattta tccaatattc gttccttaat ttcatgaaca
     atcttcattc tttcttctct agtcattatt attggtccat tcactattct cattcccttt
     tcagataatt ttagatttgc ttttctaaat aagaatattt ggagagcacc gttcttattc
     agctattaat aactcgtctt cctaagcatc cttcaatcct tttaataaca attatagcat
     ctaatcttca acaaactggc ccgtttgttg aactactctt taataaaata atttttccgt
     tcccaattcc acattgcaat aatagaaaat ccatcttcat cggctttttc gtcatcatct
     gtatgaatca aatcgccttc ttctgtgtca tcaaggttta attttttatg tatttctttt
     aacaaaccac cataggagat taacctttta cggtgtaaac cttcctccaa atcagacaaa
     cgtttcaaat tcttttcttc atcatcggtc ataaaatccg tatcctttac aggatatttt
     gcagtttcgt caattgccga ttgtatatcc gatttatatt tatttttcgg tcgaatcatt
     tgaactttta catttggatc atagtctaat ttcattgcct ttttccaaaa ttgaatccat
     tgtttttgat tcacgtagtt ttctgtattc ttaaaataag ttggttccac acataccaat
     acatgcatgt gctgattata agaattatct ttattattta ttgtcacttc cgttgcacgc
     ataaaaccaa caagattttt attaattttt ttatattgca tcattcggcg aaatccttga
     gccatatctg acaaactctt atttaattct tcgccatcat aaacattttt aactgttaat
     gtgagaaaca accaacgaac tgttggcttt tgtttaataa cttcagcaac aaccttttgt
     gactgaatgc catgtttcat tgctctcctc cagttgcaca ttggacaaag cctggattta
     caaaaccaca ctcgatacaa ctttctttcg cctgtttcac gattttgttt atactctaat
     atttcagcac aatcttttac tctttcagcc tttttaaatt caagaatatg cagaagttca
     aagtaatcaa cattagcgat tttcttttct ctccatggtc tcacttttcc actttttgtc
     ttgtccacta aaacccttga tttttcatct gaataaatgc tactattagg acacataata
     ttaaaagaaa cccccatcta tttagttatt tgtttagtca cttataactt taacagatgg
     ggtttttctg tgcaaccaat tttaagggtt ttcaatactt taaaacacat acataccaac
     acttcaacgc acctttcagc aactaaaata aaaatgacgt tatttctata tgtatcaaga
     taagaaagaa caagttcaaa accatcaaaa aaagacacct tttcaggtgc tttttttatt
     ttataaactc attccctgat ctcgacttcg ttcttttttt acctctcggt tatgagttag
     ttcaaattcg ttctttttag gttctaaatc gtgtttttct tggaattgtg ctgttttatc
     ctttaccttg tctacaaacc ccttaaaaac gtttttaaag gcttttaagc cgtctgtacg
     ttccttaagg aattccttag tgctttcata gattaaactc acatcacgct ttaaatcgct
     tattttagac tttaaagact tgttttcttc aagcaactca ttataatcat ttacattttc
     attaaatcgc tctacaagac cactatattt ttctttaact tgcccatgtt ctttacttaa
     ttttttatat tctctcgcca tatcagtact catgagattt ctaacatgct gttttaacct
     atcgttatct ctcgcagcag tcactaagtt tttataatca cgctccgata taacaacatt
     tttggttggt ttcttttctg ttttcattat ttcttttccc aaaccaaaca tggacttttc
     acccgttggc acttcaacac ttttcatgtg tcgtttcgct ggtacttcta aatctgattt
     aactttatcg ctataagcag tccattcatc ttttttaact gctaaatttt tttctagaaa
     atcaatctct ttttccaaag tttgtttttt aaatttagct gtctcaatat gtttacggtc
     agagccacgt tcaccacgct tcaactcaaa accctgtttt ttcatatgct cggggaattt
     atcttgtagc cataacagtt cttgacgatt aaacacattt tttccttgca gttttccatc
     acgcataggc acaacaccta aatgcatgtg aggggtttgc tcatcattat gaactgttgc
     ataagcaata ttttgcttgc catatcgttc ggaaaataat ttataacttt cctcaaaaaa
     tcgtttttgt tctcctggat ccagttgctc aaaaaaatct cggtcagatg ttactagcaa
     ctcatttaca agaacagcat ctttcctccg tttttcttgt acctgttttt tgtgattcaa
     taatttcttt gacacgttcg ttgtaatcaa tatttttatc atttttcaaa tcataatttt
     cacgtgttcg ctcatggtca atatcatcat tcgttctact ttttcgctct ctttgattat
     gaaattgcat gccttttagt ccagctgatt tcactttttg cattctacaa actgcataac
     tcatatgtaa atcgctcctt tttaggtggc acaaatgtga ggcattttcg ctctttccgg
     caaccacttc caagtaaagt ataacacact atactttata ttcataaagt gtgtgctctg
     cgaggctgtc ggcagtgccg accaaaacca taaaaccttt aagacctttc ttttttttac
     gagaaaaaag aaacaaaaaa acctgccctc tgccacctca gcaaaggggg gttttgctct
     cgtgctcgtt taaaaatcag caagggacag gtagtatttt ttgagaagat cactcaaaaa
     atctccacct ttaaaccctt gccaattttt attttgtccg ttttgtctag cttaccgaaa
     gccagactca gcaagaataa aatttttatt gtctttcggt tttctagtgt aacggacaaa
     accactcaaa ataaaaaaga tacaagagag gtctctcgta tcttttattc agcaatcgcg
     cccgattgct gaacagatta ataatagatt ttagcttttt atttgttgaa aaaagctaat
     caaattgttg tcgggatcaa ttactgcaaa gtctcgttca tcccaccact gatcttttaa
     tgatgtattg gggtgcaaaa tgcccaaagg cttaatatgt tgatataatt catcaattcc
     ctctacttca atgcggcaac tagcagtacc agcaataaac gactccgcac ctgtacaaac
     cggtgaatca ttactacgag agcgccagct tcatcacttg cctcccatag atgaatccga
     acctcattac acattagaac tgcgaatcca tcttcatggt gaaccaaagt gaaacctagt
     ttatcgcaat aaaaacctat actcttttta atatccccga ctggcaatgc cggcaatagt
     tacccttatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa
     aaacacaaaa gaccacattt tttaatgtgg tctttattct tcaactaaag cacccattag
     ttcaacaaac gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt
     aatattgact tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta
     aattcacttt agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta
     gacaattgga agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt
     ataaccacag aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa
     ttttaccctg catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga
     actggttaca atagcgacgg agagttaggt tattgggata agttagagcc actttataca
     atttttgatg gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc
     aaagagtttt atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg
     tttcccaaaa cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca
     tttactgggt ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca
     gcaggaaaat tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat
     cattctgttt gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca
     gataggccta atgactggct tttataatat gagataatgc cgactgtact ttttacagtc
     ggttttctaa tgtcactaac ctgccccgtt agttgaagaa ggtttttata ttacagctcc
     agatccatat ccttcttttt ctgaaccgac ttctcctttt tcgcttcttt attccaattg
     ctttattgac gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct
     taatagctca cgctatgccg acattcgtct gcaagtttag ttaagggttc ttctcaacgc
     acaataaatt ttctcggcat aaatgcgtgg tctaattttt atttttaata accttgatag
     caaaaaatgc cattccaata caaaaccaca tacctataat cgataaccac ataacagtca
     taaaaccact cctttttaac aaactttatc acaagaaata tttaaatttt aaatgccttt
     attttgaatt ttaaggggca ttttaaagat ttaggggtaa atcatatagt tttatgccta
     aaaacctaca gaagctttta aaaagcaaat atgagccaaa taaatatatt ctaattctac
     aaacaaaaat ttgagcaaat tcagtgtcga ttttttaaga cactgcccag ttacatgcaa
     attaaaattt tcatgatttt ttatagttcc taacagggtt aaaatttgta taacgaaagt
     ataatgttta tataacgtta gtataataaa gcattttaac attatacttt tgataatcgt
     ttatcgtcgt catcacaata acttttaaaa tactcgtgca taattcacgc tgacctccca
     ataactacat ggtgttatcg ggaggtcagc tgttagcact tatattttgt tattgttctt
     cctcgatttc gtctatcatt ttgtgattaa tttctctttt ttcttgttct gttaagtcat
     aaagttcact agctaaatac tctttttgtt tccaaatata aaaaatttga tagatatatt
     acggttg
//