back Return to this vector's summary.
ID   PBD64      preliminary; circular DNA; SYN; 4780 BP.
XX
AC   X51450; ATCC37104;
XX
DT   18-APR-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/Staphylococcus plasmid vector pBD64 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4780
RC   pBD64
RA   Minton N.P.;
RT   ;
RL   Submitted (16-JAN-1990) on tape to the EMBL Data Library by:
RL   Minton N.P., Centre for Applied Microbiology and Research,
RL   Molecular Genetics Group, Division of Biotechnology PHLS CAMR,
RL   Porton Down, Salisbury Wiltshire SP4 0JG, United Kingdom.
XX
RN   [2]
RP   1-4780
RC   pBD12 from pUB110 & pC194
RC   pBD64 from pBD12
RA   Minton N.P., Swinfield T.J., Brehm J.K., Oultram J.D.;
RT   "The Gram positive cloning vector pBD64 arose by a 1844 bp
RT   deletion of pC194 derived DNA";
RL   Nucleic Acids Res. 18:1651-1651(1990).
XX
RN   [3]
RC   pBD40 from pBD9
RC   pBD63 from pBD6 & pHV11
RC   pBD64 from pBD12
RA   Gryczan T.J., Shivakumar A.G., Dubnau D.;
RT   "Characterization of chimeric plasmid cloning vehicles in Bacillus
RT   subtilis";
RL   J. Bacteriol. 141:246-253(1980).
XX
CC   pBD64 is a chimeric vector composed of plasmids pUB110 and pC194,
CC   with a 1844 bp deletion in the pC194 derived DNA.
CC   This vector was isolated as a spontaneous deletion variant of pBD12,
CC   a chimeric vector composed of plasmids pUB110 and pC194. The deletion
CC   consists of 1844 bp (nt 3803 to 5649) of pBD12, & encompasses
CC   nt 1 to 487 & 1552 to 2906 of the pC194 sequence. [2]
CC   Medium is 1179 V Y medium.
CC   NM (pBD64)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (B.subtilis BD170)(B.subtilis)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBD12 from pUB110 and pC194)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBD12 remove 1887bp, pC194/not cat/4780bp
FT                   -> pBD64 4780bp"
FT   CDS             complement(242..1012)
FT                   /note="ANT Bacillus neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan) (AA 1-256)"
FT   CDS             complement(1172..2155)
FT                   /note="GEN Bacillus repB gene (AA 1-327)"
FT   rep_origin      complement(2335..2335)
FT                   /note="ORI Bacillus pUB110"
FT   misc_feature    3803..3804
FT                   /note="site of deleted pC194 DNA"
FT   CDS             complement(3847..4497)
FT                   /note="ANT Bacillus chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)
FT                   (AA 1-216)"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique TacI"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
XX
SQ   Sequence 4780 BP; 1399 A; 976 C; 648 G; 1757 T; 0 other;
     ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta atgtaaatct
     attaccttat tattaattca attcgctcat aattaatcct ttttcttatt acgcaaaatg
     gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg ataattacta
     atactaggag aagttaataa atacgtaacc aacatgatta acaattatta gaggtcatcg
     ttcaaaatgg tatgcgtttt gacacatcca ctatatatcc gtgtcgttct gtccactcct
     gaatcccatt ccagaaattc tctagcgatt ccagaagttt ctcagagtcg gaaagttgac
     cagacattac gaactggcac agatggtcat aacctgaagg aagatctgat tgcttaactg
     cttcagttaa gaccgaagcg ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca
     tggcacctgc cattgctacc tgtacagtca aggatggtag aaatgttgtc ggtccttgca
     cacgaatatt acgccatttg cctgcatatt caaacagctc ttctacgata agggcacaaa
     tcgcatcgtg gaacgtttgg gcttctaccg atttagcagt ttgatacact ttctctaagt
     atccacctga atcataaatc ggcaaaatag agaaaaattg accatgtgta agcggccaat
     ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa ttcacttcca
     ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt gacatgacac
     acatcatctc aatatccgaa tagggcccat cagtctgacg accaagagag ccataaacac
     caatagcctt aacatcatcc ccatatttat ccaatattcg ttccttaatt tcatgaacaa
     tcttcattct ttcttctcta gtcattatta ttggtccatt cactattctc attccctttt
     cagataattt tagatttgct tttctaaata agaatatttg gagagcaccg ttcttattca
     gctattaata actcgtcttc ctaagcatcc ttcaatcctt ttaataacaa ttatagcatc
     taatcttcaa caaactggcc cgtttgttga actactcttt aataaaataa tttttccgtt
     cccaattcca cattgcaata atagaaaatc catcttcatc ggctttttcg tcatcatctg
     tatgaatcaa atcgccttct tctgtgtcat caaggtttaa ttttttatgt atttctttta
     acaaaccacc ataggagatt aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac
     gtttcaaatt cttttcttca tcatcggtca taaaatccgt atcctttaca ggatattttg
     cagtttcgtc aattgccgat tgtatatccg atttatattt atttttcggt cgaatcattt
     gaacttttac atttggatca tagtctaatt tcattgcctt tttccaaaat tgaatccatt
     gtttttgatt cacgtagttt tctgtattct taaaataagt tggttccaca cataccaata
     catgcatgtg ctgattataa gaattatctt tattatttat tgtcacttcc gttgcacgca
     taaaaccaac aagattttta ttaatttttt tatattgcat cattcggcga aatccttgag
     ccatatctga caaactctta tttaattctt cgccatcata aacattttta actgttaatg
     tgagaaacaa ccaacgaact gttggctttt gtttaataac ttcagcaaca accttttgtg
     actgaatgcc atgtttcatt gctctcctcc agttgcacat tggacaaagc ctggatttac
     aaaaccacac tcgatacaac tttctttcgc ctgtttcacg attttgttta tactctaata
     tttcagcaca atcttttact ctttcagcct ttttaaattc aagaatatgc agaagttcaa
     agtaatcaac attagcgatt ttcttttctc tccatggtct cacttttcca ctttttgtct
     tgtccactaa aacccttgat ttttcatctg aataaatgct actattagga cacataatat
     taaaagaaac ccccatctat ttagttattt gtttagtcac ttataacttt aacagatggg
     gtttttctgt gcaaccaatt ttaagggttt tcaatacttt aaaacacata cataccaaca
     cttcaacgca cctttcagca actaaaataa aaatgacgtt atttctatat gtatcaagat
     aagaaagaac aagttcaaaa ccatcaaaaa aagacacctt ttcaggtgct ttttttattt
     tataaactca ttccctgatc tcgacttcgt tcttttttta cctctcggtt atgagttagt
     tcaaattcgt tctttttagg ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc
     tttaccttgt ctacaaaccc cttaaaaacg tttttaaagg cttttaagcc gtctgtacgt
     tccttaagga attccttagt gctttcatag attaaactca catcacgctt taaatcgctt
     attttagact ttaaagactt gttttcttca agcaactcat tataatcatt tacattttca
     ttaaatcgct ctacaagacc actatatttt tctttaactt gcccatgttc tttacttaat
     tttttatatt ctctcgccat atcagtactc atgagatttc taacatgctg ttttaaccta
     tcgttatctc tcgcagcagt cactaagttt ttataatcac gctccgatat aacaacattt
     ttggttggtt tcttttctgt tttcattatt tcttttccca aaccaaacat ggacttttca
     cccgttggca cttcaacact tttcatgtgt cgtttcgctg gtacttctaa atctgattta
     actttatcgc tataagcagt ccattcatct tttttaactg ctaaattttt ttctagaaaa
     tcaatctctt tttccaaagt ttgtttttta aatttagctg tctcaatatg tttacggtca
     gagccacgtt caccacgctt caactcaaaa ccctgttttt tcatatgctc ggggaattta
     tcttgtagcc ataacagttc ttgacgatta aacacatttt ttccttgcag ttttccatca
     cgcataggca caacacctaa atgcatgtga ggggtttgct catcattatg aactgttgca
     taagcaatat tttgcttgcc atatcgttcg gaaaataatt tataactttc ctcaaaaaat
     cgtttttgtt ctcctggatc cagttgctca aaaaaatctc ggtcagatgt tactagcaac
     tcatttacaa gaacagcatc tttcctccgt ttttcttgta cctgtttttt gtgattcaat
     aatttctttg acacgttcgt tgtaatcaat atttttatca tttttcaaat cataattttc
     acgtgttcgc tcatggtcaa tatcatcatt cgttctactt tttcgctctc tttgattatg
     aaattgcatg ccttttagtc cagctgattt cactttttgc attctacaaa ctgcataact
     catatgtaaa tcgctccttt ttaggtggca caaatgtgag gcattttcgc tctttccggc
     gaggctagtt acccttaagt tattggtatg actggtttta agcgcaaaaa aagttgcttt
     ttcgtaccta ttaatgtatc gttttagaaa accgactgta aaaagtacag tcggcattat
     ctcatattat aaaagccagt cattaggcct atctgacaat tcctgaatag agttcataaa
     caatcctgca tgataaccat cacaaacaga atgatgtacc tgtaaagata gcggtaaata
     tattgaatta cctttattaa tgaattttcc tgctgtaata atgggtagaa ggtaattact
     attattattg atatttaagt taaacccagt aaatgaagtc catggaataa tagaaagaga
     aaaagcattt tcaggtatag gtgttttggg aaacaatttc cccgaaccat tatatttctc
     tacatcagaa aggtataaat cataaaactc tttgaagtca ttctttacag gagtccaaat
     accagagaat gttttagata caccatcaaa aattgtataa agtggctcta acttatccca
     ataacctaac tctccgtcgc tattgtaacc agttctaaaa gctgtatttg agtttatcac
     ccttgtcact aagaaaataa atgcagggta aaatttatat ccttcttgtt ttatgtttcg
     gtataaaaca ctaatatcaa tttctgtggt tatactaaaa gtcgtttgtt ggttcaaata
     atgattaaat atctcttttc tcttccaatt gtctaaatca attttattaa agttcatttg
     atatgcctcc taaattttta tctaaagtga atttaggagg cttacttgtc tgctttcttc
     attagaatca atcctttttt aaaagtcaat attactgtaa cataaatata tattttaaaa
     atatcccact ttatccaatt ttcgtttgtt gaactaatgg gtgctttagt tgaagaataa
     agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa ggatttgagc gtagcgaaaa
     atccttttct ttcttatctt gataataagg gtaactattg
//