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ID PBD64 preliminary; circular DNA; SYN; 4780 BP.
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AC X51450; ATCC37104;
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DT 18-APR-1990 (Rel. 5, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Bacillus/Staphylococcus plasmid vector pBD64 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-4780
RC pBD64
RA Minton N.P.;
RT ;
RL Submitted (16-JAN-1990) on tape to the EMBL Data Library by:
RL Minton N.P., Centre for Applied Microbiology and Research,
RL Molecular Genetics Group, Division of Biotechnology PHLS CAMR,
RL Porton Down, Salisbury Wiltshire SP4 0JG, United Kingdom.
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RN [2]
RP 1-4780
RC pBD12 from pUB110 & pC194
RC pBD64 from pBD12
RA Minton N.P., Swinfield T.J., Brehm J.K., Oultram J.D.;
RT "The Gram positive cloning vector pBD64 arose by a 1844 bp
RT deletion of pC194 derived DNA";
RL Nucleic Acids Res. 18:1651-1651(1990).
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RN [3]
RC pBD40 from pBD9
RC pBD63 from pBD6 & pHV11
RC pBD64 from pBD12
RA Gryczan T.J., Shivakumar A.G., Dubnau D.;
RT "Characterization of chimeric plasmid cloning vehicles in Bacillus
RT subtilis";
RL J. Bacteriol. 141:246-253(1980).
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CC pBD64 is a chimeric vector composed of plasmids pUB110 and pC194,
CC with a 1844 bp deletion in the pC194 derived DNA.
CC This vector was isolated as a spontaneous deletion variant of pBD12,
CC a chimeric vector composed of plasmids pUB110 and pC194. The deletion
CC consists of 1844 bp (nt 3803 to 5649) of pBD12, & encompasses
CC nt 1 to 487 & 1552 to 2906 of the pC194 sequence. [2]
CC Medium is 1179 V Y medium.
CC NM (pBD64)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (B.subtilis BD170)(B.subtilis)(Staphylococcus aureus)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBD12 from pUB110 and pC194)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBD12 remove 1887bp, pC194/not cat/4780bp
FT -> pBD64 4780bp"
FT CDS complement(242..1012)
FT /note="ANT Bacillus neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)
FT kanamycin resistance gene (kan) (AA 1-256)"
FT CDS complement(1172..2155)
FT /note="GEN Bacillus repB gene (AA 1-327)"
FT rep_origin complement(2335..2335)
FT /note="ORI Bacillus pUB110"
FT misc_feature 3803..3804
FT /note="site of deleted pC194 DNA"
FT CDS complement(3847..4497)
FT /note="ANT Bacillus chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (Cmr)
FT (AA 1-216)"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT misc_binding 0..0
FT /note="SIT unique XbaI"
FT misc_binding 0..0
FT /note="SIT unique BamHI"
FT misc_binding 0..0
FT /note="SIT unique TacI"
FT misc_binding 0..0
FT /note="SIT unique BglII"
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SQ Sequence 4780 BP; 1399 A; 976 C; 648 G; 1757 T; 0 other;
ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta atgtaaatct
attaccttat tattaattca attcgctcat aattaatcct ttttcttatt acgcaaaatg
gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg ataattacta
atactaggag aagttaataa atacgtaacc aacatgatta acaattatta gaggtcatcg
ttcaaaatgg tatgcgtttt gacacatcca ctatatatcc gtgtcgttct gtccactcct
gaatcccatt ccagaaattc tctagcgatt ccagaagttt ctcagagtcg gaaagttgac
cagacattac gaactggcac agatggtcat aacctgaagg aagatctgat tgcttaactg
cttcagttaa gaccgaagcg ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca
tggcacctgc cattgctacc tgtacagtca aggatggtag aaatgttgtc ggtccttgca
cacgaatatt acgccatttg cctgcatatt caaacagctc ttctacgata agggcacaaa
tcgcatcgtg gaacgtttgg gcttctaccg atttagcagt ttgatacact ttctctaagt
atccacctga atcataaatc ggcaaaatag agaaaaattg accatgtgta agcggccaat
ctgattccac ctgagatgca taatctagta gaatctcttc gctatcaaaa ttcacttcca
ccttccactc accggttgtc cattcatggc tgaactctgc ttcctctgtt gacatgacac
acatcatctc aatatccgaa tagggcccat cagtctgacg accaagagag ccataaacac
caatagcctt aacatcatcc ccatatttat ccaatattcg ttccttaatt tcatgaacaa
tcttcattct ttcttctcta gtcattatta ttggtccatt cactattctc attccctttt
cagataattt tagatttgct tttctaaata agaatatttg gagagcaccg ttcttattca
gctattaata actcgtcttc ctaagcatcc ttcaatcctt ttaataacaa ttatagcatc
taatcttcaa caaactggcc cgtttgttga actactcttt aataaaataa tttttccgtt
cccaattcca cattgcaata atagaaaatc catcttcatc ggctttttcg tcatcatctg
tatgaatcaa atcgccttct tctgtgtcat caaggtttaa ttttttatgt atttctttta
acaaaccacc ataggagatt aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac
gtttcaaatt cttttcttca tcatcggtca taaaatccgt atcctttaca ggatattttg
cagtttcgtc aattgccgat tgtatatccg atttatattt atttttcggt cgaatcattt
gaacttttac atttggatca tagtctaatt tcattgcctt tttccaaaat tgaatccatt
gtttttgatt cacgtagttt tctgtattct taaaataagt tggttccaca cataccaata
catgcatgtg ctgattataa gaattatctt tattatttat tgtcacttcc gttgcacgca
taaaaccaac aagattttta ttaatttttt tatattgcat cattcggcga aatccttgag
ccatatctga caaactctta tttaattctt cgccatcata aacattttta actgttaatg
tgagaaacaa ccaacgaact gttggctttt gtttaataac ttcagcaaca accttttgtg
actgaatgcc atgtttcatt gctctcctcc agttgcacat tggacaaagc ctggatttac
aaaaccacac tcgatacaac tttctttcgc ctgtttcacg attttgttta tactctaata
tttcagcaca atcttttact ctttcagcct ttttaaattc aagaatatgc agaagttcaa
agtaatcaac attagcgatt ttcttttctc tccatggtct cacttttcca ctttttgtct
tgtccactaa aacccttgat ttttcatctg aataaatgct actattagga cacataatat
taaaagaaac ccccatctat ttagttattt gtttagtcac ttataacttt aacagatggg
gtttttctgt gcaaccaatt ttaagggttt tcaatacttt aaaacacata cataccaaca
cttcaacgca cctttcagca actaaaataa aaatgacgtt atttctatat gtatcaagat
aagaaagaac aagttcaaaa ccatcaaaaa aagacacctt ttcaggtgct ttttttattt
tataaactca ttccctgatc tcgacttcgt tcttttttta cctctcggtt atgagttagt
tcaaattcgt tctttttagg ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc
tttaccttgt ctacaaaccc cttaaaaacg tttttaaagg cttttaagcc gtctgtacgt
tccttaagga attccttagt gctttcatag attaaactca catcacgctt taaatcgctt
attttagact ttaaagactt gttttcttca agcaactcat tataatcatt tacattttca
ttaaatcgct ctacaagacc actatatttt tctttaactt gcccatgttc tttacttaat
tttttatatt ctctcgccat atcagtactc atgagatttc taacatgctg ttttaaccta
tcgttatctc tcgcagcagt cactaagttt ttataatcac gctccgatat aacaacattt
ttggttggtt tcttttctgt tttcattatt tcttttccca aaccaaacat ggacttttca
cccgttggca cttcaacact tttcatgtgt cgtttcgctg gtacttctaa atctgattta
actttatcgc tataagcagt ccattcatct tttttaactg ctaaattttt ttctagaaaa
tcaatctctt tttccaaagt ttgtttttta aatttagctg tctcaatatg tttacggtca
gagccacgtt caccacgctt caactcaaaa ccctgttttt tcatatgctc ggggaattta
tcttgtagcc ataacagttc ttgacgatta aacacatttt ttccttgcag ttttccatca
cgcataggca caacacctaa atgcatgtga ggggtttgct catcattatg aactgttgca
taagcaatat tttgcttgcc atatcgttcg gaaaataatt tataactttc ctcaaaaaat
cgtttttgtt ctcctggatc cagttgctca aaaaaatctc ggtcagatgt tactagcaac
tcatttacaa gaacagcatc tttcctccgt ttttcttgta cctgtttttt gtgattcaat
aatttctttg acacgttcgt tgtaatcaat atttttatca tttttcaaat cataattttc
acgtgttcgc tcatggtcaa tatcatcatt cgttctactt tttcgctctc tttgattatg
aaattgcatg ccttttagtc cagctgattt cactttttgc attctacaaa ctgcataact
catatgtaaa tcgctccttt ttaggtggca caaatgtgag gcattttcgc tctttccggc
gaggctagtt acccttaagt tattggtatg actggtttta agcgcaaaaa aagttgcttt
ttcgtaccta ttaatgtatc gttttagaaa accgactgta aaaagtacag tcggcattat
ctcatattat aaaagccagt cattaggcct atctgacaat tcctgaatag agttcataaa
caatcctgca tgataaccat cacaaacaga atgatgtacc tgtaaagata gcggtaaata
tattgaatta cctttattaa tgaattttcc tgctgtaata atgggtagaa ggtaattact
attattattg atatttaagt taaacccagt aaatgaagtc catggaataa tagaaagaga
aaaagcattt tcaggtatag gtgttttggg aaacaatttc cccgaaccat tatatttctc
tacatcagaa aggtataaat cataaaactc tttgaagtca ttctttacag gagtccaaat
accagagaat gttttagata caccatcaaa aattgtataa agtggctcta acttatccca
ataacctaac tctccgtcgc tattgtaacc agttctaaaa gctgtatttg agtttatcac
ccttgtcact aagaaaataa atgcagggta aaatttatat ccttcttgtt ttatgtttcg
gtataaaaca ctaatatcaa tttctgtggt tatactaaaa gtcgtttgtt ggttcaaata
atgattaaat atctcttttc tcttccaatt gtctaaatca attttattaa agttcatttg
atatgcctcc taaattttta tctaaagtga atttaggagg cttacttgtc tgctttcttc
attagaatca atcctttttt aaaagtcaat attactgtaa cataaatata tattttaaaa
atatcccact ttatccaatt ttcgtttgtt gaactaatgg gtgctttagt tgaagaataa
agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa ggatttgagc gtagcgaaaa
atccttttct ttcttatctt gataataagg gtaactattg
//