back Return to this vector's summary.
ID   PBD9       preliminary; circular DNA; SYN; 8276 BP.
XX
AC   ATCC37098;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/Staphylococcus plasmid vector pBD9 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBD6 from pSA0501 & pUB110
RC   pBD7 from B.licheniformis DNA & pUB110
RC   pBD8 from pUB110 & pSA2100
RC   pBD9 from pUB110 & pE194
RC   pBD10, pBD11 from pBD8 & pE194
RC   pBD12 from pUB110 & pCM194
RA   Gryczan T.J., Dubnau D.;
RT   "Construction and properties of chimeric plasmids in Bacillus
RT   subtilis";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1428-1432(1978).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   A general purpose plasmid vector for cloning in Bacillus subtilis.
CC   (ATCC staff)
CC   This plasmid is maintained at a high copy number.
CC   Medium is 1179 V Y medium.
CC   NM (pBD9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BD170)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pE194)
CC   BR (pBD8)(pBD6)(pBD10)(pBD11)(pBD12)(pBD20)(pBD35)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUB110 XbaI 4548bp 465..465, kan gene
FT                   2. pE194 XbaI 3728bp 3480..3480, ery gene
FT                   -> pBD9 8276bp"
FT   -               1..464
FT                   /note="pUB110 1..464 464bp
FT                   XbaI = T^CTAGA"
FT   -               465..4192
FT                   /note="pE194 3480..3728..3479 3728bp
FT                   XbaI = T^CTAGA"
FT   -               4193..8276
FT                   /note="pUB110 465..4548 4084bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-TacI-PstI-BglII-
FT                   BclI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE194"
FT   CDS             0..0
FT                   /note="ANT Bacillus erythromycin resistance gene
FT                   (erm)"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
XX
SQ   Sequence 8276 BP; 2669 A; 1460 C; 1346 G; 2801 T; 0 other;
     gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga
     ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg
     ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata
     ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc
     tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg
     tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg
     cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc
     gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatgaatacg
     gtaaggaaaa tatgctgtat gcgactgtcc atctggatga aagagtccca catatgcact
     ttggttttgt ccctttaaca gaggacggga gattgtctgc aaaagaacag ttaggcaaca
     agaaagactt tactcaatta caagatagat ttaatgagta tgtgaatgag aaaggttatg
     aacttgaaag aggcacgtcc aaagaggtta cagaacgaga acataaagcg atggatcagt
     acaagaaaga tactgtattt cataaacagg aactgcaaga agttaaggat gagttacaga
     aggcaaataa gcagttacag agtggaatag agcatatgag gtctacgaaa ccctttgatt
     atgaaaatga gcgtacaggt ttgttctctg gacgtgaaga gactggtaga aagatattaa
     ctgctgatga atttgaacgc ctgcaagaaa caatctcttc tgcagaacgg attgttgatg
     attacgaaaa tattaagagc acagactatt acacagaaaa tcaagaatta aaaaaacgta
     gagagagttt gaaagaagta gtgaatacat ggaaagaggg gtatcacgaa aaaagtaaag
     aggttaataa attaaagcga gagaatgata gtttgaatga gcagttgaat gtatcagaga
     aatttcaagc tagtacagtg actttatatc gtgctgcgag ggcgaatttc cctgggtttg
     agaaagggtt taataggctt aaagagaaat tctttaatga ttccaaattt gagcgtgtgg
     gacagtttat ggatgttgta caggataatg tccagaaggt cgatagaaag cgtgagaaac
     agcgtacaga cgatttagag atgtagaggt acttttatgc cgagaaaact ttttgcgtgt
     gacagtcctt aaaatatact tagagcgtaa gcgaaagtag tagcgacagc tattaacttt
     cggttgcaaa gctctaggat ttttaatgga cgcagcgcat cacacgcaaa aaggaaattg
     gaataaatgc gaaatttgag atgttaatta aagacctttt tgaggtcttt ttttcttaga
     tttttggggt tatttagggg agaaaacata ggggggtact acgacctccc ccctaggtgt
     ccattgtcca ttgtccaaac aaataaataa atattgggtt tttaatgtta aaaggttgtt
     ttttatgtta aagtgaaaaa aacagatgtt gggaggtaca gtgatggttg tagatagaaa
     agaagagaaa aaagttgctg ttactttaag acttacaaca gaagaaaatg agatattaaa
     tagaatcaaa gaaaaatata atattagcaa atcagatgca accggtattc taataaaaaa
     atatgcaaag gaggaatacg gtgcatttta aacaaaaaaa gatagacagc actggcatgc
     tgcctatcta tgactaaatt ttgttaagtg tattagcacc gttattatat catgagcgaa
     aatgtaataa aagaaactga aaacaagaaa aattcaagag gacgtaattg gacatttgtt
     ttatatccag aatcagcaaa agccgagtgg ttagagtatt taaaagagtt acacattcaa
     tttgtagtgt ctccattaca tgatagggat actgatacag aaggtaggat gaaaaaagag
     cattatcata ttctagtgat gtatgagggt aataaatctt atgaacagat aaaaataatt
     aacagaagaa ttgaatgcga ctattccgca gattgcagga agtgtgaaag gtcttgtgag
     atatatgctt cacatggacg atcctaataa atttaaatat caaaaagaag atatgatagt
     ttatggcggt gtagatgttg atgaattatt aaagaaaaca acaacagata gatataaatt
     aattaaagaa atgattgagt ttattgatga acaaggaatc gtagaattta agagtttaat
     ggattatgca atgaagttta aatttgatga ttggttcccg cttttatgtg ataactcggc
     gtatgttatt caagaatata taaaatcaaa tcggtataaa tctgaccgat agattttgaa
     tttaggtgtc acaagacact cttttttcgc accagcgaaa actggtttaa gccgactgcg
     caaaagacat aatcgattca caaaaaatag gcacacgaaa aacaagttaa gggatgcagt
     ttatgcatcc cttaacttac ttattaaata atttatagct attgaaaaga gataagaatt
     gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt
     gattttttgt aaatattttc ttgtattctt tgttaaccca tttcataacg aaataattat
     acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg ataagtgagc
     tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat atagaaatat
     caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat cttttagcaa
     acccgtattc cacgattaaa taaatctcat tagctatact atcaaaaaca attttgcgta
     ttatatccgt acttatgtta taaggtatat taccatatat tttataggat tggtttttag
     gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga tcaacaagtt
     tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg aaattacacc
     tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag atattatcat
     gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa gtaataaagt
     tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg gttatatgaa
     ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat aatgaactgt
     gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa ttagtataat
     tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt atactgcaat
     cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt ttcttgtaaa
     aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt taacgactta
     attacgaagt aaataagtct agtgtgttag actttatgaa atctatatac gtttatatat
     atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca gagttaaagg
     atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa actataataa
     taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg cacaaaatat
     aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga aacgtaaaat
     tcggtcagat gcaattcgac atgtggacgg actggttaca agtgataaag atttctttga
     tgatttaagc ggagaagaaa tagaacgatt ttttaaagat agcttggagt ttctagaaaa
     tcaatctctt tttccaaagt ttgtttttta aatttagctg tctcaatatg tttacggtca
     gagccacgtt caccacgctt caactcaaaa ccctgttttt tcatatgctc ggggaattta
     tcttgtagcc ataacagttc ttgacgatta aacacatttt ttccttgcag ttttccatca
     cgcataggca caacacctaa atgcatgtga ggggtttgct catcattatg aactgttgca
     taagcaatat tttgcttgcc atatcgttcg gaaaataatt tataactttc ctcaaaaaat
     cgtttttgtt ctcctggatc cagttgctca aaaaaatctc ggtcagatgt tactagcaac
     tcatttacaa gaacagcatc tttcctccgt ttttcttgta cctgtttttt gtgattcaat
     aatttctttg acacgttcgt tgtaatcaat atttttatca tttttcaaat cataattttc
     acgtgttcgc tcatggtcaa tatcatcatt cgttctactt tttcgctctc tttgattatg
     aaattgcatg ccttttagtc cagctgattt cactttttgc attctacaaa ctgcataact
     catatgtaaa tcgctccttt ttaggtggca caaatgtgag gcattttcgc tctttccggc
     aaccacttcc aagtaaagta taacacacta tactttatat tcataaagtg tgtgctctgc
     gaggctgtcg gcagtgccga ccaaaaccat aaaaccttta agacctttct tttttttacg
     agaaaaaaga aacaaaaaaa cctgccctct gccacctcag caaagggggg ttttgctctc
     gtgctcgttt aaaaatcagc aagggacagg tagtattttt tgagaagatc actcaaaaaa
     tctccacctt taaacccttg ccaattttta ttttgtccgt tttgtctagc ttaccgaaag
     ccagactcag caagaataaa atttttattg tctttcggtt ttctagtgta acggacaaaa
     ccactcaaaa taaaaaagat acaagagagg tctctcgtat cttttattca gcaatcgcgc
     ccgattgctg aacagattaa taatagattt tagcttttta tttgttgaaa aaagctaatc
     aaattgttgt cgggatcaat tactgcaaag tctcgttcat cccaccactg atcttttaat
     gatgtattgg ggtgcaaaat gcccaaaggc ttaatatgtt gatataattc atcaattccc
     tctacttcaa tgcggcaact agcagtacca gcaataaacg actccgcacc tgtacaaacc
     ggtgaatcat tactacgaga gcgccagctt catcacttgc ctcccataga tgaatccgaa
     cctcattaca cattagaact gcgaatccat cttcatggtg aaccaaagtg aaacctagtt
     tatcgcaata aaaacctata ctctttttaa tatccccgac tggcaatgcc gggatagact
     gtaacattct cacgcataaa atcccctttc attttctaat gtaaatctat taccttatta
     ttaattcaat tcgctcataa ttaatccttt ttcttattac gcaaaatggc ccgatttaag
     cacacccttt attccgttaa tgcgccatga cagccatgat aattactaat actaggagaa
     gttaataaat acgtaaccaa catgattaac aattattaga ggtcatcgtt caaaatggta
     tgcgttttga cacatccact atatatccgt gtcgttctgt ccactcctga atcccattcc
     agaaattctc tagcgattcc agaagtttct cagagtcgga aagttgacca gacattacga
     actggcacag atggtcataa cctgaaggaa gatctgattg cttaactgct tcagttaaga
     ccgaagcgct cgtcgtataa cagatgcgat gatgcagacc aatcaacatg gcacctgcca
     ttgctacctg tacagtcaag gatggtagaa atgttgtcgg tccttgcaca cgaatattac
     gccatttgcc tgcatattca aacagctctt ctacgataag ggcacaaatc gcatcgtgga
     acgtttgggc ttctaccgat ttagcagttt gatacacttt ctctaagtat ccacctgaat
     cataaatcgg caaaatagag aaaaattgac catgtgtaag cggccaatct gattccacct
     gagatgcata atctagtaga atctcttcgc tatcaaaatt cacttccacc ttccactcac
     cggttgtcca ttcatggctg aactctgctt cctctgttga catgacacac atcatctcaa
     tatccgaata gggcccatca gtctgacgac caagagagcc ataaacacca atagccttaa
     catcatcccc atatttatcc aatattcgtt ccttaatttc atgaacaatc ttcattcttt
     cttctctagt cattattatt ggtccattca ctattctcat tcccttttca gataatttta
     gatttgcttt tctaaataag aatatttgga gagcaccgtt cttattcagc tattaataac
     tcgtcttcct aagcatcctt caatcctttt aataacaatt atagcatcta atcttcaaca
     aactggcccg tttgttgaac tactctttaa taaaataatt tttccgttcc caattccaca
     ttgcaataat agaaaatcca tcttcatcgg ctttttcgtc atcatctgta tgaatcaaat
     cgccttcttc tgtgtcatca aggtttaatt ttttatgtat ttcttttaac aaaccaccat
     aggagattaa ccttttacgg tgtaaacctt cctccaaatc agacaaacgt ttcaaattct
     tttcttcatc atcggtcata aaatccgtat cctttacagg atattttgca gtttcgtcaa
     ttgccgattg tatatccgat ttatatttat ttttcggtcg aatcatttga acttttacat
     ttggatcata gtctaatttc attgcctttt tccaaaattg aatccattgt ttttgattca
     cgtagttttc tgtattctta aaataagttg gttccacaca taccaataca tgcatgtgct
     gattataaga attatcttta ttatttattg tcacttccgt tgcacgcata aaaccaacaa
     gatttttatt aattttttta tattgcatca ttcggcgaaa tccttgagcc atatctgaca
     aactcttatt taattcttcg ccatcataaa catttttaac tgttaatgtg agaaacaacc
     aacgaactgt tggcttttgt ttaataactt cagcaacaac cttttgtgac tgaatgccat
     gtttcattgc tctcctccag ttgcacattg gacaaagcct ggatttacaa aaccacactc
     gatacaactt tctttcgcct gtttcacgat tttgtttata ctctaatatt tcagcacaat
     cttttactct ttcagccttt ttaaattcaa gaatatgcag aagttcaaag taatcaacat
     tagcgatttt cttttctctc catggtctca cttttccact ttttgtcttg tccactaaaa
     cccttgattt ttcatctgaa taaatgctac tattaggaca cataatatta aaagaaaccc
     ccatctattt agttatttgt ttagtcactt ataactttaa cagatggggt ttttctgtgc
     aaccaatttt aagggttttc aatactttaa aacacataca taccaacact tcaacgcacc
     tttcagcaac taaaataaaa atgacgttat ttctatatgt atcaagataa gaaagaacaa
     gttcaaaacc atcaaaaaaa gacacctttt caggtgcttt ttttatttta taaactcatt
     ccctgatctc gacttcgttc tttttttacc tctcggttat gagttagttc aaattcgttc
     tttttaggtt ctaaatcgtg tttttcttgg aattgtgctg ttttatcctt taccttgtct
     acaaacccct taaaaacgtt tttaaaggct tttaagccgt ctgtacgttc cttaag
//