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ID   PBE20      preliminary; circular DNA; SYN; 5771 BP.
XX
AC   IG5068;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pBE20 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBE20 from pTZ18R & pC194
RC   pBE60 from pBR322 & pUB110 & M13
RC   pBE30 from pBE series
RC   pBE90 from pBE series
RC   pBE91 from pBE series
RC   pBE597 from pBE series
RA   Nagarajan V., Albertson H., Chen M., Ribbe J.;
RT   "Modular expression and secretion vectors for Bacillus subtilis";
RL   Gene 114:121-126(1992).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS. not original sequence.
CC   NM (pBE20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pTZ18R)(pC194)
CC   BR (pBE60)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ18R HindIII 2861bp 163..163
FT                   2. pC194 HindIII 2910bp 2436..2436
FT                   -> pBE20 5771bp"
FT   -               1..162
FT                   /note="pTZ18R 1..162 162bp
FT                   HindIII = A^AGCTT"
FT   -               163..3072
FT                   /note="pC194 2436..2910..2435 2910bp
FT                   HindIII = A^AGCTT"
FT   -               3073..5771
FT                   /note="pTZ18R 163..2861 2699bp"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pC194"
FT   CDS             0..0
FT                   /note="ANT Bacillus cat gene"
FT   misc_feature    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI phage f1 ori"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="MCS EcoRI-SstI-XmaI/KpnI-SmaI-BamHI-XbaI-
FT                   AccI/HincII/SalI-PstI-SphI-HindIII"
FT   misc_feature    0..0
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 5771 BP; 1664 A; 1216 C; 1065 G; 1826 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctttta aaaagcaaat
     atgagccaaa taaatatatt ctaattctac aaacaaaaat ttgagcaaat tcagtgtcga
     ttttttaaga cactgcccag ttacatgcaa attaaaattt tcatgatttt ttatagttcc
     taacagggtt aaaatttgta taacgaaagt ataatgttta tataacgtta gtataataaa
     gcattttaac attatacttt tgataatcgt ttatcgtcgt catcacaata acttttaaaa
     tactcgtgca taattcacgc tgacctccca ataactacat ggtgttatcg ggaggtcagc
     tgttagcact tatattttgt tattgttctt cctcgatttc gtctatcatt ttgtgattaa
     tttctctttt ttcttgttct gttaagtcat aaagttcact agctaaatac tctttttgtt
     tccaaatata aaaaatttga tagatatatt acggttggat caatttcttt taagtaatct
     aaatccccat tttttaattt ctttttagcc tctttaaata atcctgaata aactaatacc
     tgtttacctt taagtgattt ataaaatgca tcaaagactt tttgatttat ttattaaata
     atcactatct ttaccagaat acttagccat ttcatataat tctttattat tattttgtct
     tattttttga acttgaactt gtgttatttc tgaaatgccc gttacatcac gccataaatc
     taaccattct tgttggctaa tataatatct tttatctgtg aaatacgatt tatttactgc
     aattaacaca tgaaaatgag gattataatc atctcttttt ttattatatg taatctctaa
     cttacgaaca tatcccttta taacactacc tacttttttt ctctttataa gttttctaaa
     agaattatta taacgtttta tttcattttc taattcatca ctcattacat taggtgtagt
     caaagttaaa aagataaact cctttttctc ttgctgctta atatattgca tcatcaaaga
     taaacccaat gcatcttttc tagcttttct ccaagcacag acaggacaaa atcgattttt
     acaagaatta gctttatata atttctgttt ttctaaagtt ttatcagcta caaaagacag
     aaatgtattg caatcttcaa ctaaatccat ttgattctct ccaatatgac gtttaataaa
     tttctgaaat acttgatttc tttgtttttt ctcagtatac ttttccatgt tataacacat
     aaaaacaact tagttttcac aaactatgac aataaaaaaa gttgcttttt cccctttcta
     tgtatgtttt ttactagtca tttaaaacga tacattaata ggtacgaaaa agcaactttt
     tttgcgctta aaaccagtca taccaataac ttaagggtaa ctagcctcgc cggcaatagt
     tacccttatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa tcctttaaaa
     aaacacaaaa gaccacattt tttaatgtgg tctttattct tcaactaaag cacccattag
     ttcaacaaac gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt
     aatattgact tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta
     aattcacttt agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta
     gacaattgga agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt
     ataaccacag aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa
     ttttaccctg catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga
     actggttaca atagcgacgg agagttaggt tattgggata agttagagcc actttataca
     atttttgatg gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc
     aaagagtttt atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg
     tttcccaaaa cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca
     tttactgggt ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca
     gcaggaaaat tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat
     cattctgttt gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca
     gataggccta atgactggct tttataatat gagataatgc cgactgtact ttttacagtc
     ggttttctaa tgtcactaac ctgccccgtt agttgaagaa ggtttttata ttacagctcc
     agatccatat ccttcttttt ctgaaccgac ttctcctttt tcgcttcttt attccaattg
     ctttattgac gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct
     taatagctca cgctatgccg acattcgtct gcaagtttag ttaagggttc ttctcaacgc
     acaataaatt ttctcggcat aaatgcgtgg tctaattttt atttttaata accttgatag
     caaaaaatgc cattccaata caaaaccaca tacctataat cgataaccac ataacagtca
     taaaaccact cctttttaac aaactttatc acaagaaata tttaaatttt aaatgccttt
     attttgaatt ttaaggggca ttttaaagat ttaggggtaa atcatatagt tttatgccta
     aaaacctaca gaagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
     tcgaattccc tatagtgagt cgtattaaat tcgtaatcat ggtcatagct gtttcctgtg
     tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaaac
     gtttacaatt t
//