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ID   PBGS9      preliminary; circular DNA; SYN; 3665 BP.
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AC   M18327; ATCC37452;
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DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBGS9 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPH120 from pHP34 & pLG338
RC   pPH121 from pPH120
RC   pPH124 from pPH121
RC   pPH125 from pPH124 & pPH120
RC   pPH126 from pPH125
RC   pPH114 from pPH125
RC   pBS105 from pPH114
RC   pteH8 from pPH125 & pUC8
RC   pBS8 from pteH8 & pBS105
RC   pBS8+, pBS8- from pBS8 & pEMBL8+
RC   pteH9 from pPH125 & pUC9
RC   pBS9 from pteH9 & pBS105
RC   pBS9+, pBS9- from pBS9 & pEMBL9+
RC   [pBG131 from pBS series]
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT   pUC9, pEMBL8 and pEMBL9";
RL   Gene 41:337-342(1986).
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RN   [2]
RP   1-3665
RC   pBGS9 from pUC9 & kan gene
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   ;
RL   Unpublished (1988).
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RN   [3]
RP   1-3665
RC   pBGS9
RA   Spratt B.G.;
RT   ;
RL   Submitted (26-FEB-1988) to GenBank on computer-readable media by:
RL   Spratt B.G., Univ of Sussex, Falmer, Brighton BNI 9QG U.K.
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CC   The duplicate HindIII, SmaI and AccI sites have been removed.
CC   Kanamycin-resistant analog of pUC9.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pBGS9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (expression)(fusion)
CC   SE (color blue/white)
CC   PA (Tn903)(pBR322)
CC   BR (pUC8)(pUC9)(pEMBL8)(pEMBL9)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pPH125 SmaI 5300bp, pHP34 4299..4299
FT                   fill in
FT                   remove EcoRI-BamHI 1300bp, pHP34 4292..5300..306
FT                   \ 4000bp
FT                   2. E. coli EcoRI-BamHI 2600bp, pbpB gene
FT                   -> pPH114 6600bp [unique HindIII in kan]
FT                   1. pPH114 HindIII 6600bp
FT                   mutagenesis of ends
FT                   -> pBS105 6600bp [no SmaI, no HindIII]
FT                   1. pPH125 remove PvuII-EcoRI 2293bp 1999..4292,
FT                   \ tet gene/3000bp
FT                   S1 nuclease
FT                   2. pUC9 HaeII-HaeII 794bp 2300..2665..429, lacPOZ'
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pteH9 3660bp
FT                   1. pteH9 remove MluI-MluI 800bp, kan gene
FT                   2. pBS105 MluI-MluI 800bp,
FT                   \ kan gene with no SmaI or HindIII
FT                   -> pBGS9 3665bp"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacZ gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-BamHI-HincII-AccI-SalI-
FT                   PstI-HindIII"
XX
SQ   Sequence 3665 BP; 912 A; 882 C; 941 G; 928 T; 2 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccggggat ccgtcgacct
     gcagccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgccccctgt aatccgggca
     gcgcaacgga acattcatca gtgtaaaaat ggaatcaata aagccctgcg cagcgcgcag
     ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcg
     ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc
     atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt
     ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat
     ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa
     tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
     gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     tgatgatgca tggttactca ccactgcgat cccngggaaa acagcattcc aggtattaga
     agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     tggctggcct gttgaacaag tctggaaaga aatgcataan cttttgccat tctcaccgga
     ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ctattctgac
     cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct gcgcgctgcg
     cagggcttta ttgattccat ttttacactg atgaatgttc cgttgcgctg cccggattac
     agggg
//