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ID PBGS9 preliminary; circular DNA; SYN; 3665 BP.
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AC M18327; ATCC37452;
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DT 16-JUL-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pBGS9 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pPH120 from pHP34 & pLG338
RC pPH121 from pPH120
RC pPH124 from pPH121
RC pPH125 from pPH124 & pPH120
RC pPH126 from pPH125
RC pPH114 from pPH125
RC pBS105 from pPH114
RC pteH8 from pPH125 & pUC8
RC pBS8 from pteH8 & pBS105
RC pBS8+, pBS8- from pBS8 & pEMBL8+
RC pteH9 from pPH125 & pUC9
RC pBS9 from pteH9 & pBS105
RC pBS9+, pBS9- from pBS9 & pEMBL9+
RC [pBG131 from pBS series]
RA Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT pUC9, pEMBL8 and pEMBL9";
RL Gene 41:337-342(1986).
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RN [2]
RP 1-3665
RC pBGS9 from pUC9 & kan gene
RA Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT ;
RL Unpublished (1988).
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RN [3]
RP 1-3665
RC pBGS9
RA Spratt B.G.;
RT ;
RL Submitted (26-FEB-1988) to GenBank on computer-readable media by:
RL Spratt B.G., Univ of Sussex, Falmer, Brighton BNI 9QG U.K.
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CC The duplicate HindIII, SmaI and AccI sites have been removed.
CC Kanamycin-resistant analog of pUC9.
CC Medium is 1236 LB plus kanamycin.
CC NM (pBGS9)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli JM101)(E.coli)
CC CP ()
CC FN (expression)(fusion)
CC SE (color blue/white)
CC PA (Tn903)(pBR322)
CC BR (pUC8)(pUC9)(pEMBL8)(pEMBL9)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pPH125 SmaI 5300bp, pHP34 4299..4299
FT fill in
FT remove EcoRI-BamHI 1300bp, pHP34 4292..5300..306
FT \ 4000bp
FT 2. E. coli EcoRI-BamHI 2600bp, pbpB gene
FT -> pPH114 6600bp [unique HindIII in kan]
FT 1. pPH114 HindIII 6600bp
FT mutagenesis of ends
FT -> pBS105 6600bp [no SmaI, no HindIII]
FT 1. pPH125 remove PvuII-EcoRI 2293bp 1999..4292,
FT \ tet gene/3000bp
FT S1 nuclease
FT 2. pUC9 HaeII-HaeII 794bp 2300..2665..429, lacPOZ'
FT T4 DNA polymerase:T4 DNA polymerase
FT -> pteH9 3660bp
FT 1. pteH9 remove MluI-MluI 800bp, kan gene
FT 2. pBS105 MluI-MluI 800bp,
FT \ kan gene with no SmaI or HindIII
FT -> pBGS9 3665bp"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)
FT kanamycin resistance gene (kan)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli lacZ gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_binding 0..0
FT /note="MCS unique EcoRI-SmaI-BamHI-HincII-AccI-SalI-
FT PstI-HindIII"
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SQ Sequence 3665 BP; 912 A; 882 C; 941 G; 928 T; 2 other;
cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccggggat ccgtcgacct
gcagccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgccccctgt aatccgggca
gcgcaacgga acattcatca gtgtaaaaat ggaatcaata aagccctgcg cagcgcgcag
ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcg
ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc
atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt
ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat
ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa
tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
tgatgatgca tggttactca ccactgcgat cccngggaaa acagcattcc aggtattaga
agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
tggctggcct gttgaacaag tctggaaaga aatgcataan cttttgccat tctcaccgga
ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ctattctgac
cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct gcgcgctgcg
cagggcttta ttgattccat ttttacactg atgaatgttc cgttgcgctg cccggattac
agggg
//