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ID   PBGS9P     preliminary; circular DNA; SYN; 4357 BP.
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AC   M18328; ATCC37453;
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DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBGS9+ - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pPH120 from pHP34 & pLG338
RC   pPH121 from pPH120
RC   pPH124 from pPH121
RC   pPH125 from pPH124 & pPH120
RC   pPH126 from pPH125
RC   pPH114 from pPH125
RC   pBS105 from pPH114
RC   pteH8 from pPH125 & pUC8
RC   pBS8 from pteH8 & pBS105
RC   pBS8+, pBS8- from pBS8 & pEMBL8+
RC   pteH9 from pPH125 & pUC9
RC   pBS9 from pteH9 & pBS105
RC   pBS9+, pBS9- from pBS9 & pEMBL9+
RC   [pBG131 from pBS series]
RA   Spratt B.G., Hedge P.J., Te Heesen S., Eldelman A., Broome-Smith J.K.;
RT   "Kanamycin-resistant vectors that are analogues of plasmids pUC8,
RT   pUC9, pEMBL8 and pEMBL9";
RL   Gene 41:337-342(1986).
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RN   [2]
RP   1-4357
RC   pBGS9+ from pEMBL9+ & kan gene
RA   Spratt B.G.;
RT   ;
RL   Submitted (26-FEB-1988) to GenBank on computer-readable media by:
RL   Spratt B.G., Univ of Sussex, Falmer, Brighton BNI 9QG U.K.
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CC   The duplicate HindIII, SmaI and AccI sites have been removed.
CC   Kanamycin-resistant analog of EMBL9.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pBGS9+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)
CC   CP ()
CC   FN (expression)(fusion)
CC   SE (color blue/white)
CC   PA (Tn903)(pBR322)
CC   BR (pUC8)(pUC9)(pEMBL8)(pEMBL9)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBGS9 remove AhaIII/DraI-AhaIII/DraI 19bp
FT                   \ 1589..1608, 3646bp
FT                   2. pEMBL9+ SspI-SspI 711bp 914..1625, f1 ori
FT                   -> pBGS9+ 4357bp"
FT   misc_binding    0..0
FT                   /note="MCS unique HindIII-PstI-SalI-AccI-HincI-BamHI-
FT                   SmaI-EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacZ gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="SIT unique SspI"
FT   rep_origin      1589..2299
FT                   /note="ORI bacteriophage f1 intergenic region"
XX
SQ   Sequence 4357 BP; 1116 A; 1038 C; 1120 G; 1081 T; 2 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccggggat ccgtcgacct
     gcagccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttat tttgttaaaa ttcgcgttaa atttttgtta
     aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtact atggttgctt
     tgacgagcac gtataacgtg ctttcctcgt tagaatcaga gcgggagcta aacaggaggc
     cgattaaagg gattttagac aggaacggta cgccagaatc ctgagaagtg tttttataat
     cagtgaggcc accgagcaaa agagtctgtc catcacgcaa attaaccgtt gtcgcaatac
     ttctttgatt agtaataaca tcacttgcct gagtagaaga actcaaacta tcggccttgc
     tggtaatatc cagaacaata aatcaatcta aagtatatat gagtaaactt ggtctgacag
     ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     cgttgttgcc attgccccct gtaatccggg cagcgcaacg gaacattcat cagtgtaaaa
     atggaatcaa taaagccctg cgcagcgcgc agggtcagcc tgaatacgcg tttaatgacc
     agcacagtcg tgatggcaag gtcagaatag cgctgaggtc tgcctcgtga agaaggtgtt
     gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg
     ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg
     gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga
     tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
     aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
     gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     atcccnggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     gaaatgcata ancttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     cttcacgagg cagacctcag cgctattctg accttgccat cacgactgtg ctggtcatta
     aacgcgtatt caggctgacc ctgcgcgctg cgcagggctt tattgattcc atttttacac
     tgatgaatgt tccgttgcgc tgcccggatt acagggg
//