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ID   PBHA1      preliminary; circular DNA; SYN; 7336 BP.
XX
AC   A13198; A05160;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pBHA-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7336
RC   pBHA-1 from pBR322
RA   Slijkhuis H., Selten G.C.M., Smaal E.B.;
RT   "Process for the biochemical oxidation of steroids and genetically
RT   engineered cells to be used therefor";
RL   Unpublished (1986).
RL   European Patent No. 0340878-A 19 dated 08-NOV-1989.
RL   GIST-BROCADES N.V.; ROUSSEL-UCLAF.
XX
RN   [2]
RP   1-7336
RC   pBHA-1 from pBR322
RA   Slijkhuis H., Selten G.C.M., Smaal E.B.;
RT   "Process for the biochemical oxidation of steroids and genetically
RT   engineered cells to be used therefor";
RL   Unpublished (1986).
RL   European Patent No. 8804691-A 20 dated 30-JUN-1988.
RL   GIST-BROCADES N.V.; ROUSSEL-UCLAF.
XX
CC   NCBI gi: 491528
CC   NCBI gi: 412615
CC   NM (pBHA-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pBHA-1 7336bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7336 BP; 2182 A; 1433 C; 1659 G; 2062 T; 0 other;
     aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
     ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
     gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
     attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
     atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
     ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
     ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
     cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
     catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
     tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt
     tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cgggagagct cgatatcgca
     tgcggtacct ctagaagaag cttggagaca aggtaaagga taaaacagca caattccaag
     aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac cgagaggtaa
     aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc acctgaaaag
     gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatacata tagaaataac
     gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa
     gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa gttataagtg
     actaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc taatagtagc
     atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg aaaagtgaga
     ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca tattcttgaa
     tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa acaaaatcgt
     gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct ttgtccaatg
     tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt tgctgaagtt
     attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa aaatgtttat
     gatggcgaag aattaaataa gagtttgtca gatatggctc aaggatttcg ccgaatgatg
     caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga agtgacaata
     aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt ggaaccaact
     tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag
     gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa
     tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt aaaggatacg
     gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt
     ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca taaaaaatta
     aaccttgatg acacagaaga aggcgatttg attcatacag atgatgacga aaaagccgat
     gaagatggat tttctattat tgcaatgtgg aattgggaac ggaaaaatta ttttattaaa
     gagtagttca acaaacgggc cagtttgttg aagattagat gctataattg ttattaaaag
     gattgaagga tgcttaggaa gacgagttat taatagctga ataagaacgg tgctctccaa
     atattcttat ttagaaaagc aaatctaaaa ttatctgaaa agggaatgag aatagtgaat
     ggaccaataa taatgactag agaagaaaga atgaagattg ttcatgaaat taaggaacga
     atattggata aatatgggga tgatgttaag gctattggtg tttatggctc tcttggtcgt
     cagactgatg ggccctattc ggatattgag atgatgtgtg tcatgtcaac agaggaagca
     gagttcagcc atgaatggac aaccggtgag tggaaggtgg aagtgaattt tgatagcgaa
     gagattctac tagattatgc atctcaggtg gaatcagatt ggccgcttac acatggtcaa
     tttttctcta ttttgccgat ttatgattca ggtggatact tagagaaagt gtatcaaact
     gctaaatcgg tagaagccca aacgttccac gatgcgattt gtgcccttat cgtagaagag
     ctgtttgaat atgcaggcaa atggcgtaat attcgtgtgc aaggaccgac aacatttcta
     ccatccttga ctgtacaggt agcaatggca ggtgccatgt tgattggtct gcatcatcgc
     atctgttata cgacgagcgc ttcggtctta actgaagcag ttaagcaatc agatcttcct
     tcaggttatg accatctgtg ccagttcgta atgtctggtc aactttccga ctctgagaaa
     cttctggaat cgctagagaa tttctggaat gggattcagg agtggacaga acgacacgga
     tatatagtgg atgtgtcaaa acgcatacca ttttgaacga tgacctctaa taattgttaa
     tcatgttggt tacgtattta ttaacttctc ctagtattag taattatcat ggctgtcatg
     gcgcattaac ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat aagaaaaagg
     attaattatg agcgaattga attaataata aggtaataga tttacattag aaaatgaaag
     gggattttat gcgtgagaat gttacagtct atcccggcat tgccagtcgg ggatattaaa
     aagagtatag gtttttattg cgataaacta ggtttcactt tggttcacca tgaagatgga
     ttcgcagttc taatgtgtaa tgaggttcgg attcatctat gggaggcaag tgatgaaggc
     tggcgctctc gtagtaatga ttcaccggtt tgtacaggtg cggagtcgtt tattgctggt
     actgctagtt gccgcattga agtagaggga attgatgaat tatatcaaca tattaagcct
     ttgggcattt tgcaccccaa tacatcatta aaagatcagt ggtgggatga acgagacttt
     gcagtaattg atcccgacaa caatttgatt agcttttttc aacaaataaa aagctaaaat
     ctattattaa tctgttcagc aatcgggcgc gattgctgaa taaaagatac gagagacctc
     tcttgtatct tttttatttt gagtggtttt gtccgttaca ctagaaaacc gaaagacaat
     aaaaatttta ttcttgctga gtctggcttt cggtaagcta gacaaaacgg acaaaataaa
     aattggcaag ggtttaaagg tggagatttt ttgagtgatc ttctcaaaaa atactacctg
     tcccttgctg atttttaaac gagcacgaga gcaaaacccc cctttgctga ggtggcagag
     ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg
     ttttggtcgg cactgccgac agcctcgcag gacacacact ttatgaatat aaagtatagt
     gtgttatact ttacttggaa gtggttgccg gaaagagcga aaatgcctca catttgtgcc
     acctaaaaag gagcgattta catatgagtt atgcagtttg tagaatgcaa aaagtgaaat
     cagggggatc ctctagagtc gagctcaagc tagcttggta cgtaccagat ctgagatcac
     gcgttctaga ggtcga
//