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ID   PBI101     preliminary; DNA; SYN; 5350 BP.
XX
AC   U12639; IG1034; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Higher plants/E.coli plasmid vector pBI101 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RC   pBI121 from pBI101.1 & CMV 35S promoter
RC   pBI131 from pBI101.1 & tobacco rbcS promoter
RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile gene
RT   fusion marker in higher plants";
RL   EMBO J. 6:3901-3907(1987).
XX
RN   [2]
RP   1-5350
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RA   Wei W., Lindsey K.;
RT   "T-DNA sequence of the GUS gene fusion vector pBI101";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-5350
RC   pBI101.1
RA   Wei W.;
RT   ;
RL   Submitted (22-JUL-1994) by:
RL   Wei W., Botany, University of Leicester, University Road,
RL   Leicester, LE1 7RH, UK.
XX
CC   NM (pBI101)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(plant)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pBIN19)
CC   BR (pBI101.2)(pBI101.3)
CC   OF (pBI121)(pBI221)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBIN19 EcoRI 11777bp 6773..6773, MCS
FT                   2. pRAJ260 EcoRI-EcoRI, GUS gene
FT                   -> pBI101 5350bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO Agrobacterium Ti plasmid nopaline synthase"
FT   CDS             386..1180
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII); neomycin resistance gene (neo)
FT                   E. coli kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT   misc_binding    2498..2538
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             2552..4360
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT SnoBI"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   3'UTR           4431..4683
FT                   /note="TER 3'UTR of the nopaline synthase gene"
FT   repeat_unit     5326..5350
FT                   /note="the left border repeat"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 5350 BP; 1273 A; 1345 C; 1420 G; 1312 T; 0 other;
     gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
     cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
     ctctagagga tccccgggtg gtcagtccct tatgttacgt cctgtagaaa ccccaacccg
     tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
     tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag
     ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca
     gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
     ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
     ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
     caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
     cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat
     ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
     gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
     tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
     tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
     tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg
     catccggtca gtggcagtga agggccaaca gttcctgatt aaccacaaac cgttctactt
     tactggcttt ggtcgtcatg aagatgcgga cttacgtggc aaaggattcg ataacgtgct
     gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca
     ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
     tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
     gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca
     ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
     tgccaacgaa ccggataccc gtccgcaagt gcacgggaat atttcgccac tggcggaagc
     aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     gaacttcggt gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc
     accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc gatcgttcaa
     acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     atcgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt
     cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat
     tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat
     taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     tatcctgcca
//