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ID   PBI1012    preliminary; DNA; SYN; 5351 BP.
XX
AC   U12668; IG1035; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBI101.2 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RC   pBI121 from pBI101.1 & CMV 35S promoter
RC   pBI131 from pBI101.1 & tobacco rbcS promoter
RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile gene
RT   fusion marker in higher plants";
RL   EMBO J. 6:3901-3907(1987).
XX
RN   [2]
RP   1-5351
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RA   Wei W., Lindsey K.;
RT   "T-DNA sequence of the GUS gene fusion vector pBI101.2";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-5351
RC   pBI101.2
RA   Wei W.;
RT   ;
RL   Submitted (22-JUL-1994) by:
RL   Wei W., Botany, University of Leicester, University Road,
RL   Leicester, LE1 7RH, UK.
XX
CC   NCBI gi: 529334
CC   NM (pBI101.2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(plant)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pBIN19)
CC   BR (pBI101.1)(pBI101.3)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBIN19 EcoRI 11777bp 6773..6773, MCS
FT                   2. pRAJ260 EcoRI-EcoRI, GUS gene
FT                   -> pBI101.2 5351bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO Agrobacterium Ti plasmid nopaline synthase"
FT   CDS             386..1180
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII); neomycin resistance gene (neo)
FT                   E. coli kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT   misc_binding    2498..2538
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             2553..4361
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT SnoBI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   3'UTR           4432..4684
FT                   /note="TER 3'UTR of the nopaline synthase gene"
FT   repeat_unit     5327..5351
FT                   /note="the left border repeat"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 5351 BP; 1274 A; 1345 C; 1420 G; 1312 T; 0 other;
     gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
     cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
     ctctagagga tccccgggta ggtcagtccc ttatgttacg tcctgtagaa accccaaccc
     gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa
     ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca
     gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac gtctggtatc
     agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg
     cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg
     gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta
     tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
     ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa
     tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
     tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
     gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
     ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
     atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg
     gcatccggtc agtggcagtg aagggccaac agttcctgat taaccacaaa ccgttctact
     ttactggctt tggtcgtcat gaagatgcgg acttacgtgg caaaggattc gataacgtgc
     tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
     agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac
     aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     ttgccaacga accggatacc cgtccgcaag tgcacgggaa tatttcgcca ctggcggaag
     caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg
     ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat
     ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg
     cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag
     ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg
     atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt
     tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct
     tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca
     tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac tctcctggcg
     caccatcgtc ggctacagcc tcgggaattg ctaccgagct cgaatttccc cgatcgttca
     aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     gatcgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct cctttcgctt
     tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca tcaaacagga
     ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccccagtaca
     ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     atatcctgcc a
//