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ID   PBI1013    preliminary; DNA; SYN; 5352 BP.
XX
AC   U12640; IG1036; X04996; X04995; V00087; J01541; M61152; M38354;
AC   S54410; M14641;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBI101.3 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RC   pBI121 from pBI101.1 & CMV 35S promoter
RC   pBI131 from pBI101.1 & tobacco rbcS promoter
RA   Jefferson R.A., Kavanaugh T.A., Bevan M.W.;
RT   "GUS fusions: beta-glucuronidase as a sensitive and versatile gene
RT   fusion marker in higher plants";
RL   EMBO J. 6:3901-3907(1987).
XX
RN   [2]
RP   1-5352
RC   pBI101.1, pBI101.2, pBI101.3 from pBIN19 & pRAJ260, GUS gene
RA   Wei W., Lindsey K.;
RT   "T-DNA sequence of the GUS gene fusion vector pBI101.3";
RL   Unpublished (1994).
XX
RN   [3]
RP   1-5352
RC   pBI101.3
RA   Wei W.;
RT   ;
RL   Submitted (22-JUL-1994) by:
RL   Wei W., Botany, University of Leicester, University Road,
RL   Leicester, LE1 7RH, UK.
XX
CC   NM (pBI101.3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(plant)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pBIN19)
CC   BR (pBI101.2)(pBI101.1)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBIN19 EcoRI 11777bp 6773..6773, MCS
FT                   2. pRAJ260 EcoRI-EcoRI, GUS gene
FT                   -> pBI101.3 5352bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO Agrobacterium Ti plasmid nopaline synthase"
FT   CDS             386..1180
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII); neomycin resistance gene (neo)
FT                   E. coli kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   terminator      0..0
FT                   /note="TER Agrobacterium Ti plasmid nopaline synthase"
FT   misc_binding    2498..2538
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             2554..4362
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT SnoBI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   3'UTR           4433..4685
FT                   /note="TER 3'UTR of the nopaline synthase gene"
FT   repeat_unit     5328..5352
FT                   /note="the left border repeat"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 5352 BP; 1274 A; 1346 C; 1420 G; 1312 T; 0 other;
     gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
     cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
     ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
     ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
     tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
     tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
     tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
     ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
     tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
     aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
     taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
     tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
     ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
     tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
     accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
     ctctagagga tccccgggta cggtcagtcc cttatgttac gtcctgtaga aaccccaacc
     cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga
     attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc
     agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat
     cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat
     gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc
     ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt
     atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt
     accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga
     atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg
     gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat
     ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc
     actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc
     tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc
     ggcatccggt cagtggcagt gaagggccaa cagttcctga ttaaccacaa accgttctac
     tttactggct ttggtcgtca tgaagatgcg gacttacgtg gcaaaggatt cgataacgtg
     ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg
     cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt
     gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac
     aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta
     caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt
     attgccaacg aaccggatac ccgtccgcaa gtgcacggga atatttcgcc actggcggaa
     gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
     gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga
     tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
     gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
     gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
     gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
     ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc
     ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
     atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc
     gcaccatcgt cggctacagc ctcgggaatt gctaccgagc tcgaatttcc ccgatcgttc
     aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     agatcgggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct
     ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg
     attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
     attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     tatatcctgc ca
//