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ID   PBIN19     preliminary; circular DNA; SYN; 11777 BP.
XX
AC   U09365; U12540; IG1039; X04996; X04995; V00087; J01541; M61152;
AC   M38354; S54410; M14641; ATCC37327;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Higher plants/E.coli plasmid vector pBin19 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from linker & pBR325, kan gene
RC   pUC9-R1 from pUC9
RC   pUC9-nopneodelta from pUC9-R1 & plasmid
RC   [pAT21-1 from pAT series]
RC   plasmid2 from pAT21-1 & pRK252
RC   pBIN6 from plasmid2 & pTiT37
RC   pBIN19 from pBIN6 & pTiT37 & M13mp19
RA   Bevan M.W.;
RT   "Binary Agrobacterium vectors for plant transformation";
RL   Nucleic Acids Res. 12:8711-8721(1984).
XX
RN   [2]
RC   pOTY8 from pJDB207 & pTiAch5
RC   pAL1050 from pOTY8 & R772, IS70
RC   pAL4404 from pTiT37
RA   Hoekema A., Hirsch P.R., Hooykaas P.J., Schilperoort R.A.;
RT   "A binary plant vector strategy based on separation of vir- and
RT   T-region of the Agrobacterium tumefaciens Ti-plasmid";
RL   Nature 303:179-180(1983).
XX
RN   [3]
RP   1-3213
RC   pBIN19 from pBIN6 & pTiT37 & M13mp19
RA   Wei W., Dekker B., Lindsey K.;
RT   "T-DNA sequence of plant transformation vector pBin19";
RL   Unpublished (1994).
XX
RN   [4]
RP   1-3213
RC   pBIN19
RA   Wei W.;
RT   ;
RL   Submitted (22-JUL-1994) by:
RL   Wei W., Botany, University of Leicester, University Road,
RL   Leicester, LE1 7RH, UK.
XX
RN   [5]
RP   1-11777
RC   pBIN19 from pBIN6 & pTiT37 & M13mp19
RA   Frisch D.A., Hardin S.H., Harris-Haller L., Yokubaitis N.T.,
RA   Thomas T.L., Hall T.C.;
RT   "Fortuitous ori and other sequences in Bin 19";
RL   Unpublished (1994).
XX
RN   [6]
RP   1-11777
RC   pBIN19
RA   Frisch D.A.;
RT   ;
RL   Submitted (04-MAY-1994) by:
RL   Frisch D.A., Biology, Texas A&M University, College Station,
RL   TX 77843-3155, USA.
XX
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--10.5. (ATCC staff)
CC   A vector for efficient transformation of higher plants in a binary
CC   plasmid system with pAL440. (personal communication)
CC   Medium is 1065 LB medium.
CC   NCBI gi: 520486
CC   NCBI gi: 529322
CC   NM (pBin19)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(ATCC)
CC   HO (Agrobacterium tumefaciens)(E.coli HB101)(higher plants)(E.coli)
CC   CP ()
CC   FN (cloning)(reporter)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pBI101)(pBI101.2)(pBI101.3)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR325 BamHI 5994bp 376..376
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene
FT                   -> plasmid 7535bp
FT                   1. plasmid Sau3A/MboI-Sau3A/MboI 330bp 1521..1851,
FT                   \ 5' kan/neo gene
FT                   Klenow:Klenow
FT                   BglII linker 6bp agatct:BglII linker 6bp agatct
FT                   BglII-PstI 216bp 1516..1732, 5' kan/neo gene
FT                   2. plasmid PstI-BamHI 402bp 2655..3057, 3' kan/neo
FT                   -> plasmid2 1150bp
FT                   1. pUC9 remove HindIII-EcoRI 30bp 234..264, MCS/2635bp
FT                   2. pTiT37 HindIII-EcoRI, nos promoter/terminator
FT                   -> plasmid3
FT                   1. plasmid2 BglII-BamHI 1150bp, kan/neo gene
FT                   2. plasmid3 BamHI, between cap and polyA
FT                   -> plasmid4
FT                   1. plasmid4 BamHI, between kan and polyA
FT                   fill in
FT                   -> plasmid5
FT                   1. plasmid5 EcoRI-HindIII, nos prom/kan gene/nos polyA
FT                   2. pTiT37 HindIII-HindIII 3200bp, nos gene/right T-DNA
FT                   -> plasmid6
FT                   1. pTiT7 KpnI-KpnI 3800bp, left T-DNA border
FT                   Klenow:Klenow
FT                   2. plasmid6 EcoRI-HindIII, nos prom/kan/polyA/nos/T
FT                   Klenow:
FT                   -> plasmid7 [T/nos prom/kan/polyA/nos/T]
FT                   1. plasmid7 remove SstII-SstII, nos gene
FT                   BstEII-BglII 5500bp, nos prom/kan/polyA/T
FT                   BAL31 nuclease remove 800bp from both ends
FT                   -> plasmid8 3900bp [right and left T-DNA borders]
FT                   1. RK2
FT                   -> pRK252
FT                   1. pBR322 4361bp
FT                   2. S. faecalis, aph III
FT                   -> pAT21-1
FT                   1. pAT21-1 HindIII-ClaI, kan gene
FT                   mung bean nuclease
FT                   2. pRK252 remove 4000bp, not tet/ori/kan
FT                   EcoRI, 3' to tet
FT                   mung bean nuclease
FT                   -> plasmid9
FT                   1. plasmid9 SmaI-SalI, tet gene
FT                   Klenow:Klenow
FT                   2. plasmid7 BglII-BstEII, T/nos prom/kan/polyA/nos/T
FT                   Klenow:Klenow
FT                   -> pBIN6 15000bp [no tet]
FT                   1. plasmid9 SmaI-SalI, tet gene
FT                   2. plasmid8 BglII-BstEII, T/nos prom/kan/polyA//T
FT                   BAL31 nuclease:BAL31 nuclease
FT                   -> plasmid10
FT                   1. plasmid10 EcoRI-EcoRI 1600bp, T-DNA
FT                   Klenow:Klenow
FT                   2. M13mp19 HaeII-HaeII 445bp 6006..6451,lacZalpha/MCS
FT                   Klenow:Klenow
FT                   3. pBIN6
FT                   -> pBIN19 11777bp"
FT   rep_origin      1..618
FT                   /note="ORI oriV"
FT   misc_feature    693..964
FT                   /note="GEN kilA gene"
FT   misc_feature    965..1315
FT                   /note="GEN NPTIII gene"
FT   misc_feature    1316..2085
FT                   /note="transposable element IS1"
FT   misc_feature    2086..3078
FT                   /note="GEN NPTIII gene"
FT   misc_feature    3079..4560
FT                   /note="GEN trfA"
FT   misc_feature    4561..5603
FT                   /note="GEN tetA"
FT   misc_feature    6043..6190
FT                   /note="T-DNA left border"
FT   misc_feature    6191..6321
FT                   /note="GEN lacI"
FT   rep_origin      6322..6622
FT                   /note="ORI M13 ori"
FT   misc_feature    6623..6917
FT                   /note="GEN lacZ"
FT   misc_feature    6918..7058
FT                   /note="GEN lacI"
FT   misc_feature    7059..7500
FT                   /note="GEN geneIII"
FT   terminator      7501..7756
FT                   /note="TER Nos terminator"
FT   misc_feature    7757..7968
FT                   /note="GEN ocd"
FT   misc_feature    7969..8952
FT                   /note="GEN NPTII gene"
FT   promoter        8953..9259
FT                   /note="PRO Nos promoter"
FT   misc_feature    9260..9421
FT                   /note="T-DNA right border"
FT   misc_feature    9434..10617
FT                   /note="GEN tetA"
FT   rep_origin      10610..10988
FT                   /note="ORI E.coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    10982..11765
FT                   /note="GEN traF"
XX
SQ   Sequence 11777 BP; 2735 A; 3158 C; 3383 G; 2501 T; 0 other;
     ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
     aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
     aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
     tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
     atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg
     ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg
     ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg
     gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg
     ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta
     atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc
     agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa
     tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
     tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
     ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc
     aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca
     aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
     tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
     ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
     cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
     gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
     gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
     ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
     aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
     gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
     gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
     tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
     agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
     tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
     ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
     tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
     acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
     tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
     acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
     accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
     gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
     gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
     ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
     gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
     cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
     tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg
     ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc
     gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat
     tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat
     ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga
     gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta
     catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc
     tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc
     cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat
     tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt
     ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg
     cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg
     attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac
     accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat
     ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac
     cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc
     gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg
     agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt
     ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc
     cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt
     tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta
     tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag
     cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca
     caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag
     ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg
     gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     cggccaacgc gcggggagag gcggtttgcg tattgggcca aagacaaaag ggcgacattc
     aaccgattga gggagggaag gtaaatattg acggaaatta ttcattaaag gtgaattatc
     accgtcaccg acttgagcca tttgggaatt agagccagca aaatcaccag tagcaccatt
     accattagca aggccggaaa cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt
     agcgacagaa tcaagtttgc ctttagcgtc agactgtagc gcgttttcat cggcattttc
     ggtcatagcc cccttattag cgtttgccat cttttcataa tcaaaatcac cggaaccaga
     gccaccaccg gaaccgcctc cctcagagcc gccaccctca gaaccgccac cctcagagcc
     accaccctca gagccgccac cagaaccacc accagagccg ccgccagcat tgacaggagg
     cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
     ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
     tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
     attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
     aaatgtttga acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg
     aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt
     tgatgtggac gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg
     ccgttgctgt cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg
     cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc
     gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     gcgaaacgat ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat
     tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
     gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga
     tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc
     agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc
     agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg
     atcaggggta tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga
     acgcgcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag
     tgtcaagcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga
     acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc
     agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
     ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
     ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
     gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
     gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
     gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
     gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
     tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
     tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gacgattgat
     caggaccgct gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc
     tccccctttc caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg
     ccctagcgtc caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc
     caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga
     ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc
     agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca
     atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca
     cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg
     acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt
     agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc
     aagaagagcg acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgag
     cgcctttgcg acgctca
//