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ID   PBLCAT2    preliminary; circular DNA; SYN; 4496 BP.
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AC   X64410; ATCC37527;
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DT   17-JUN-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBLCAT2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4496
RC   pBLCAT3 from pUC18 & cat gene & SV40 small t intron/polyA & linker
RC   pBLCAT2 from pBLCAT3 & HSV tk gene promoter
RA   Luckow B.H., Schutz G.;
RT   "CAT constructions with multiple unique restriction sites for
RT   functional analysis of eukaryotic promoters and regulatory elements";
RL   Nucleic Acids Res. 15:5490-5490(1987).
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CC   CAT vector for transient transfection into eukaryotic cells.
CC   Contains tk promoter for analysis of cis-acting elements in front
CC   of a heterologous promoter.
CC
CC   Developed to simplify construction of hybrid CAT genes.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.2; HindIII--4.2; BglII--4.2; ClaI--uncut. (ATCC staff)
CC   The ClaI site appears to have been destroyed during cloning.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBLCAT2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli DH1)(E.coli DH5)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (herpes simplex virus)(E.coli)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBLCAT3 remove BamHI-BglII 6bp 430..436,
FT                   \ MCS/4300bp
FT                   2. HSV BamHI-BglII 156bp, tk gene promoter -105 to +51
FT                   \ #X14112 46570..46720
FT                   -> pBLCAT2 4496bp"
FT   misc_binding    0..0
FT                   /note="MCS unique HindIII-SphI-(PstI)-SalI-XbaI-BamHI-
FT                   BglII-XhoI-SmaI-KpnI-SstI-(EcoRI)"
FT   promoter        429..598
FT                   /note="PRO herpes simplex virus thymidine kinase
FT                   gene (tk)"
FT   CDS             640..1299
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    1389..2002
FT                   /note="SV40 small T intron"
FT   polyA_signal    1999..2239
FT                   /note="PLA SV40 early gene"
FT   CDS             complement(3436..4296)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 4496 BP; 1188 A; 1045 C; 1070 G; 1193 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccggcccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
     cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg
     aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatctcg
     aggagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     attcgccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     atcttatcat gtctggatcg atccccgggt accgagctcg aattcgtaat catggtcata
     gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//