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ID   PBLCAT3    preliminary; circular DNA; SYN; 4344 BP.
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AC   X64409; ATCC37528;
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DT   17-JUN-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBLCAT3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4344
RC   pBLCAT3 from pUC18 & cat gene & SV40 small t intron/polyA & linker
RC   pBLCAT2 from pBLCAT3 & HSV tk gene promoter
RA   Luckow B.H., Schutz G.;
RT   "CAT constructions with multiple unique restriction sites for
RT   functional analysis of eukaryotic promoters and regulatory elements";
RL   Nucleic Acids Res. 15:5490-5490(1987).
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CC   Promoterless CAT vector for transient transfection experiments with
CC   eukaryotic cells. Allows the analysis of foreign promoters and
CC   enhancers.
CC
CC   Developed to simplify construction of hybrid CAT genes.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--4.4; HindIII--4.4; BglII--4.4; ClaI--uncut. (ATCC staff)
CC   The ClaI site appears to have been destroyed during cloning.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBLCAT3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli DH1)(E.coli DH5)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (E.coli)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 BamHI 2686bp 430..430, ori/amp gene
FT                   2. E. coli BamHI-BamHI 1400bp, cat gene/no promoter
FT                   3. SV40 BamHI-MboI 237bp 2534..2771,
FT                   \ small t intron/polyA
FT                   -> plasmid 4300bp
FT                   1. plasmid BamHI 4300bp, 3' to cat
FT                   BglII-XhoI linker 12bp agatctctcgag
FT                   BamHI 4300bp, 5' to SV40/pUC18 430..430
FT                   fill in, to make ClaI
FT                   -> pBLCAT3 4344bp"
FT   misc_binding    0..0
FT                   /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT                   BglII-XhoI-SmaI-KpnI-SstI-(EcoRI)"
FT   misc_feature    1237..1850
FT                   /note="SV40 small T intron splice site"
FT   polyA_signal    1847..2087
FT                   /note="PLA SV40 early"
FT   CDS             488..1147
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   promoterless"
FT   CDS             complement(3284..4144)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 4344 BP; 1159 A; 993 C; 1023 G; 1169 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccagatct ggatctcgag gagcttggcg agattttcag gagctaagga
     agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
     cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata
     agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
     ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat ccccgggtac
     cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     ggcgtatcac gaggcccttt cgtc
//