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ID   PBLCAT3F1  preliminary; circular DNA; SYN; 4865 BP.
XX
AC   IG9828;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli phagemid vector pBLCAT3.f1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBLCAT3.f1 from pBLCAT3 & pUC-f1
RC   pUC.A.1.5 from pUC18
RC   pJFCAT1 from pBLCAT3.f1 & pUC.A.1.5
RC   pGEM2-human-beta-globin from pGEM-2 & human beta-globin gene
RC   pbetaGloXH from pGEM-7 series & pGEM2-human-beta-globin
RC   pbetaGlo4 from pbetaGloXH & mouse tk promoter
RC   pbetaGlo4pA from pbetaGlo4 & pJFCAT1
RC   pTAG-1 from pbetaGlo4pA & pBLCAT3
RA   Fridovich-Keil J.L., Gudas J.M., Bryan I.B., Pardee A.B.;
RT   "Improved expression vectors for eukaryotic promoter/enhancer
RT   studies";
RL   Biotechniques 11:572-579(1991).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pBLCAT3.f1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)(E.coli HB101)(vertebrate cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBLCAT3)(pUC-f1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBLCAT3 remove SmaI-KpnI 6bp 2095..2101,
FT                   \ 4338bp
FT                   2. pUC-f1 KpnI-BamHI 519bp 413..932, phage f1 ori
FT                   :Klenow
FT                   -> pBLCAT3.f1 4857bp"
FT   -               1..2094
FT                   /note="pBLCAT3 1..2094 2094bp
FT                   SmaI = CCC^GGG
FT                   BamHI =  G^GATCC"
FT   -               2095..2621
FT                   /note="pUC-f1 409..935 527bp complement
FT                   KpnI = GGTAC^C"
FT   -               2622..4865
FT                   /note="pBLCAT3 2101..4344 2244bp"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 major late gene trimer"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-HindIII-SphI-PstI-SalI-BamHI-BglII-
FT                   XhoI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI-PstI-SalI-BglII-XhoI"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml),
FT                   from pBLCAT3"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene, from pBLCAT3"
FT   misc_feature    0..0
FT                   /note="SV40 small T intron splice site, from pBLCAT3"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI"
FT   misc_feature    0..0
FT                   /note="from pBLCAT3 KpnI-SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SstI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), from pBLCAT3"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   from pBLCAT3"
XX
SQ   Sequence 4865 BP; 1269 A; 1128 C; 1135 G; 1333 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccagatct ggatctcgag gagcttggcg agattttcag gagctaagga
     agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg
     cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata
     agtgataata agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt
     ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat ccccgatccc
     cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc
     ctgtttttgg ggcttttctg attatcaacc ggggtgggta ccgagctcga attcgtaatc
     atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     tcgtc
//