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ID   PBLCAT5    preliminary; circular DNA; SYN; 4404 BP.
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AC   M80483; ATCC77412;
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DT   25-FEB-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pBLCAT5 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4404
RC   pBLCAT5 from pBLCAT2 & SV40
RC   pBLCAT6 from pBLCAT3 & SV40
RA   Boshart M., Klueppel M., Schmidt A., Schuetz G., Luckow B.H.;
RT   "CAT reporter constructs with low background activity";
RL   Gene 110:129-130(1992).
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CC   Reporter plasmid for analysis of enhancer sequences in vertebrate
CC   cells, using the herpes simplex virus (HSV) thymidine kinase promoter
CC   to drive expression of the cat reporter gene.
CC   Constructed from pBLCAT2 (ATCC 37527) by deletion of a 467 bp fragment
CC   immediately upstream of the 5' polylinker and replacement by two
CC   fragments, each containing the polyadenylation signal of the SV40
CC   large T gene.
CC   The vector contains the following restriction sites (approximate kb
CC   from nt 1):  BamHI--0.03;
CC   BglI--3.23; ClaI--1.84; EcoRI--0.06, 0.46, 1.86; HindIII--0.0;
CC   NcoI--0.76; PstI--0.01, 0.16; PvuI--3.48; PvuII--0.35, 2.04;
CC   SacI--1.86; XhoI--0.20.
CC   The order of the major features in the plasmid is:  two SV40
CC   polyadenylation signals - HindIII/MCS/BamHI - HSV tk promoter - cat -
CC   SV40 small T splice signal - SV40 polyadenylation signal -
CC   ClaI/MCS/SstI - pMB1 ori - ampR. [1]
CC   Deposited by: M.D.Boshart
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   NM (pBLCAT5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBLCAT2 remove AatII-HindIII 466bp
FT                   \ 4428..4496..398/4030bp
FT                   2. SV40 BamHI-HpaI 135bp 2534..2669, complement
FT                   3. SV40 BamHI-BclI 237bp 2534..2771, complement
FT                   -> pBLCAT5 4402bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-SalI-XbaI-BamHI"
FT   misc_binding    0..0
FT                   /note="MCS ClaI-SmaI-KpnI-SstI"
FT   promoter        31..200
FT                   /note="PRO HSV thymidine kinase gene"
FT   CDS             242..901
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    991..1604
FT                   /note="SPL SV40 small T splice site"
FT   polyA_signal    1601..1841
FT                   /note="PLA SV40 polyadenylation signal"
FT   CDS             complement(3038..3898)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), partial"
FT   polyA_signal    4030..4164
FT                   /note="PLA SV40 polyadenylation signal"
FT   polyA_signal    4161..4401
FT                   /note="PLA SV40 polyadenylation signal"
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SQ   Sequence 4404 BP; 1196 A; 1005 C; 976 G; 1227 T; 0 other;
     aagcttgcat gcctgcaggt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt
     ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
     caacagcgtg ccgcagatct cgaggagctt ggcgagattt tcaggagcta aggaagctaa
     aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
     tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
     aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
     tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct
     cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt
     gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     gtatcttatc atgtctggat ctcg
//