back Return to this vector's summary.
ID   PBLCAT6    preliminary; circular DNA; SYN; 4256 BP.
XX
AC   M80484; ATCC77413;
XX
DT   25-FEB-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pBLCAT6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4256
RC   pBLCAT5 from pBLCAT2 & SV40
RC   pBLCAT6 from pBLCAT3 & SV40
RC   [pBLCAT8+ from pBLCAT series]
RA   Boshart M., Klueppel M., Schmidt A., Schuetz G., Luckow B.H.;
RT   "CAT reporter constructs with low background activity";
RL   Gene 110:129-130(1992).
XX
CC   Promoter-cloning vector for analysis of promoters in vertebrate
CC   cells, using the CAT reporter gene.
CC   Constructed from pBLCAT3 (ATCC37528) by deletion of a 467 bp fragment
CC   immediately upstream from the 5' polylinker and replacement by two
CC   fragments, each containing the
CC   polyadenylation signal of the SV40 large T gene.
CC   The vector contains the following restriction sites (approximate kb
CC   from nt 1):  BglI--3.07;
CC   ClaI--1.69; EcoRI--0.30, 1.71; HindIII--0.0; NcoI--0.60; PvuI--3.33;
CC   PvuII--0.20, 1.89; SacI--1.70; XhoI--0.05.
CC   The order of the major features in the plasmid is:
CC   two SV40 polyadenylation signals - HindIII/MCS/XhoI - cat -
CC   SV40 small T splice site - SV40 polyadenylation
CC   signal - ClaI/MCS/SstI - pMB1 ori - ampR. [1]
CC   Deposited by: M.D.Boshart
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   NM (pBLCAT6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBLCAT3 remove AatII-HindIII 464bp
FT                   \ 4280..4344..400, 3880bp
FT                   2. SV40 BamHI-HpaI 135bp 2534..2669, complement
FT                   3. SV40 BamHI-BclI 237bp 2534..2771, complement
FT                   -> pBLCAT6 4256bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-BglII-
FT                   XhoI"
FT   misc_binding    0..0
FT                   /note="MCS ClaI-SmaI-KpnI-SstI"
FT   CDS             90..749
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    839..1452
FT                   /note="SPL SV40 small T splice site"
FT   polyA_signal    1449..1689
FT                   /note="PLA SV40 polyadenylation signal"
FT   CDS             complement(2886..3746)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp), partial"
FT   polyA_signal    3878..4012
FT                   /note="PLA SV40 polyadenylation signal"
FT   polyA_signal    4009..4249
FT                   /note="PLA SV40 polyadenylation signal"
XX
SQ   Sequence 4256 BP; 1168 A; 954 C; 930 G; 1204 T; 0 other;
     aagcttgcat gcctgcaggt cgactctaga ggatccagat ctggatctcg aggagcttgg
     cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga
     tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     gtctggatcg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg taacttgttt attgcagctt
     ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcataa
     tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcg
//