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ID   PBLUBAC    preliminary; circular DNA; SYN; 13994 BP.
XX
AC   IG1040;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBac - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBac)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA ()
CC   BR ()
CC   OF (pBlueBacHisA)(pBlueBacHisB)(pBlueBacHisC)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. AcMNPV, polyhedrin/ETL promoters
FT                   3. E. coli, lacZ gene
FT                   -> pBlueBac 13994bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   misc_binding    6773..6773
FT                   /note="SIT SacI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 13994 BP; 3608 A; 3288 C; 3283 G; 3815 T; 0 other;
     aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
     gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
     ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
     gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
     ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
     gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
     gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
     ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
     cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
     aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
     tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
     tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
     gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
     ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
     cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
     ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
     tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     atcacaaact ggaaatgtct atcaatatat agttgctgat tcaggatcca gacatgataa
     gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gagtcgagat
     ccccctcgcc cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg
     cgctcagctg gaattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca
     tatggaaacc gtcgatattc agccatgtgc cttcttccgc gtgcagcaga tggcgatggc
     tggtttccat cagttgctgt tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc
     actggtgtgg gccataattc aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga
     agacgtacgg ggtatacatg tctgacaatg gcagatccca gcggtcaaaa caggcggcag
     taaggcggtc gggatagttt tcttgcggcc ctaatccgag ccagtttacc cgctctgcta
     cctgcgccag ctggcagttc aggccaatcc gcgccggatg cggtgtatcg ctcgccactt
     caacatcaac ggtaatcgcc atttgaccac taccatcaat ccggtaggtt ttccggctga
     taaataaggt tttcccctga tgctgccacg cgtgagcggt cgtaatcagc accgcatcag
     caagtgtatc tgccgtgcac tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct
     tccagcgttc gacccaggcg ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt
     tatccagcgg tgcacgggtg aactgatcgc gcagcggcgt cagcagttgt tttttatcgc
     caatccacat ctgtgaaaga aagcctgact ggcggttaaa ttgccaacgc ttattaccca
     gctcgatgca aaaatccatt tcgctggtgg tcagatgcgg gatggcgtgg gacgcggcgg
     ggagcgtcac actgaggttt tccgccagac gccactgctg ccaggcgctg atgtgcccgg
     cttctgacca tgcggtcgcg ttcggttgca ctacgcgtac tgtgagccag agttgcccgg
     cgctctccgg ctgcggtagt tcaggcagtt caatcaactg tttaccttgt ggagcgacat
     ccagaggcac ttcaccgctt gccagcggct taccatccag cgccaccatc cagtgcagga
     gctcgttatc gctatgacgg aacaggtatt cgctggtcac ttcgatggtt tgcccggata
     aacggaactg gaaaaactgc tgctggtgtt ttgcttccgt cagcgctgga tgcggcgtgc
     ggtcggcaaa gaccagaccg ttcatacaga actggcgatc gttcggcgta tcgccaaaat
     caccgccgta agccgaccac gggttgccgt tttcatcata tttaatcagc gactgatcca
     cccagtccca gacgaagccg ccctgtaaac ggggatactg acgaaacgcc tgccagtatt
     tagcgaaacc gccaagactg ttacccatcg cgtgggcgta ttcgcaaagg atcagcgggc
     gcgtctctcc aggtagcgaa agccattttt tgatggacca tttcggcaca gccgggaagg
     gctggtcttc atccacgcgc gcgtacatcg ggcaaataat atcggtggcc gtggtgtcgg
     ctccgccgcc ttcatactgc accgggcggg aaggatcgac agatttgatc cagcgataca
     gcgcgtcgtg attagcgccg tggcctgatt cattccccag cgaccagatg atcacactcg
     ggtgattacg atcgcgctgc accattcgcg ttacgcgttc gctcatcgcc ggtagccagc
     gcggatcatc ggtcagacga ttcattggca ccatgccgtg ggtttcaata ttggcttcat
     ccaccacata caggccgtag cggtcgcaca gcgtgtacca cagcggatgg ttcggataat
     gcgaacagcg cacggcgtta aagttgttct gcttcatcag caggatatcc tgcaccatcg
     tctgctcatc catgacctga ccatgcagag gatgatgctc gtgacggtta acgcctcgaa
     tcagcaacgg cttgccgttc agcagcagca gaccattttc aatccgcacc tcgcggaaac
     cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg
     cacgatagag attcgggatt tcggcgctcc acagtttcgg gttttcgacg ttcagacgta
     gtgtgacgcg atcggcataa ccaccacgct catcgataat ttcaccgccg aaaggcgcgg
     tgccgctggc gacctgcgtt tcaccctgcc ataaagaaac tgttacccgt aggtagtcac
     gcaactcgcc gcacatctga acttcagcct ccagtacagc gcggctgaaa tcatcattaa
     agcgagtggc aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt
     cacggaaaat gccgctcatc cgccacatat cctgatcttc cagataactg ccgtcactcc
     aacgcagcac catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa
     attcagacgg caaacgactg tcctggccgt aaccgaccca gcgcccgttg caccacagat
     gaaacgccga gttaacgcca tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt
     catcaacatt aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga acaaacggcg
     gattgaccgt aatgggatag gttacgttgg tgtagatggg cgcatcgtaa ccgtgcatct
     gccagtttga ggggacgacg acagtatcgg cctcaggaag atcgcactcc agccagcttt
     ccggcaccgc ttctggtacc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca
     actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     aaacgacggg atctatcatt tttaggatcc agtgattcta atctgcagct gctctttgat
     acaactaatt ttacgacgac gatgcgagct cttattcaac cgagcgtgca tgtttgcaat
     cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg ctggacacca
     cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa tgcaaacttt
     ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag cgagtgcctt
     cgactttttc gtgtccagtg tggcttgatt catggagata attaaaatga taaccatctc
     gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     attccggatt attcataccg tcccaccatc gggcgtgcta gcggatcctt tcctgggacc
     cggcaagaac caaaaactca ctctcttcaa ggaaatccgt aatgttaaac ccgacacgat
     gaagcttgtc gttggatgga aaggaaaaga gttctacagg gaaacttgga cccgcttcat
     ggaagacagc ttccccattg ttaacgacca agaagtgatg gatgttttcc ttgttgtcaa
     catgcgtccc actagaccca accgttgtta caaattcctg gcccaacacg ctctgcgttg
     cgaccccgac tatgtacctc atgacgtgat taggatcgtc gagccttcat gggtgggcag
     caacaacgag taccgcatca gcctggctaa gaagggcggc ggctgcccaa taatgaacct
     tcactctgag tacaccaact cgttcgaaca gttcttcgat cgtgtcatct gggagaactt
     ctacaagccc atcgtttaca tcggtaccga ctctgctgaa gaggaggaaa ttctccttga
     agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt tcactggtcc
     ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag attctgtgcg
     ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt
     agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa
     tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt
     ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa
     atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg
     ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta
     caattgactc gacgtaaaca cgttaaataa agcttggaca tatttaacat cgggcgtgtt
     agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga
     agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat
     caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt
     caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg
     tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc
     taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg
     tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac
     tattgtactg gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt
     ttcgtttcta atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg
     ttccgtcggc attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg
     aggcgctgga atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg
     ttctggttta gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac
     aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt
     ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc
     gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctccg
     cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct
     gtcgtcgacg tacatgtatg ctttgttgtc aaaaacgtcg ttggcaagct ttaaaatatt
     taaaagaaca tctctgttca gcaccactgt gttgtcgtaa atgttgtttt tgataatttg
     cgcttccgca gtatcgacac gttcaaaaaa ttgatgcgca tcaattttgt tgttcctatt
     attgaataaa taagattgta cagattcata tctacgattc gtcatggcca ccacaaatgc
     tacgctgcaa acgctggtac aattttacga aaactgcaaa aacgtcaaaa ctcggtataa
     aataatcaac gggcgctttg gcaaaatatc tattttatcg cacaagccca ctagcaaatt
     gtatttgcag aaaacaattt cggcgcacaa ttttaacgct gacgaaataa aagttcacca
     gttaatgagc gaccacccaa attttataaa aatctatttt aatcacggtt ccatcaacaa
     ccaagtgatc gtgatggact acattgactg tcccgattta tttgaaacac tacaaattaa
     aggcgagctt tcgtaccaac ttgttagcaa tattattaga cagctgtgtg aagcgctcaa
     cgatttgcac aagcacaatt tcatacacaa cgacataaaa ctcgaaaatg tcttatattt
     cgaagcactt gatcgcgtgt atgtttgcga ttacggattg tgcaaacacg aaaactcact
     tagcgtgcac gacggcacgt tggagtattt tagtccggaa aaaattcgac acacaactat
     gcacgtttcg tttgactggt acgcggcgtg ttaacataca agttgctaac cggcggccga
     cacccatttg aaaaaagcga agacgaaatg ttggacttga atagcatgaa gcgtcgtcag
     caatacaatg acattggcgt tttaaaacac gttcgtaacg ttaacgctcg tgactttgtg
     tactgcctaa caagatacaa catagattgt agactcacaa attacaaaca aattataaaa
     catgagtttt tgtcgtaaaa atgccacttg ttttacgagt agaattacgc gccctgtagc
     ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     aaaatattaa cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt
     ttctgattat caaccggggt aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     cgacggccag tgcc
//