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ID   PBLUBAC2   preliminary; circular DNA; SYN; 10271 BP.
XX
AC   IG1160;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBac2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBac2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA ()
CC   BR ()
CC   OF (pBlueBacHisA)(pBlueBacHisB)(pBlueBacHisC)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. AcMNPV, polyhedrin/ETL promoters
FT                   3. E. coli, lacZ gene
FT                   -> pBlueBac2 10271bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   misc_binding    6773..6773
FT                   /note="SIT SacI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 10271 BP; 2735 A; 2489 C; 2441 G; 2606 T; 0 other;
     tcgacagatc tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcctctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac
     ataaccttat gtatcataca catacgattt aggtgacact atagaactcg agtcgacgac
     agcgaagtgt ctcgccacta caatgctgta gtaaaaatga aaaggcttgt tatcggcgtg
     cggagcttga gacaatctct ttacaataca attgcttaca ttgagcggtt gttaaatatc
     ggcacggtaa acgatagcga aattacaatg cttatagcag atttttacga tttgtattcc
     aattataata ttgaattgcc accaccccaa gcgctgcctc gaagcagacg accttccgtt
     gtgcagccag cggcgcctgc gccggtgccc acaatcgtgc gcgaacaaac taaaccagaa
     caaattatac cggcggcacc gccgccacca ccttctcccg tgcctaacat tccagcgcct
     ccaccaccac caccaccatc gatgtctgaa ttgccgcccg ctccaccaat gccgacggaa
     cctcaacccg ctgcaccttt agacgacaga caacaattgt tggaagctat tagaaacgaa
     aaaaatcgca ctcgtctcag accggtcaaa ccaaaaacgg cgcccgaaac cagtacaata
     gttgaggtgc cgactgtgtt gcctaaagag acatttgagc ctaaaccgcc gtctgcatca
     ccgccaccac ctccgcctcc gcctccgccg ccagccccgc ctgcgcctcc accgatggta
     gatttatcat cagctccacc accgccgcca ttagtagatt tgccgtctga aatgttacca
     ccgcctgcac catcgctttc taacgtgttg tctgaattaa aatcgggcac agttagattg
     aaacccgccc aaaaacgccc gcaatcagaa ataattccaa aaagctcaac tacaaatttg
     atcgcggacg tgttagccga cacaattaat aggcgtcgtg tggctatggc aaaatcgtct
     tcggaagcaa cttctaacga cgagggttgg gacgacgacg ataatcggcc taataaagct
     aacacgcccg atgttaaata tgtccaagct ttatttaacg tgtttacgtc gagtcaattg
     tacactaacg acagtgatga aagaaataca aaagcgcata atattttgaa cgacgtcgaa
     cctttattac aaaacaaaac acaaacgaat atcgacaaag ctagattgct gctacaagat
     ttggcaagtt ttgtggcgtt gagcgaaaat ccattagata gtccagccat cggttcggaa
     aaacaaccct tgtttgaaac taatcgaaac ctattttaca aatctattga ggatttaata
     tttaaattca gatataaaga cgctgaaaat catttgattt tcgctctaac ataccaccct
     aaagattata aatttaatga attattaaaa tacgtacaac aattgtctgt aaatcaacaa
     cgcacagaat ctagcgctta ataaatgtac taataacaat gtatcgtgtt ttaatacgcc
     ggaccagtga acagaggtgc gtctggtgca aactccttta ctttgaacac cagggaaact
     tcaaggagaa tttcctcctc ttcagcagag tcggtaccga tgtaaacgat gggcttgtag
     aagttctccc agatgacacg atcgatgaac tgttcgaacg agttggtgta ctcagagtga
     aggttcatta ttgggcagcc gccgcccttc ttagccaggc tgatgcggta ctcgttgttg
     ctgcccaccc atgaaggctc gacgatccta atcacgtcat gaggtacata gtcggggtcg
     caacgcagag cgtgttgggc caggaatttg taacaacggt tgggtctagt gggacgcatg
     ttgacaacaa ggaaaacatc catcacttct tggtcgttaa caatggggaa gctgtcttcc
     atgaagcggg tccaagtttc cctgtagaac tcttttcctt tccatccaac gacaagcttc
     atcgtgtcgg gtttaacatt acggatttcc ttgaagagag tgagtttttg gttcttgccg
     ggtcccagga aaggatccgc tagcacgccc gatggtggga cggtatgaat aatccggaat
     atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc
     gagatggtta tcattttaat tatctccatg atatcaagcc acactggaca cgaaaaagtc
     gaaggcactc gcttcgatca atggacaaag aatcaatcgt tcgtaggcaa cagaatgtcg
     gaaagtttgc attggatgcg cggcgggtcc aacttgccgc aaaactgcgg cgagttcaac
     gtggtgtcca gcctgttgat gtgcaacaat acgataatga aaaattgata acgcttgcac
     gattgcaaac atgcacgctc ggttgaataa aagctcgcat cgtcgtcgta aaattagttg
     tatcaaagag cagctgcaat tagaatcact gctaaaaatg atagatcccg tcgttttaca
     acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
     gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
     cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
     tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
     ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
     tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
     agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
     gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
     tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
     tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
     caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
     cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
     gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
     ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
     tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
     tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
     gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
     cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
     gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
     cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
     gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
     gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
     cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
     ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
     ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
     agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
     cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
     gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
     cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
     tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
     gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
     tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggcg agggggatct
     cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     catgtctgga tctgatatca gcaactatat attgatagac atttccagtt tgtgatatta
     gtttgtgcgt ctcattacaa tggctgttat ttttaacaac aaacaactgc tcgcagacaa
     tagtatagaa aagggaggtg aactgttttt gtttaacggt tcgtacaaca ttttggaaag
     ttatgttaat ccggtgctgc taaaaaatgg tgtaattgaa ctagaagaag ctgcgtacta
     tgccggcaac atattgtaca aaaccgacga tcccaaattc attgattata taaatttaat
     aattaaagca acacactccg aagaactacc agaaaatagc actgttgtaa attacagaaa
     aactatgcgc agcggtacta tacaccccat taaaaaagac atatatattt atgacaacaa
     aaaatttact ctatacgata gatacatata tggatacgat aataactatg ttaattttta
     tgaggagaaa aatgaaaaag agaaggaata cgaagaagaa gacgacaagg cgtctagttt
     atgtgaaaat aaaattatat tgtcgcaaat taactgtgaa tcatttgaaa atgattttaa
     atattacctc agcgattata actacgcgtt ttcaattata gataatacta caaatgttct
     tgttgcgttt ggtttgtatc gttaataaaa aacaaattta gcatttataa ttgttttatt
     attcaataat tacaaatagg attgagaccc ttgcagttgc cagcaaacgg acagagcttg
     tcgacagttt tcattttttg gcagtttggt ggtttataca catcttggga tttgattaaa
     agatgcatga cttcacccac acacttggag tcgtggttgt agaaatgcgt cagagccttg
     attaaacatc ggttttcaca cttggcacac gaaaccacgt ttgcaatgta acagtttagt
     ataaacctct tcctgtacat accgccgcgc ggtacattgt tttctttcaa atcgttcaat
     atttttttta tacacggagg catattaatc ttgttgttca gcatgtcgac gacatttttc
     tttccttcta cgcattcttt catacaaatg tttctcttgc tgcgtttttt aaaattggaa
     tttttaattt cattaatagt gggcggagcc gttaacagca tgtacaatcg cagtccggaa
     attttgacca aaaccccgcc attgtagaat accgtgtatg gagttagggt caatttatca
     ataaaatcat cgggatcaat taaatatgta gctttcttgt ctaaacatga cgctctaatg
     agcggactcc acacgttata cgatgcattc gccatacttc gcggcttctc gcaccagcgg
     tcctgattcg actaacggtc gattggatgg cttaatagtt ttaacgtcac cgacatactc
     ggtaattttg tagtcggcag ttcttttggg cgtgatcgtt gtcactacgg gggtcatgtc
     atcgcttcgt aaaaacttat tacaatcaaa gctcgtgccg gaacgcgtgc acagatcgtg
     cacgttgcgt cgcaagctat ttaattcgcg ctttatttga tctctatcat agtcgtgttg
     agatgcgaca acgggcgcat tgttaattct tgacgtgcga tacataatat attattggat
     aaagttgcat taaatgaaac taacttacaa gatggctagt ttgttaaaat acgcgctgcg
     cttgactcgg gaatacaaag aaaacattat tccacacttt gatcacttga ctcgattgcg
     cgatttaatc gacggcatga ttaaaagcga ggatgtacaa cgttttaatc gcactaatcg
     caatgattta atttcggctt gcatgcaaat caacgttcgg acgtacatgc ccaacgccac
     gatagatatg cgcaaacaac ccaactgtat atattttcga atttgccaat attgccactt
     ggaggccgac gtgccttcgc ccgacgatca ttcggtgtac agatacttgt gcgtcgcgtg
     cggcacgccg ctggtcatcg accacccgct cgacgtgttc ggccacacgg aggaaggcgt
     caacgaactg c
//