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ID   PBLUBACIII preliminary; circular DNA; SYN; 10241 BP.
XX
AC   IG1041;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBacIII - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBacIII)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA ()
CC   BR ()
CC   OF (pBlueBacHisA)(pBlueBacHisB)(pBlueBacHisC)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. AcMNPV, polyhedrin/ETL promoters
FT                   3. E. coli, lacZ gene
FT                   -> pBlueBacIII 10241bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   misc_binding    6773..6773
FT                   /note="SIT SacI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 10241 BP; 2592 A; 2430 C; 2489 G; 2730 T; 0 other;
     gcagttcgtt gacgccttcc tccgtgtggc cgaacacgtc gagcgggtgg tcgatgacca
     gcggcgtgcc gcacgcgacg cacaagtatc tgtacaccga atgatcgtcg ggcgaaggca
     cgtcggcctc caagtggcaa tattggcaaa ttcgaaaata tatacagttg ggttgtttgc
     gcatatctat cgtggcgttg ggcatgtacg tccgaacgtt gatttgcatg caagccgaaa
     ttaaatcatt gcgattagtg cgattaaaac gttgtacatc ctcgctttta atcatgccgt
     cgattaaatc gcgcaatcga gtcaagtgat caaagtgtgg aataatgttt tctttgtatt
     cccgagtcaa gcgcagcgcg tattttaaca aactagccat cttgtaagtt agtttcattt
     aatgcaactt tatccaataa tatattatgt atcgcacgtc aagaattaac aatgcgcccg
     ttgtcgcatc tcaacacgac tatgatagag atcaaataaa gcgcgaatta aatagcttgc
     gacgcaacgt gcacgatctg tgcacgcgtt ccggcacgag ctttgattgt aataagtttt
     tacgaagcga tgacatgacc cccgtagtga caacgatcac gcccaaaaga actgccgact
     acaaaattac cgagtatgtc ggtgacgtta aaactattaa gccatccaat cgaccgttag
     tcgaatcagg accgctggtg cgagaagccg cgaagtatgg cgaatgcatc gtataacgtg
     tggagtccgc tcattagagc gtcatgttta gacaagaaag ctacatattt aattgatccc
     gatgatttta ttgataaatt gaccctaact ccatacacgg tattctacaa tggcggggtt
     ttggtcaaaa tttccggact gcgattgtac atgctgttaa cggctccgcc cactattaat
     gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcag
     atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     ctctagagtc gagatccccc tcgcccggtt attattattt ttgacaccag accaactggt
     aatggtagcg accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt
     cgccaccaat ccccatatgg aaaccgtcga tattcagcca tgtgccttct tccgcgtgca
     gcagatggcg atggctggtt tccatcagtt gctgttgact gtagcggctg atgttgaact
     ggaagtcgcc gcgccactgg tgtgggccat aattcaattc gcgcgtcccg cagcgcagac
     cgttttcgct cgggaagacg tacggggtat acatgtctga caatggcaga tcccagcggt
     caaaacaggc ggcagtaagg cggtcgggat agttttcttg cggccctaat ccgagccagt
     ttacccgctc tgctacctgc gccagctggc agttcaggcc aatccgcgcc ggatgcggtg
     tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg accactacca tcaatccggt
     aggttttccg gctgataaat aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa
     tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa caacgctgct tcggcctggt
     aatggcccgc cgccttccag cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac
     ttacgccaat gtcgttatcc agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca
     gttgtttttt atcgccaatc cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc
     aacgcttatt acccagctcg atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg
     cgtgggacgc ggcggggagc gtcacactga ggttttccgc cagacgccac tgctgccagg
     cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga
     gccagagttg cccggcgctc tccggctgcg gtagttcagg cagttcaatc aactgtttac
     cttgtggagc gacatccaga ggcacttcac cgcttgccag cggcttacca tccagcgcca
     ccatccagtg caggagctcg ttatcgctat gacggaacag gtattcgctg gtcacttcga
     tggtttgccc ggataaacgg aactggaaaa actgctgctg gtgttttgct tccgtcagcg
     ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat acagaactgg cgatcgttcg
     gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt gccgttttca tcatatttaa
     tcagcgactg atccacccag tcccagacga agccgccctg taaacgggga tactgacgaa
     acgcctgcca gtatttagcg aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc
     aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca ttttttgatg gaccatttcg
     gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg
     tggccgtggt gtcggctccg ccgccttcat actgcaccgg gcgggaagga tcgacagatt
     tgatccagcg atacagcgcg tcgtgattag cgccgtggcc tgattcattc cccagcgacc
     agatgatcac actcgggtga ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca
     tcgccggtag ccagcgcgga tcatcggtca gacgattcat tggcaccatg ccgtgggttt
     caatattggc ttcatccacc acatacaggc cgtagcggtc gcacagcgtg taccacagcg
     gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt gttctgcttc atcagcagga
     tatcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac
     ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc
     gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt
     gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt
     cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac
     cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta
     cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc
     tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat
     gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat
     aactgccgtc actccaacgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa
     atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc
     cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt
     cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg
     tgggaacaaa cggcggattg accgtaatgg gataggttac gttggtgtag atgggcgcat
     cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc
     actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc
     cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct
     gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca
     tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg
     ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa
     tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag
     cgagtgcctt cgactttttc gtgtccagtg tggcttgata tcatggagat aattaaaatg
     ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     aacctataaa tattccggat tattcatacc gtcccaccat cgggcgtgct agcggatccg
     agctcgagat ctgcagctgg taccatggaa ttcgaagctt gtcgttggat ggaaaggaaa
     agagttctac agggaaactt ggacccgctt catggaagac agcttcccca ttgttaacga
     ccaagaagtg atggatgttt tccttgttgt caacatgcgt cccactagac ccaaccgttg
     ttacaaattc ctggcccaac acgctctgcg ttgcgacccc gactatgtac ctcatgacgt
     gattaggatc gtcgagcctt catgggtggg cagcaacaac gagtaccgca tcagcctggc
     taagaagggc ggcggctgcc caataatgaa ccttcactct gagtacacca actcgttcga
     acagttcatc gatcgtgtca tctgggagaa cttctacaag cccatcgttt acatcggtac
     cgactctgct gaagaggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga
     gtttgcacca gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta
     ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac
     gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa
     atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa
     taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa
     tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc
     gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc
     ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa
     taaagctagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta
     attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat
     ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg
     gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct
     caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg
     tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca
     attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg
     gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg
     gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca
     cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag
     gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg
     ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt
     aagcaattgt attgtaaaga gattgtctca agctccgcac gccgataaca agccttttca
     tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgactcga gttctatagt
     gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     acgacaatat gtccatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     cgtcatcacc gaaacgcgcg agaggaaagg gcctcgtgat acgcctattt ttataggtta
     atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     cgcctctccc cgcgcgttgg ccgattcatt aatgcaggtt aacctggctt atcgaaatta
     atacgactca ctatagggag accggcagat cgatctgtcg a
//