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ID   PBLUBCHISA preliminary; circular DNA; SYN; 10304 BP.
XX
AC   IG1042;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBacHisA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBacHisA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA (pBlueBacIII)
CC   BR (pBlueBacHisB)(pBlueBacHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBlueBac
FT                   2. oligo, ATG/histidine repeats/enterokinase site
FT                   -> pBlueBacHisA 10304bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   misc_feature    0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 10304 BP; 2611 A; 2436 C; 2512 G; 2745 T; 0 other;
     gcagttcgtt gacgccttcc tccgtgtggc cgaacacgtc gagcgggtgg tcgatgacca
     gcggcgtgcc gcacgcgacg cacaagtatc tgtacaccga atgatcgtcg ggcgaaggca
     cgtcggcctc caagtggcaa tattggcaaa ttcgaaaata tatacagttg ggttgtttgc
     gcatatctat cgtggcgttg ggcatgtacg tccgaacgtt gatttgcatg caagccgaaa
     ttaaatcatt gcgattagtg cgattaaaac gttgtacatc ctcgctttta atcatgccgt
     cgattaaatc gcgcaatcga gtcaagtgat caaagtgtgg aataatgttt tctttgtatt
     cccgagtcaa gcgcagcgcg tattttaaca aactagccat cttgtaagtt agtttcattt
     aatgcaactt tatccaataa tatattatgt atcgcacgtc aagaattaac aatgcgcccg
     ttgtcgcatc tcaacacgac tatgatagag atcaaataaa gcgcgaatta aatagcttgc
     gacgcaacgt gcacgatctg tgcacgcgtt ccggcacgag ctttgattgt aataagtttt
     tacgaagcga tgacatgacc cccgtagtga caacgatcac gcccaaaaga actgccgact
     acaaaattac cgagtatgtc ggtgacgtta aaactattaa gccatccaat cgaccgttag
     tcgaatcagg accgctggtg cgagaagccg cgaagtatgg cgaatgcatc gtataacgtg
     tggagtccgc tcattagagc gtcatgttta gacaagaaag ctacatattt aattgatccc
     gatgatttta ttgataaatt gaccctaact ccatacacgg tattctacaa tggcggggtt
     ttggtcaaaa tttccggact gcgattgtac atgctgttaa cggctccgcc cactattaat
     gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcag
     atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     ctctagagtc gagatccccc tcgcccggtt attattattt ttgacaccag accaactggt
     aatggtagcg accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt
     cgccaccaat ccccatatgg aaaccgtcga tattcagcca tgtgccttct tccgcgtgca
     gcagatggcg atggctggtt tccatcagtt gctgttgact gtagcggctg atgttgaact
     ggaagtcgcc gcgccactgg tgtgggccat aattcaattc gcgcgtcccg cagcgcagac
     cgttttcgct cgggaagacg tacggggtat acatgtctga caatggcaga tcccagcggt
     caaaacaggc ggcagtaagg cggtcgggat agttttcttg cggccctaat ccgagccagt
     ttacccgctc tgctacctgc gccagctggc agttcaggcc aatccgcgcc ggatgcggtg
     tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg accactacca tcaatccggt
     aggttttccg gctgataaat aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa
     tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa caacgctgct tcggcctggt
     aatggcccgc cgccttccag cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac
     ttacgccaat gtcgttatcc agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca
     gttgtttttt atcgccaatc cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc
     aacgcttatt acccagctcg atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg
     cgtgggacgc ggcggggagc gtcacactga ggttttccgc cagacgccac tgctgccagg
     cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga
     gccagagttg cccggcgctc tccggctgcg gtagttcagg cagttcaatc aactgtttac
     cttgtggagc gacatccaga ggcacttcac cgcttgccag cggcttacca tccagcgcca
     ccatccagtg caggagctcg ttatcgctat gacggaacag gtattcgctg gtcacttcga
     tggtttgccc ggataaacgg aactggaaaa actgctgctg gtgttttgct tccgtcagcg
     ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat acagaactgg cgatcgttcg
     gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt gccgttttca tcatatttaa
     tcagcgactg atccacccag tcccagacga agccgccctg taaacgggga tactgacgaa
     acgcctgcca gtatttagcg aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc
     aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca ttttttgatg gaccatttcg
     gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg
     tggccgtggt gtcggctccg ccgccttcat actgcaccgg gcgggaagga tcgacagatt
     tgatccagcg atacagcgcg tcgtgattag cgccgtggcc tgattcattc cccagcgacc
     agatgatcac actcgggtga ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca
     tcgccggtag ccagcgcgga tcatcggtca gacgattcat tggcaccatg ccgtgggttt
     caatattggc ttcatccacc acatacaggc cgtagcggtc gcacagcgtg taccacagcg
     gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt gttctgcttc atcagcagga
     tatcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac
     ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc
     gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt
     gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt
     cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac
     cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta
     cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc
     tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat
     gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat
     aactgccgtc actccaacgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa
     atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc
     cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt
     cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg
     tgggaacaaa cggcggattg accgtaatgg gataggttac gttggtgtag atgggcgcat
     cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc
     actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc
     cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct
     gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca
     tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg
     ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa
     tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag
     cgagtgcctt cgactttttc gtgtccagtg tggcttgata tcatggagat aattaaaatg
     ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     aacctataaa tcatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
     ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggggat
     ccgagctcga gatctgcagc tggtaccatg gaattcgaag cttgtcgttg gatggaaagg
     aaaagagttc tacagggaaa cttggacccg cttcatggaa gacagcttcc ccattgttaa
     cgaccaagaa gtgatggatg ttttccttgt tgtcaacatg cgtcccacta gacccaaccg
     ttgttacaaa ttcctggccc aacacgctct gcgttgcgac cccgactatg tacctcatga
     cgtgattagg atcgtcgagc cttcatgggt gggcagcaac aacgagtacc gcatcagcct
     ggctaagaag ggcggcggct gcccaataat gaaccttcac tctgagtaca ccaactcgtt
     cgaacagttc atcgatcgtg tcatctggga gaacttctac aagcccatcg tttacatcgg
     taccgactct gctgaagagg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
     ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
     ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     aaataaagct agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
     cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
     cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
     ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
     ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
     aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
     gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
     gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
     ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
     acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag
     cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca
     cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc
     gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc
     gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat
     ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa
     tgtaagcaat tgtattgtaa agagattgtc tcaagctccg cacgccgata acaagccttt
     tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgact cgagttctat
     agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     ctaacgacaa tatgtccata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     caccgtcatc accgaaacgc gcgagaggaa agggcctcgt gatacgccta tttttatagg
     ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     aaccgcctct ccccgcgcgt tggccgattc attaatgcag gttaacctgg cttatcgaaa
     ttaatacgac tcactatagg gagaccggca gatcgatctg tcga
//