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ID   PBLUBCHISB preliminary; circular DNA; SYN; 10294 BP.
XX
AC   IG1043;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBacHisB - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBacHisB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA (pBlueBacIII)
CC   BR (pBlueBacHisA)(pBlueBacHisC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBlueBac
FT                   2. oligo, ATG/histidine repeats/enterokinase site
FT                   -> pBlueBacHisB 10294bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   misc_feature    0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 10294 BP; 2609 A; 2435 C; 2507 G; 2743 T; 0 other;
     gcagttcgtt gacgccttcc tccgtgtggc cgaacacgtc gagcgggtgg tcgatgacca
     gcggcgtgcc gcacgcgacg cacaagtatc tgtacaccga atgatcgtcg ggcgaaggca
     cgtcggcctc caagtggcaa tattggcaaa ttcgaaaata tatacagttg ggttgtttgc
     gcatatctat cgtggcgttg ggcatgtacg tccgaacgtt gatttgcatg caagccgaaa
     ttaaatcatt gcgattagtg cgattaaaac gttgtacatc ctcgctttta atcatgccgt
     cgattaaatc gcgcaatcga gtcaagtgat caaagtgtgg aataatgttt tctttgtatt
     cccgagtcaa gcgcagcgcg tattttaaca aactagccat cttgtaagtt agtttcattt
     aatgcaactt tatccaataa tatattatgt atcgcacgtc aagaattaac aatgcgcccg
     ttgtcgcatc tcaacacgac tatgatagag atcaaataaa gcgcgaatta aatagcttgc
     gacgcaacgt gcacgatctg tgcacgcgtt ccggcacgag ctttgattgt aataagtttt
     tacgaagcga tgacatgacc cccgtagtga caacgatcac gcccaaaaga actgccgact
     acaaaattac cgagtatgtc ggtgacgtta aaactattaa gccatccaat cgaccgttag
     tcgaatcagg accgctggtg cgagaagccg cgaagtatgg cgaatgcatc gtataacgtg
     tggagtccgc tcattagagc gtcatgttta gacaagaaag ctacatattt aattgatccc
     gatgatttta ttgataaatt gaccctaact ccatacacgg tattctacaa tggcggggtt
     ttggtcaaaa tttccggact gcgattgtac atgctgttaa cggctccgcc cactattaat
     gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcag
     atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     ctctagagtc gagatccccc tcgcccggtt attattattt ttgacaccag accaactggt
     aatggtagcg accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt
     cgccaccaat ccccatatgg aaaccgtcga tattcagcca tgtgccttct tccgcgtgca
     gcagatggcg atggctggtt tccatcagtt gctgttgact gtagcggctg atgttgaact
     ggaagtcgcc gcgccactgg tgtgggccat aattcaattc gcgcgtcccg cagcgcagac
     cgttttcgct cgggaagacg tacggggtat acatgtctga caatggcaga tcccagcggt
     caaaacaggc ggcagtaagg cggtcgggat agttttcttg cggccctaat ccgagccagt
     ttacccgctc tgctacctgc gccagctggc agttcaggcc aatccgcgcc ggatgcggtg
     tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg accactacca tcaatccggt
     aggttttccg gctgataaat aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa
     tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa caacgctgct tcggcctggt
     aatggcccgc cgccttccag cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac
     ttacgccaat gtcgttatcc agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca
     gttgtttttt atcgccaatc cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc
     aacgcttatt acccagctcg atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg
     cgtgggacgc ggcggggagc gtcacactga ggttttccgc cagacgccac tgctgccagg
     cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga
     gccagagttg cccggcgctc tccggctgcg gtagttcagg cagttcaatc aactgtttac
     cttgtggagc gacatccaga ggcacttcac cgcttgccag cggcttacca tccagcgcca
     ccatccagtg caggagctcg ttatcgctat gacggaacag gtattcgctg gtcacttcga
     tggtttgccc ggataaacgg aactggaaaa actgctgctg gtgttttgct tccgtcagcg
     ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat acagaactgg cgatcgttcg
     gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt gccgttttca tcatatttaa
     tcagcgactg atccacccag tcccagacga agccgccctg taaacgggga tactgacgaa
     acgcctgcca gtatttagcg aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc
     aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca ttttttgatg gaccatttcg
     gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg
     tggccgtggt gtcggctccg ccgccttcat actgcaccgg gcgggaagga tcgacagatt
     tgatccagcg atacagcgcg tcgtgattag cgccgtggcc tgattcattc cccagcgacc
     agatgatcac actcgggtga ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca
     tcgccggtag ccagcgcgga tcatcggtca gacgattcat tggcaccatg ccgtgggttt
     caatattggc ttcatccacc acatacaggc cgtagcggtc gcacagcgtg taccacagcg
     gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt gttctgcttc atcagcagga
     tatcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac
     ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc
     gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt
     gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt
     cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac
     cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta
     cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc
     tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat
     gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat
     aactgccgtc actccaacgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa
     atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc
     cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt
     cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg
     tgggaacaaa cggcggattg accgtaatgg gataggttac gttggtgtag atgggcgcat
     cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc
     actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc
     cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct
     gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca
     tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg
     ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa
     tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag
     cgagtgcctt cgactttttc gtgtccagtg tggcttgata tcatggagat aattaaaatg
     ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     aacctataaa tcatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
     ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgagctcga
     gatctgcagc tggtaccatg gaattcgaag cttgtcgttg gatggaaagg aaaagagttc
     tacagggaaa cttggacccg cttcatggaa gacagcttcc ccattgttaa cgaccaagaa
     gtgatggatg ttttccttgt tgtcaacatg cgtcccacta gacccaaccg ttgttacaaa
     ttcctggccc aacacgctct gcgttgcgac cccgactatg tacctcatga cgtgattagg
     atcgtcgagc cttcatgggt gggcagcaac aacgagtacc gcatcagcct ggctaagaag
     ggcggcggct gcccaataat gaaccttcac tctgagtaca ccaactcgtt cgaacagttc
     atcgatcgtg tcatctggga gaacttctac aagcccatcg tttacatcgg taccgactct
     gctgaagagg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca
     ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg ttattagtac
     atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt
     aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt
     tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt
     cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt
     tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc
     gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta
     tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaataaagct
     agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
     ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
     attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat
     ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga
     caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa
     tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg
     cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt
     ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg
     tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg
     tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa
     ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg
     tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt
     gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc
     ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag
     cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat
     tgtattgtaa agagattgtc tcaagctccg cacgccgata acaagccttt tcatttttac
     tacagcattg tagtggcgag acacttcgct gtcgtcgact cgagttctat agtgtcacct
     aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     tatgtccata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     accgaaacgc gcgagaggaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     ccccgcgcgt tggccgattc attaatgcag gttaacctgg cttatcgaaa ttaatacgac
     tcactatagg gagaccggca gatcgatctg tcga
//