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ID   PBLUBCHISC preliminary; circular DNA; SYN; 10302 BP.
XX
AC   IG1044;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBlueBacHisC - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   p2Bac from baculovirus
RC   p2Blue from p2Bac
RC   pBlueBac from AcNPV
RC   pBlueBac2 from AcNPV
RC   pBlueBacIII from AcNPV
RC   pBlueBacHisA from AcNPV
RC   pBlueBacHisB from AcNPV
RC   pBlueBacHisC from AcNPV
RC   pVL1392, pVL1393 from pAc series
RA   ;
RT   ;
RL   The Digest 5:2-2(1992).
XX
CC   NM (pBlueBacHisC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')(insect)
CC   CP ()
CC   FN (expression)(transfer)
CC   SE (color blue/white)
CC   PA (pBlueBacIII)
CC   BR (pBlueBacHisA)(pBlueBacHisB)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBlueBac
FT                   2. oligo, ATG/histidine repeats/enterokinase site
FT                   -> pBlueBacHisC 10302bp"
FT   promoter        0..0
FT                   /note="PRO AcMNPV polyhedrin gene"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   misc_feature    0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-BglII-PstI-NcoI-HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   transposon      0..0
FT                   /note="TRN AcMNPV"
FT   misc_binding    226..226
FT                   /note="SIT SphI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    2342..2342
FT                   /note="SIT XbaI"
FT   promoter        complement(0..0)
FT                   /note="PRO AcMNPV ETL gene"
XX
SQ   Sequence 10302 BP; 2611 A; 2437 C; 2509 G; 2745 T; 0 other;
     gcagttcgtt gacgccttcc tccgtgtggc cgaacacgtc gagcgggtgg tcgatgacca
     gcggcgtgcc gcacgcgacg cacaagtatc tgtacaccga atgatcgtcg ggcgaaggca
     cgtcggcctc caagtggcaa tattggcaaa ttcgaaaata tatacagttg ggttgtttgc
     gcatatctat cgtggcgttg ggcatgtacg tccgaacgtt gatttgcatg caagccgaaa
     ttaaatcatt gcgattagtg cgattaaaac gttgtacatc ctcgctttta atcatgccgt
     cgattaaatc gcgcaatcga gtcaagtgat caaagtgtgg aataatgttt tctttgtatt
     cccgagtcaa gcgcagcgcg tattttaaca aactagccat cttgtaagtt agtttcattt
     aatgcaactt tatccaataa tatattatgt atcgcacgtc aagaattaac aatgcgcccg
     ttgtcgcatc tcaacacgac tatgatagag atcaaataaa gcgcgaatta aatagcttgc
     gacgcaacgt gcacgatctg tgcacgcgtt ccggcacgag ctttgattgt aataagtttt
     tacgaagcga tgacatgacc cccgtagtga caacgatcac gcccaaaaga actgccgact
     acaaaattac cgagtatgtc ggtgacgtta aaactattaa gccatccaat cgaccgttag
     tcgaatcagg accgctggtg cgagaagccg cgaagtatgg cgaatgcatc gtataacgtg
     tggagtccgc tcattagagc gtcatgttta gacaagaaag ctacatattt aattgatccc
     gatgatttta ttgataaatt gaccctaact ccatacacgg tattctacaa tggcggggtt
     ttggtcaaaa tttccggact gcgattgtac atgctgttaa cggctccgcc cactattaat
     gaaattaaaa attccaattt taaaaaacgc agcaagagaa acatttgtat gaaagaatgc
     gtagaaggaa agaaaaatgt cgtcgacatg ctgaacaaca agattaatat gcctccgtgt
     ataaaaaaaa tattgaacga tttgaaagaa aacaatgtac cgcgcggcgg tatgtacagg
     aagaggttta tactaaactg ttacattgca aacgtggttt cgtgtgccaa gtgtgaaaac
     cgatgtttaa tcaaggctct gacgcatttc tacaaccacg actccaagtg tgtgggtgaa
     gtcatgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg ccaaaaaatg
     aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa tcctatttgt
     aattattgaa taataaaaca attataaatg ctaaatttgt tttttattaa cgatacaaac
     caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag ttataatcgc
     tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac aatataattt
     tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc tctttttcat
     ttttctcctc ataaaaatta acatagttat tatcgtatcc atatatgtat ctatcgtata
     gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt atagtaccgc
     tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct tcggagtgtg
     ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt ttgtacaata
     tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt agcagcaccg
     gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt tcacctccct
     tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc attgtaatga
     gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg ctgatatcag
     atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     ctctagagtc gagatccccc tcgcccggtt attattattt ttgacaccag accaactggt
     aatggtagcg accggcgctc agctggaatt ccgccgatac tgacgggctc caggagtcgt
     cgccaccaat ccccatatgg aaaccgtcga tattcagcca tgtgccttct tccgcgtgca
     gcagatggcg atggctggtt tccatcagtt gctgttgact gtagcggctg atgttgaact
     ggaagtcgcc gcgccactgg tgtgggccat aattcaattc gcgcgtcccg cagcgcagac
     cgttttcgct cgggaagacg tacggggtat acatgtctga caatggcaga tcccagcggt
     caaaacaggc ggcagtaagg cggtcgggat agttttcttg cggccctaat ccgagccagt
     ttacccgctc tgctacctgc gccagctggc agttcaggcc aatccgcgcc ggatgcggtg
     tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg accactacca tcaatccggt
     aggttttccg gctgataaat aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa
     tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa caacgctgct tcggcctggt
     aatggcccgc cgccttccag cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac
     ttacgccaat gtcgttatcc agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca
     gttgtttttt atcgccaatc cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc
     aacgcttatt acccagctcg atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg
     cgtgggacgc ggcggggagc gtcacactga ggttttccgc cagacgccac tgctgccagg
     cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga
     gccagagttg cccggcgctc tccggctgcg gtagttcagg cagttcaatc aactgtttac
     cttgtggagc gacatccaga ggcacttcac cgcttgccag cggcttacca tccagcgcca
     ccatccagtg caggagctcg ttatcgctat gacggaacag gtattcgctg gtcacttcga
     tggtttgccc ggataaacgg aactggaaaa actgctgctg gtgttttgct tccgtcagcg
     ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat acagaactgg cgatcgttcg
     gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt gccgttttca tcatatttaa
     tcagcgactg atccacccag tcccagacga agccgccctg taaacgggga tactgacgaa
     acgcctgcca gtatttagcg aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc
     aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca ttttttgatg gaccatttcg
     gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg
     tggccgtggt gtcggctccg ccgccttcat actgcaccgg gcgggaagga tcgacagatt
     tgatccagcg atacagcgcg tcgtgattag cgccgtggcc tgattcattc cccagcgacc
     agatgatcac actcgggtga ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca
     tcgccggtag ccagcgcgga tcatcggtca gacgattcat tggcaccatg ccgtgggttt
     caatattggc ttcatccacc acatacaggc cgtagcggtc gcacagcgtg taccacagcg
     gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt gttctgcttc atcagcagga
     tatcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac
     ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc
     gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt
     gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt
     cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac
     cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta
     cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc
     tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat
     gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat
     aactgccgtc actccaacgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa
     atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc
     cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt
     cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg
     tgggaacaaa cggcggattg accgtaatgg gataggttac gttggtgtag atgggcgcat
     cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc
     actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc
     cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct
     gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca
     tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg
     ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa
     tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag
     cgagtgcctt cgactttttc gtgtccagtg tggcttgata tcatggagat aattaaaatg
     ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     aacctataaa tcatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
     ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggatcc
     gacctcgaga tctgcagctg gtaccatgga attcgaagct tgtcgttgga tggaaaggaa
     aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg
     accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt
     gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg
     tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg
     ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg
     aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta
     ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg
     agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt
     attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
     cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
     aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
     ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
     atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
     cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
     cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
     ataaagctag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg
     tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca
     cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt
     ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt
     aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa
     tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc
     ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc
     tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc
     gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac
     aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg
     ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg
     ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc
     acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga
     ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct
     gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg
     taagcaattg tattgtaaag agattgtctc aagctccgca cgccgataac aagccttttc
     atttttacta cagcattgta gtggcgagac acttcgctgt cgtcgactcg agttctatag
     tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     aacgacaata tgtccatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     ccgtcatcac cgaaacgcgc gagaggaaag ggcctcgtga tacgcctatt tttataggtt
     aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     ccgcctctcc ccgcgcgttg gccgattcat taatgcaggt taacctggct tatcgaaatt
     aatacgactc actataggga gaccggcaga tcgatctgtc ga
//