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ID   PBLUE2KSP  preliminary; circular DNA; SYN; 2961 BP.
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AC   X52327;
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DT   10-MAY-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pBluescript II KS(+) - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2961
RC   pBluescript II KS(+)
RA   Thomas E.A.;
RT   ;
RL   Submitted (20-FEB-1990) on tape to the EMBL Data Library by:
RL   Thomas E.A., Stratagene Cloning Systems,
RL   11099 North Torrey Pines Rd., La Jolla, CA 92037, USA.
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RN   [2]
RC   pBluescript II from pBluescript
RC   pBluescript II KS, pBluescript II SK, from pBluescript
RC   pBluescript II KS+ from pBluescript KS+ & linker
RC   pBluescript II KS- from pBluescript KS- & linker
RC   pBluescript II SK+ from pBluescript SK+ & linker
RC   pBluescript II SK- from pBluescript SK- & linker
RA   Alting-Mees M.A., Short J.M.;
RT   "pBluescript II: gene mapping vectors";
RL   Nucleic Acids Res. 17:9494-9494(1989).
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CC   The KS designation indicates the polylinker is oriented such
CC   that beta-galactosidase (lacZ) transcription proceeds through the KpnI
CC   site first and the SacI site last.
CC   pBluescript KS(+) carries an F1 origin of replication,
CC   oriented such that transcription proceeds in
CC   the same direction as beta-galactosidase (lacZ) transcription.
CC   NM (pBluescript II KS+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli)
CC   CP ()
CC   FN (mapping gene)(expression)
CC   SE (color blue/white)
CC   PA (pUC19)(lambda ZAPII)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ 2964bp, 5' to T3 promoter
FT                   BssHII/BsePI linker 6bp gcgcgc
FT                   -> plasmid 2964bp
FT                   1. plasmid 2964bp, 3' to T7 promoter
FT                   BssHII/BsePI linker 6bp gcgcgc
FT                   -> pBluescript II KS+ 2961bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS (21 unique restriction sites)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
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SQ   Sequence 2961 BP; 747 A; 738 C; 755 G; 721 T; 0 other;
     ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
     ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
     gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca c
//