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ID   PBM272     preliminary; circular DNA; SYN; 8259 BP.
XX
AC   U03497;
XX
DT   17-NOV-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pBM272 - complete.
XX
KW   cloning vector; GAL promoter; centromere; inducible.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8259
RC   pBM272
RA   Stillman D.J.;
RT   ;
RL   Submitted (16-NOV-1993) by:
RL   Stillman D.J., Dept. of Cellular, Viral and Molecular Biology,
RL   University of Utah Medical Center, Salt Lake City UT 84132 USA.
XX
CC   NCBI gi: 416339
CC   NM (pBM272)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)(shuttle)(centromere)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. yeast, GAL promoter
FT                   3. yeast, centromere
FT                   -> pBM272 8259bp"
FT   misc_feature    0..0
FT                   /note="yeast centromere"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 8259 BP; 2294 A; 1882 C; 1829 G; 2254 T; 0 other;
     gaattcgaat tttcaaaaat tcttactttt tttttggatg gacgcaaaga agtttaataa
     tcatattaca tggcattacc accatataca tatccatata catatccata tctaatctta
     cttatatgtt gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt
     ggaactttca gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg
     ccgagcgggt gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg
     tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta
     caatactagc ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc
     ttcaaatgaa cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct
     tatttctggg gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa
     tgcaaaaact gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc
     ttattcaaat gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg
     tcaaggagaa aaaaccccgg atccaagctt gctctacgcc ggacgcatcg tggccggcat
     caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
     accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatcggg
     gggggggttt caattcaatt catcattttt tttttattct tttttttgat ttcggtttct
     ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
     acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
     cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
     tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
     catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
     actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
     cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
     caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
     gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
     ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
     tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
     tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
     tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     agcttcaatt taattatatc agttattacc cgggaatctc ggtcgtaatg atttttataa
     tgacgaaaaa aaaaaaattg gaaagaaaac cccccccccg cagcgttggg tcctggccac
     gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     tcattacccc catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga
     aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     tgatgagctt taccgcaggt gggccattct catgaagaat atcttgaatt tattgtcata
     ttactagttg gtgtggaagt ccatatatcg gtgatcaata tagtggttga catgctggct
     agtcaacatt gagccttttg atcatgcaaa tatattacgg tattttacaa tcaaatatca
     aacttaacta ttgactttat aacttattta ggtggtaaca ttcttataaa aaagaaaaaa
     attactgcaa aacagtacta gcttttaact tgtatcctag gttatctatg ctgtctcacc
     atagagaata ttacctattt cagaatgtat gtccatgatt cgccgggtaa atacatataa
     tacacaaatc tggcttaata aagtctataa tatatctcat aaagaagtgc taaattggct
     agtgctatat atttttaaga aaatttcttt tgactaagtc catatcgact ttgtaaaagt
     tcactttagc atacatatat tacacgagcc agaaattgta acttttgcct aaaatcacaa
     attgcaaaat ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt
     taaaatactt ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat
     gattaccgaa acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag
     cactacctag gagcggccaa aatgccgagg ctttcatagc ttaaactctt tacagaaaat
     aggcattata gatcagttcg agttttctta ttcttccttc cggttttatc gtcacagttt
     tacagtaaat aagtatcacc tcttagagtt aactatgaga taagcaagta tcatctcatt
     tcatttacct gaagtcgagt aaacagaaaa tccaattgtt gatgaacctc aatgacttag
     aactatctat cggcagatca tataaagagg atttaggtac ctagaggact gtacctggag
     tatatatata tatatatata tattatctca actatagtcc atagaggttt ctttcttgag
     gccttaaact gctaaagaat gatattggtg gaatgcaagc accaatctct cttctttcgt
     aactgttcat atacttcaaa ccaagaatgt aacgggcatt gacccatcca aaaccttcag
     tagctgcccc tttaaagtca gcaccttgat taccgtattc tgcttcaaca cgatgaggat
     ctgttcctct tgtgacatca tatttttcaa ccacaatacc attataatcg acaaaagcct
     ttgtcatcat gaaaagccat ctataagcta gcctattcgt tacagttaaa taaccataag
     aacggaggcc ttcccaagca agaatttgat ggggtgccca accaaatgga tagtcccatt
     gtctaattgg tctcgaaata gaaattgggc ctcgagaacg ctccgtacat gcagctaaac
     ctccaagcat ctctaacttg ggtagtgctt tctccaccat tttctgtgct tgctccttcg
     tggcaagtcc agcccataat gcccagaatg tagttgcgga ttcgtatgac gttctgtgct
     tgatttttgt gttgtagtca aagaaaaacc ccgactcgtc atcccacata tatttggtaa
     ttgatgaggc aacgctaatt atcaacatat agattgttat ctatctgcat gaacacgaaa
     tctttacttg acgacttgag gctgatggtg tttatgcaaa gaaaccactg tgtttaatat
     gtgtcactgt ttgatattac tgtcagcgta gaagataata gtaaaagcgg ttaataagtg
     tatttgagat aagtgtgata aagtttttac agcgaaaaga cgataaatac aagaaaatga
     ttacgaggat acggagagag gtatgtacat gtgtatttat atactaagct gccggcggtt
     gtttgcaaga ccgagaaaag gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga
     acattctctt caacaagttt gattccattg cggtgaaatg gtaaaagtca accccctgcg
     atgtatattt tcctgtacaa tcaatcaaaa agccaaatga tttagcatta tctttacatc
     ttgttatttt acagatttta tgtttagatc ttttatgctt gcttttcaaa aggcctgcag
     gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt
     tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct
     tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag
     tccaccagct aacataaaat gtaagctctg cctcgcgcgt ttcggtgatg acggtgaaaa
     cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
//