ID   PBP9       preliminary; circular DNA; SYN; 2868 BP.
XX
AC   IG9940;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pBP9 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   IR1 from phage f1
RC   phage IKe from E. coli
RC   pBP9 from pUR222 & M13mp9
RC   pIK200 from pBP9 & phage IKe
RC   pIK203 from pIK200
RC   pIK001 from pIK200, IKe ori
RC   pIKori-1, pIKori-2, pIKori-5, pIKori-7 from pIK001
RC   pIKori-2b(1), pIKori-2b(23) from pIKori-2
RA   Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT   "The gene II proteins of the filamentous phages IKe and Ff (M13,
RT   fd and f1) are not functionally interchangeable during viral
RT   strand replication";
RL   Nucleic Acids Res. 14:5067-5080(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pBP9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA (M13mp9)(pUR222)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUR222 remove PvuII-PvuII 295bp 1742..2037,
FT                   \ MCS/2405bp
FT                   2. M13mp9 PvuII-PvuII 463bp 5963..6426, MCS
FT                   -> pBP9 2868bp [unique SmaI, unique HindIII]"
FT   -               1..1741
FT                   /note="pUR222 1..1741 1741bp
FT                   PvuII = CAG^CTG"
FT   -               1742..2204
FT                   /note="M13mp9 5963..6425 463bp
FT                   PvuII = CAG^CTG"
FT   -               2205..2868
FT                   /note="pUR222 2037..2700 664bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2868 BP; 693 A; 732 C; 735 G; 708 T; 0 other;
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgatgccg
     ggcaagagca actcggtcgc gcgatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatcccta
     acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     gaactctgta gcaccgccta catacctcgt cctgctaatc ctgttaccag tggctgctgc
     cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gccattcgcc
     attcaggcta cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     gctgttgccc gtctcgctgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     acacaggaaa cagctatgac catgattacg ccaagcttgg ctgcaggtcg acggatcccc
     gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctggat taatgaatcg
     gccaacgcgc cgggagaggc ggtttgcgta ttgggcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagtaa tacactccgc tatcgctacg tgactgggtc atggctgcgc
     cccgacaccc gccaacaccc gctgacgcgc cctgatgggc ttgtctgctc ccggcatccg
     cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttaca
//