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ID   PBPV       preliminary; circular DNA; SYN; 12516 BP.
XX
AC   IG1045;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal/E. coli plasmid vector pBPV - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   BPV-1, BPV-2, BPV-3 from cow
RA   Lancaster W.D.;
RT   "Apparent lack of integration of bovine papillomavirus DNA in
RT   virus-induced equine and bovine tumor cells and virus-transformed
RT   mouse cells";
RL   Virology 108:251-255(1981).
XX
RN   [2]
RC   from pBPV-1(8-2)
RA   Law M.F., Lowy D.R., Dvoretzky I., Howley P.M.;
RT   "Mouse cells transformed by bovine papillomavirus contain only
RT   extrachromosomal viral DNA sequences";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:2727-2731(1981).
XX
RN   [3]
RC   pBPV-1(8-2), pBPV-1(9-1) from pBR322 & BPV-1
RC   pBPV-1(17-6), pBPV-1(17-2) from pBR322 & BPV-1 69%
RC   [pBPV69T from pBR322 & BPV-1 69%]
RA   Lowy D.R., Dvoretzky I., Shober R., Law M.F., Engel L., Howley P.M.;
RT   "In vitro tumorigenic transformation by a defined sub-genomic
RT   fragment of bovine papilloma virus DNA";
RL   Nature 287:72-74(1980).
XX
CC   Uses first ATG in inserted DNA.
CC   bovine papilloma virus (BPV1) is complete.
CC   no poison sequences.
CC   NM (pBPV)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli HB101)(mammal)(mouse C127)(mouse 3T3)
CC   HO (Fisher rat fibroblasts FR3T3)
CC   CP (20-150/cell)
CC   FN (expression shuttle)
CC   SE (transformation)
CC   PA (BPV1)(pML2d)(SV40)(MSV)(mouse metallothionein)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. cow
FT                   -> BPV-1 8000bp
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   2. BPV-1 7945bp 1..7945
FT                   -> pBPV-1(8-2) 12306bp
FT                   1. pBPV-1(8-2) 12400bp
FT                   2. MSV, LTR
FT                   3. mouse, metallothionein gene
FT                   4. SV40, early region
FT                   -> pBPV 12516bp"
FT   promoter        0..0
FT                   /note="PRO Moloney murine sarcoma virus (MSV)
FT                   long terminal repeat enhancer"
FT   promoter        0..0
FT                   /note="PRO mouse metallothionein I gene"
FT   misc_binding    1061..1076
FT                   /note="MCS unique XhoI-SacII-NotI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 mRNA splice site"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    4438..4438
FT                   /note="SIT unique HindIII"
FT   misc_binding    10151..10151
FT                   /note="SIT unique SalI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..<12472)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 12516 BP; 3381 A; 2852 C; 3005 G; 3278 T; 0 other;
     ctagaaactg ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagcggg
     gcaggaactg cttaccgcag atatcctgtt tgccccaatt cagctgttcc atctgttctt
     ggccctgagc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcagctg
     tctctctgtt cctgaccttg atctgaactt ctctattctc agttatgtat ttttcccatg
     ccttgcaaaa tggcgttact taagctagct tgccaaacct acggctgggg tctttcacgt
     ttatatctat gaggggaagg acccagagtg gggaagctgg gatcttggga acacgcttct
     ctacatggca ttgtctgcac ggtggagtcc ggatctgagc ttggcttggt ttttaaaacc
     agcctggagt agagcagatg ggttaaggtg agtgacccct cagccctgga cattcttaga
     tgagccccct caggagtaga gaataatgtt gagatgagtt ctgttggcta aaataatcaa
     ggctagtctt tataaaactg tctcctcttc tcctagcttc gatccagaga gagacctggg
     cggagctggt cgctgctcag gaactccagg aaaggagaag ctgaggttac cacgctgcga
     atgggtttac ggagatagct ggctttccgg ggtgagttct cgtaaactcc agagcagcga
     taggccgtaa tatcggggaa agcactatag ggacatgatg ttccacacgt cacatgggtc
     gtcctatccg agccagtcgt gccaaagggg cggtcccgct gtgcacactg gcgctccagg
     gagctctgca ctccgcccga aaagtgcgct cggctctgcc aggacgcggg gcgcgtgact
     atgcgtgggc tggagcaacc gcctgctggg tgcaaaccct ttgcgcccgg actcgtccaa
     cgactataaa gagggcaggc tgtcctctaa gcgtcaccac gacttcaacg tcctgagtac
     cttctcctca cttactccgt agctccagct tcaccagatc ctcgagccgc ggccgcttcg
     agggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     atcatgtctg gatccagagc tgtaacagca gggacccgcc ccagtatagg tgcgggcatt
     cctttagaca cccttgaaac tcttggggcc ttgcgtccag gggtgtatga ggacactgtg
     ctaccagagg cccctgcaat agtcactcct gatgctgttc ctgcagattc agggcttgat
     gccctgtcca taggtacaga ctcgtccacg gagaccctca ttactctgct agagcctgag
     ggtcccgagg acatagcggt tcttgagctg caacccctgg accgtccaac ttggcaagta
     agcaatgctg ttcatcagtc ctctgcatac cacgcccctc tgcagctgca atcgtccatt
     gcagaaacat ctggtttaga aaatattttt gtaggaggct cgggtttagg ggatacagga
     ggagaaaaca ttgaactgac atacttcggg tccccacgaa caagcacgcc ccgcagtatt
     gcctctaaat cacgtggcat tttaaactgg ttcagtaaac ggtactacac acaggtgccc
     acggaagatc ctgaagtgtt ttcatcccaa acatttgcaa acccactgta tgaagcagaa
     ccagctgtgc ttaagggacc tagtggacgt gttggactca gtcaggttta taaacctgat
     acacttacaa cacgtagcgg gacagaggtg ggaccacagc tacatgtcag gtactcattg
     agtactatac atgaagatgt agaagcaatc ccctacacag ttgatgaaaa tacacaggga
     cttgcattcg tacccttgca tgaagagcaa gcaggttttg aggagataga attagatgat
     tttagtgaga cacatagact gctacctcag aacacctctt ctacacctgt tggtagtggt
     gtacgaagaa gcctcattcc aactcaggaa tttagtgcaa cacggcctac aggtgttgta
     acctatggct cacctgacac ttactctgct agcccagtta ctgaccctga ttctacctct
     cctagtctag ttatcgatga cactactact acaccaatca ttataattga tgggcacaca
     gttgatttgt acagcagtaa ctacaccttg catccctcct tgttgaggaa acgaaaaaaa
     cggaaacatg cctaattttt tttgcagatg gcgttgtggc aacaaggcca gaagctgtat
     ctccctccaa cccctgtaag caaggtgctt tgcagtgaaa cctatgtgca aagaaaaagc
     attttttatc atgcagaaac ggagcgcctg ctaactatag gacatccata ttacccagtg
     tctatcgggg ccaaaactgt tcctaaggtc tctgcaaatc agtatagggt atttaaaata
     caactacctg atcccaatca atttgcacta cctgacagga ctgttcacaa cccaagtaaa
     gagcggctgg tgtgggcagt cataggtgtg caggtgtcca gagggcagcc tcttggaggt
     actgtaactg ggcaccccac ttttaatgct ttgcttgatg cagaaaatgt gaatagaaaa
     gtcaccaccc aaacaacaga tgacaggaaa caaacaggcc tagatgctaa gcaacaacag
     attctgttgc taggctgtac ccctgctgaa ggggaatatt ggacaacagc ccgtccatgt
     gttactgatc gtctagaaaa tggcgcctgc cctcctcttg aattaaaaaa caagcacata
     gaagatgggg atatgatgga aattgggttt ggtgcagcca acttcaaaga aattaatgca
     agtaaatcag atctacctct tgacattcaa aatgagatct gcttgtaccc agactacctc
     aaaatggctg aggacgctgc tggtaatagc atgttctttt ttgcaaggaa agaacaggtg
     tatgttagac acatctggac cagagggggc tcggagaaag aagcccctac cacagatttt
     tatttaaaga ataataaagg ggatgccacc cttaaaatac ccagtgtgca ttttggtagt
     cccagtggct cactagtctc aactgataat caaattttta atcggcccta ctggctattc
     cgtgcccagg gcatgaacaa tggaattgca tggaataatt tattgttttt aacagtgggg
     gacaatacac gtggtactaa tcttaccata agtgtagcct cagatggaac cccactaaca
     gagtatgata gctcaaaatt caatgtatac catagacata tggaagaata taagctagcc
     tttatattag agctatgctc tgtggaaatc acagctcaaa ctgtgtcaca tctgcaagga
     cttatgccct ctgtgcttga aaattgggaa ataggtgtgc agcctcctac ctcatcgata
     ttagaggaca cctatcgcta tatagagtct cctgcaacta aatgtgcaag caatgtaatt
      cctgcaaaag aagaccctta tgcagggttt aagttttgga acatagatct taaagaaaag
     ctttctttgg acttagatca atttcccttg ggaagaagat ttttagcaca gcaaggggca
     ggatgttcaa ctgtgagaaa acgaagaatt agccaaaaaa cttccagtaa gcctgcaaaa
     aaaaaaaaaa aataaaagct aagtttctat aaatgttctg taaatgtaaa acagaaggta
     agtcaactgc acctaataaa aatcacttaa tagcaatgtg ctgtgtcagt tgtttattgg
     aaccacaccc ggtacacatc ctgtccagca tttgcagtgc gtgcattgaa ttattgtgct
     ggctagactt catggcgcct ggcaccgaat cctgccttct cagcgaaaat gaataattgc
     tttgttggca agaaactaag catcaatggg acgcgtgcaa agcaccggcg gcggtagatg
     cggggtaagt actgaatttt aattcgacct atcccggtaa agcgaaagcg acacgctttt
     ttttcacaca tagcgggacc gaacacgtta taagtatcga ttaggtctat ttttgtctct
     ctgtcggaac cagaactggt aaaagtttcc attgcgtctg ggcttgtcta tcattgcgtc
     tctatggttt ttggaggatt agacggggcc accagtaatg gtgcatagcg gatgtctgta
     ccgccatcgg tgcaccgata taggtttggg gctccccaag ggactgctgg gatgacagct
     tcatattata ttgaatgggc gcataatcag cttaattggt gaggacaagc tacaagttgt
     aacctgatct ccacaaagta cgttgccggt cggggtcaaa ccgtcttcgg tgctcgaaac
     cgccttaaac tacagacagg tcccagccaa gtaggcggat caaaacctca aaaaggcggg
     agccaatcaa aatgcagcat tatattttaa gctcaccgaa accggtaagt aaagactatg
     tattttttcc cagtgaataa ttgttgttaa caataatcac accatcaccg ttttttcaag
     cgggaaaaaa tagccagcta actataaaaa gctgctgaca gaccccggtt ttcacatgga
     cctgaaacct tttgcaagaa ccaatccatt ctcagggttg gattgtctgt ggtgcagaga
     gcctcttaca gaagttgatg cttttaggtg catggtcaaa gactttcatg ttgtaattcg
     ggaaggctgt agatatggtg catgtaccat ttgtcttgaa aactgtttag ctactgaaag
     aagactttgg caaggtgttc cagtaacagg tgaggaagct gaattattgc atggcaaaac
     acttgatagg ctttgcataa gatgctgcta ctgtgggggc aaactaacaa aaaatgaaaa
     acatcggcat gtgcttttta atgagccttt ctgcaaaacc agagctaaca taattagagg
     acgctgctac gactgctgca gacatggttc aaggtccaaa tacccataga aacttggatg
     attcacctgc aggaccgttg ctgattttaa gtccatgtgc aggcacacct accaggtctc
     ctgcagcacc tgatgcacct gatttcagac ttccgtgcca tttcggccgt cctactagga
     agcgaggtcc cactacccct ccgctttcct ctcccggaaa actgtgtgca acagggccac
     gtcgagtgta ttctgtgact gtctgctgtg gaaactgcgg aaaagagctg acttttgctg
     tgaagaccag ctcgacgtcc ctgcttggat ttgaacacct tttaaactca gatttagacc
     tcttgtgtcc acgttgtgaa tctcgcgagc gtcatggcaa acgataaagg tagcaattgg
     gattcgggct tgggatgctc atatctgctg actgaggcag aatgtgaaag tgacaaagag
     aatgaggaac ccggggcagg tgtagaactg tctgtggaat ctgatcggta tgatagccag
     gatgaggatt ttgttgacaa tgcatcagtc tttcagggaa atcacctgga ggtcttccag
     gcattagaga aaaaggcggg tgaggagcag attttaaatt tgaaaagaaa agtattgggg
     agttcgcaaa acagcagcgg ttccgaagca tctgaaactc cagttaaaag acggaaatca
     ggagcaaagc gaagattatt tgctgaaaat gaagctaacc gtgttcttac gcccctccag
     gtacaggggg agggggaggg gaggcaagaa cttaatgagg agcaggcaat tagtcatcta
     catctgcagc ttgttaaatc taaaaatgct acagttttta agctggggct ctttaaatct
     ttgttccttt gtagcttcca tgatattacg aggttgttta agaatgataa gaccactaat
     cagcaatggg tgctggctgt gtttggcctt gcagaggtgt tttttgaggc gagtttcgaa
     ctcctaaaga agcagtgtag ttttctgcag atgcaaaaaa gatctcatga aggaggaact
     tgtgcagttt acttaatctg ctttaacaca gctaaaagca gagaaacagt ccggaatctg
     atggcaaaca cgctaaatgt aagagaagag tgtttgatgc tgcagccagc taaaattcga
     ggactcagcg cagctctatt ctggtttaaa agtagtttgt cacccgctac acttaaacat
     ggtgctttac ctgagtggat acgggcgcaa actactctga acgagagctt gcagaccgag
     aaattcgact tcggaactat ggtgcaatgg gcctatgatc acaaatatgc tgaggagtct
     aaaatagcct atgaatatgc tttggctgca ggatctgata gcaatgcacg ggctttttta
     gcaactaaca gccaagctaa gcatgtgaag gactgtgcaa ctatggtaag acactatcta
     agagctgaaa cacaagcatt aagcatgcct gcatatatta aagctaggtg caagctggca
     actggggaag gaagctggaa gtctatccta acttttttta actatcagaa tattgaatta
     attaccttta ttaatgcttt aaagctctgg ctaaaaggaa ttccaaaaaa aaactgttta
     gcatttattg gccctccaaa cacaggcaag tctatgctct gcaactcatt aattcatttt
     ttgggtggta gtgttttatc ttttgccaac cataaaagtc acttttggct tgcttcccta
     gcagatacta gagctgcttt agtagatgat gctactcatg cttgctggag gtactttgac
     acatacctca gaaatgcatt ggatggctac cctgtcagta ttgatagaaa acacaaagca
     gcggttcaaa ttaaagctcc acccctcctg gtaaccagta atattgatgt gcaggcagag
     gacagatatt tgtacttgca tagtcgggtg caaacctttc gctttgagca gccatgcaca
     gatgaatcgg gtgagcaacc ttttaatatt actgatgcag attggaaatc tttttttgta
     aggttatggg ggcgtttaga cctgattgac gaggaggagg atagtgaaga ggatggagac
     agcatgcgaa cgtttacatg tagcgcaaga aacacaaatg cagttgattg agaaaagtag
     tgataagttg caagatcata tactgtactg gactgctgtt agaactgaga acacactgct
     ttatgctgca aggaaaaaag gggtgactgt cctaggacac tgcagagtac cacactctgt
     agtttgtcaa gagagagcca agcaggccat tgaaatgcag ttgtctttgc aggagttaag
     caaaactgag tttggggatg aaccatggtc tttgcttgac acaagctggg accgatatat
     gtcagaacct aaacggtgct ttaagaaagg cgccagggtg gtagaggtgg agtttgatgg
     aaatgcaagc aatacaaact ggtacactgt ctacagcaat ttgtacatgc gcacagagga
     cggctggcag cttgcgaagg ctggggctga cggaactggg ctctactact gcaccatggc
     cggtgctgga cgcatttact attctcgctt tggtgacgag gcagccagat ttagtacaac
     agggcattac tctgtaagag atcaggacag agtgtatgct ggtgtctcat ccacctcttc
     tgattttaga gatcgcccag acggagtctg ggtcgcatcc gaaggacctg aaggagaccc
     tgcaggaaaa gaagccgagc cagcccagcc tgtctcttct ttgctcggct cccccgcctg
     cggtcccatc agagcaggcc tcggttgggt acgggacggt cctcgctcgc acccctacaa
     ttttcctgca ggctcggggg gctctattct ccgctcttcc tccaccccgt gcagggcacg
     gtaccggtgg acttggcatc aaggcaggaa gaagaggagc agtcgcccga ctccacagag
     gaagaaccag tgactctccc aaggcgcacc accaatgatg gattccacct gttaaaggca
     ggagggtcat gctttgctct aatttcagga actgctaacc aggtaaagtg ctatcgcttt
     cgggtgaaaa agaaccatag acatcgctac gagaactgca ccaccacctg gttcacagtt
     gctgacaacg gtgctgaaag acaaggacaa gcacaaatac tgatcacctt tggatcgcca
     agtcaaaggc aagactttct gaaacatgta ccactacctc ctggaatgaa catttccggc
     tttacagcca gcttggactt ctgatcactg ccattgcctt ttcttcatct gactggtgta
     ctatgccaaa tctatggttt ctattgttct tgggactagt tgctgcaatg caactgctgc
     tattactgtt cttactcttg ttttttcttg tatactggga tcattttgag tgctcctgta
     caggtctgcc cttttaatgc ctttacatca ctggctattg gctgtgtttt tactgttgtg
     tggatttgat ttgttttata tactgtatga agttttttca tttgtgcttg tattgctgtt
     tgtaagtttt ttactagagt ttgtattccc cctgctcaga ttttatatgg tttaagctgc
     agcaataaaa atgagtgcac gaaaaagagt aaaacgtgcc agtgcctatg acctgtacag
     gacatgcaag caagcgggca catgtccacc agatgtgata ccaaaggtag aaggagatac
     tatagcagat aaaattttga aatttggggg tcttgcaatc tacttaggag ggctaggaat
     aggaacatgg tctactggaa gggttgctgc aggtggatca ccaaggtaca caccactccg
     aacagcaggg tccacatcat cgcttgcatc aataggatcc tctacgccgg acgcatcgtg
     gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     aggcaggtag atgacgacca tcagggacag cttcaaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca
     tgtttgacag cttatcatcg ataagctgag atccgg
//