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ID   PBPV1      preliminary; circular DNA; SYN; 12310 BP.
XX
AC   IG9815;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pBPV-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pd1BPV69T(51-1) from pML2 & pBPV69T(43-1)
RC   pd1BPV69T(54-2) from pd1BPV69T(51-1)
RC   pML2d from pd1BPV69T(51-1)
RC   pdBPV-1(142-6) from pML2d & pBPV-1(8-2)
RA   Sarver N., Byrne J.C., Howley P.M.;
RT   "Transformation and replication in mouse cells of a bovine
RT   papillomavirus-pML2 plasmid vector that can be rescued in
RT   bacteria";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7147-7151(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pBPV-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(BPV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   fill in:fill in
FT                   2. BPV-1 7945bp 1..7945
FT                   -> pBPV-1(8-2) 12306bp"
FT   -               1..379
FT                   /note="pBR322 1..379 379bp
FT                   BamHI = G^GATC C"
FT   -               380..8324
FT                   /note="BPV-1 1..7945 7945bp
FT                   BamHI = G^GATCC"
FT   -               8325..12310
FT                   /note="pBR322 376..4361 3986bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 12310 BP; 3254 A; 2924 C; 3022 G; 3109 T; 1 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcg ttaacaataa tcacaccatc accgtttttt caagcgggaa
     aaaatagcca gctaactata aaaagctgct gacagacccc ggttttcaca tggacctgaa
     accttttgca agaaccaatc cattctcagg gttggattgt ctgtggtgca gagagcctct
     tacagaagtt gatgctttta ggtgcatggt caaagacttt catgttgtaa ttcgggaagg
     ctgtagatat ggtgcatgta ccatttgtct tgaaaactgt ttagctactg aaagaagact
     ttggcaaggt gttccagtaa caggtgagga agctgaatta ttgcatggca aaacacttga
     taggctttgc ataagatgct gctactgtgg gggcaaacta acaaaaaatg aaaaacatcg
     gcatgtgctt tttaatgagc ctttctgcaa aaccagagct aacataatta gaggacgctg
     ctacgactgc tgcagacatg gttcaaggtc caaataccca tagaaacttg gatgattcac
     ctgcaggacc gttgctgatt ttaagtccat gtgcaggcac acctaccagg tctcctgcag
     cacctgatgc acctgatttc agacttccgt gccatttcgg ccgtcctact aggaagcgag
     gtcccactac ccctccgctt tcctctcccg gaaaactgtg tgcaacaggg ccacgtcgag
     tgtattctgt gactgtctgc tgtggaaact gcggaaaaga gctgactttt gctgtgaaga
     ccagctcgac gtccctgctt ggatttgaac accttttaaa ctcagattta gacctcttgt
     gtccacgttg tgaatctcgc gagcgtcatg gcaaacgata aaggtagcaa ttgggattcg
     ggcttgggat gctcatatct gctgactgag gcagaatgtg aaagtgacaa agagaatgag
     gaacccgggg caggtgtaga actgtctgtg gaatctgatc ggtatgatag ccaggatgag
     gattttgttg acaatgcatc agtctttcag ggaaatcacc tggaggtctt ccaggcatta
     gagaaaaagg cgggtgagga gcagatttta aatttgaaaa gaaaagtatt ggggagttcg
     caaaacagca gcggttccga agcatctgaa actccagtta aaagacggaa atcaggagca
     aagcgaagat tatttgctga aaangaagct aaccgtgttc ttacgcccct ccaggtacag
     ggggaggggg aggggaggca agaacttaat gaggagcagg caattagtca tctacatctg
     cagcttgtta aatctaaaaa tgctacagtt tttaagctgg ggctctttaa atctttgttc
     ctttgtagct tccatgatat tacgaggttg tttaagaatg ataagaccac taatcagcaa
     tgggtgctgg ctgtgtttgg ccttgcagag gtgttttttg aggcgagttt cgaactccta
     aagaagcagt gtagttttct gcagatgcaa aaaagatctc atgaaggagg aacttgtgca
     gtttacttaa tctgctttaa cacagctaaa agcagagaaa cagtccggaa tctgatggca
     aacacgctaa atgtaagaga agagtgtttg atgctgcagc cagctaaaat tcgaggactc
     agcgcagctc tattctggtt taaaagtagt ttgtcacccg ctacacttaa acatggtgct
     ttacctgagt ggatacgggc gcaaactact ctgaacgaga gcttgcagac cgagaaattc
     gacttcggaa ctatggtgca atgggcctat gatcacaaat atgctgagga gtctaaaata
     gcctatgaat atgctttggc tgcaggatct gatagcaatg cacgggcttt tttagcaact
     aacagccaag ctaagcatgt gaaggactgt gcaactatgg taagacacta tctaagagct
     gaaacacaag cattaagcat gcctgcatat attaaagcta ggtgcaagct ggcaactggg
     gaaggaagct ggaagtctat cctaactttt tttaactatc agaatattga attaattacc
     tttattaatg ctttaaagct ctggctaaaa ggaattccaa aaaaaaactg tttagcattt
     attggccctc caaacacagg caagtctatg ctctgcaact cattaattca ttttttgggt
     ggtagtgttt tatcttttgc caaccataaa agtcactttt ggcttgcttc cctagcagat
     actagagctg ctttagtaga tgatgctact catgcttgct ggaggtactt tgacacatac
     ctcagaaatg cattggatgg ctaccctgtc agtattgata gaaaacacaa agcagcggtt
     caaattaaag ctccacccct cctggtaacc agtaatattg atgtgcaggc agaggacaga
     tatttgtact tgcatagtcg ggtgcaaacc tttcgctttg agcagccatg cacagatgaa
     tcgggtgagc aaccttttaa tattactgat gcagattgga aatctttttt tgtaaggtta
     tgggggcgtt tagacctgat tgacgaggag gaggatagtg aagaggatgg agacagcatg
     cgaacgttta catgtagcgc aagaaacaca aatgcagttg attgagaaaa gtagtgataa
     gttgcaagat catatactgt actggactgc tgttagaact gagaacacac tgctttatgc
     tgcaaggaaa aaaggggtga ctgtcctagg acactgcaga gtaccacact ctgtagtttg
     tcaagagaga gccaagcagg ccattgaaat gcagttgtct ttgcaggagt taagcaaaac
     tgagtttggg gatgaaccat ggtctttgct tgacacaagc tgggaccgat atatgtcaga
     acctaaacgg tgctttaaga aaggcgccag ggtggtagag gtggagtttg atggaaatgc
     aagcaataca aactggtaca ctgtctacag caatttgtac atgcgcacag aggacggctg
     gcagcttgcg aaggctgggg ctgacggaac tgggctctac tactgcacca tggccggtgc
     tggacgcatt tactattctc gctttggtga cgaggcagcc agatttagta caacagggca
     ttactctgta agagatcagg acagagtgta tgctggtgtc tcatccacct cttctgattt
     tagagatcgc ccagacggag tctgggtcgc atccgaagga cctgaaggag accctgcagg
     aaaagaagcc gagccagccc agcctgtctc ttctttgctc ggctcccccg cctgcggtcc
     catcagagca ggcctcggtt gggtacggga cggtcctcgc tcgcacccct acaattttcc
     tgcaggctcg gggggctcta ttctccgctc ttcctccacc ccgtgcaggg cacggtaccg
     gtggacttgg catcaaggca ggaagaagag gagcagtcgc ccgactccac agaggaagaa
     ccagtgactc tcccaaggcg caccaccaat gatggattcc acctgttaaa ggcaggaggg
     tcatgctttg ctctaatttc aggaactgct aaccaggtaa agtgctatcg ctttcgggtg
     aaaaagaacc atagacatcg ctacgagaac tgcaccacca cctggttcac agttgctgac
     aacggtgctg aaagacaagg acaagcacaa atactgatca cctttggatc gccaagtcaa
     aggcaagact ttctgaaaca tgtaccacta cctcctggaa tgaacatttc cggctttaca
     gccagcttgg acttctgatc actgccattg ccttttcttc atctgactgg tgtactatgc
     caaatctatg gtttctattg ttcttgggac tagttgctgc aatgcaactg ctgctattac
     tgttcttact cttgtttttt cttgtatact gggatcattt tgagtgctcc tgtacaggtc
     tgccctttta atgcctttac atcactggct attggctgtg tttttactgt tgtgtggatt
     tgatttgttt tatatactgt atgaagtttt ttcatttgtg cttgtattgc tgtttgtaag
     ttttttacta gagtttgtat tccccctgct cagattttat atggtttaag ctgcagcaat
     aaaaatgagt gcacgaaaaa gagtaaaacg tgccagtgcc tatgacctgt acaggacatg
     caagcaagcg ggcacatgtc caccagatgt gataccaaag gtagaaggag atactatagc
     agataaaatt ttgaaatttg ggggtcttgc aatctactta ggagggctag gaataggaac
     atggtctact ggaagggttg ctgcaggtgg atcaccaagg tacacaccac tccgaacagc
     agggtccaca tcatcgcttg catcaatagg atccagagct gtaacagcag ggacccgccc
     cagtataggt gcgggcattc ctttagacac ccttgaaact cttggggcct tgcgtccagg
     ggtgtatgag gacactgtgc taccagaggc ccctgcaata gtcactcctg atgctgttcc
     tgcagattca gggcttgatg ccctgtccat aggtacagac tcgtccacgg agaccctcat
     tactctgcta gagcctgagg gtcccgagga catagcggtt cttgagctgc aacccctgga
     ccgtccaact tggcaagtaa gcaatgctgt tcatcagtcc tctgcatacc acgcccctct
     gcagctgcaa tcgtccattg cagaaacatc tggtttagaa aatatttttg taggaggctc
     gggtttaggg gatacaggag gagaaaacat tgaactgaca tacttcgggt ccccacgaac
     aagcacgccc cgcagtattg cctctaaatc acgtggcatt ttaaactggt tcagtaaacg
     gtactacaca caggtgccca cggaagatcc tgaagtgttt tcatcccaaa catttgcaaa
     cccactgtat gaagcagaac cagctgtgct taagggacct agtggacgtg ttggactcag
     tcaggtttat aaacctgata cacttacaac acgtagcggg acagaggtgg gaccacagct
     acatgtcagg tactcattga gtactataca tgaagatgta gaagcaatcc cctacacagt
     tgatgaaaat acacagggac ttgcattcgt acccttgcat gaagagcaag caggttttga
     ggagatagaa ttagatgatt ttagtgagac acatagactg ctacctcaga acacctcttc
     tacacctgtt ggtagtggtg tacgaagaag cctcattcca actcaggaat ttagtgcaac
     acggcctaca ggtgttgtaa cctatggctc acctgacact tactctgcta gcccagttac
     tgaccctgat tctacctctc ctagtctagt tatcgatgac actactacta caccaatcat
     tataattgat gggcacacag ttgatttgta cagcagtaac tacaccttgc atccctcctt
     gttgaggaaa cgaaaaaaac ggaaacatgc ctaatttttt ttgcagatgg cgttgtggca
     acaaggccag aagctgtatc tccctccaac ccctgtaagc aaggtgcttt gcagtgaaac
     ctatgtgcaa agaaaaagca ttttttatca tgcagaaacg gagcgcctgc taactatagg
     acatccatat tacccagtgt ctatcggggc caaaactgtt cctaaggtct ctgcaaatca
     gtatagggta tttaaaatac aactacctga tcccaatcaa tttgcactac ctgacaggac
     tgttcacaac ccaagtaaag agcggctggt gtgggcagtc ataggtgtgc aggtgtccag
     agggcagcct cttggaggta ctgtaactgg gcaccccact tttaatgctt tgcttgatgc
     agaaaatgtg aatagaaaag tcaccaccca aacaacagat gacaggaaac aaacaggcct
     agatgctaag caacaacaga ttctgttgct aggctgtacc cctgctgaag gggaatattg
     gacaacagcc cgtccatgtg ttactgatcg tctagaaaat ggcgcctgcc ctcctcttga
     attaaaaaac aagcacatag aagatgggga tatgatggaa attgggtttg gtgcagccaa
     cttcaaagaa attaatgcaa gtaaatcaga tctacctctt gacattcaaa atgagatctg
     cttgtaccca gactacctca aaatggctga ggacgctgct ggtaatagca tgttcttttt
     tgcaaggaaa gaacaggtgt atgttagaca catctggacc agagggggct cggagaaaga
     agcccctacc acagattttt atttaaagaa taataaaggg gatgccaccc ttaaaatacc
     cagtgtgcat tttggtagtc ccagtggctc actagtctca actgataatc aaatttttaa
     tcggccctac tggctattcc gtgcccaggg catgaacaat ggaattgcat ggaataattt
     attgttttta acagtggggg acaatacacg tggtactaat cttaccataa gtgtagcctc
     agatggaacc ccactaacag agtatgatag ctcaaaattc aatgtatacc atagacatat
     ggaagaatat aagctagcct ttatattaga gctatgctct gtggaaatca cagctcaaac
     tgtgtcacat ctgcaaggac ttatgccctc tgtgcttgaa aattgggaaa taggtgtgca
     gcctcctacc tcatcgatat tagaggacac ctatcgctat atagagtctc ctgcaactaa
     atgtgcaagc aatgtaattc ctgcaaaaga agacccttat gcagggttta agttttggaa
     catagatctt aaagaaaagc tttctttgga cttagatcaa tttcccttgg gaagaagatt
     tttagcacag caaggggcag gatgttcaac tgtgagaaaa cgaagaatta gccaaaaaac
     ttccagtaag cctgcaaaaa aaaaaaaaaa ataaaagcta agtttctata aatgttctgt
     aaatgtaaaa cagaaggtaa gtcaactgca cctaataaaa atcacttaat agcaatgtgc
     tgtgtcagtt gtttattgga accacacccg gtacacatcc tgtccagcat ttgcagtgcg
     tgcattgaat tattgtgctg gctagacttc atggcgcctg gcaccgaatc ctgccttctc
     agcgaaaatg aataattgct ttgttggcaa gaaactaagc atcaatggga cgcgtgcaaa
     gcaccggcgg cggtagatgc ggggtaagta ctgaatttta attcgaccta tcccggtaaa
     gcgaaagcga cacgcttttt tttcacacat agcgggaccg aacacgttat aagtatcgat
     taggtctatt tttgtctctc tgtcggaacc agaactggta aaagtttcca ttgcgtctgg
     gcttgtctat cattgcgtct ctatggtttt tggaggatta gacggggcca ccagtaatgg
     tgcatagcgg atgtctgtac cgccatcggt gcaccgatat aggtttgggg ctccccaagg
     gactgctggg atgacagctt catattatat tgaatgggcg cataatcagc ttaattggtg
     aggacaagct acaagttgta acctgatctc cacaaagtac gttgccggtc ggggtcaaac
     cgtcttcggt gctcgaaacc gccttaaact acagacaggt cccagccaag taggcggatc
     aaaacctcaa aaaggcggga gccaatcaaa atgcagcatt atattttaag ctcaccgaaa
     ccggtaagta aagactatgt attttttccc agtgaataat tgttgatcct ctacgccgga
     cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     tcttcaagaa
//