back Return to this vector's summary.
ID   PBPV69T431 preliminary; circular DNA; SYN; 9494 BP.
XX
AC   ATCC37112;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pBPV69T (43-1) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   prI1 from pBR322 & insulin gene
RC   pBPV69T-rI1 from pBPV69T & prI1 & pBR322
RC   [pBPV69T(43-1) from pBR322 & BPV1 69% transforming region]
RA   Sarver N., Gruss P., Law M.F., Khoury G., Howley P.M.;
RT   "Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic
RT   cloning vector";
RL   Mol. Cell. Biol. 1:486-496(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Contains 69% subgenomic fragment cloned into the HindIII site of
CC   pBR322.
CC   This vector is suitable for introduction of foreign genes into mouse
CC   mammary tumor cell line C1271 (ATCC CRL 1616). (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBPV69T 43-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   HO (C127I cell line [ATCC CRL1616])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(BPV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI-HindIII 4015bp 376..4361..30
FT                   fill in:fill in
FT                   2. BPV-1 left end-ClaI 5475bp 1..5475, BPV-1 69% frag
FT                   \ BPV-1 = 7945bp
FT                   :blunt end
FT                   -> pBPV69T(43-1) 9490bp"
FT   -               1..4019
FT                   /note="pBR322 376..4361..33 4019bp
FT                   HindIII = A^AGCT T"
FT   -               4020..9494
FT                   /note="BPV-1 1..5475 5475bp
FT                   ClaI = AT^CGAT
FT                   BamHI = G^GATCC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 9494 BP; 2428 A; 2297 C; 2389 G; 2379 T; 1 other;
     gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaattct catgtttgac agcttatcat cgataagctg
     ttaacaataa tcacaccatc accgtttttt caagcgggaa aaaatagcca gctaactata
     aaaagctgct gacagacccc ggttttcaca tggacctgaa accttttgca agaaccaatc
     cattctcagg gttggattgt ctgtggtgca gagagcctct tacagaagtt gatgctttta
     ggtgcatggt caaagacttt catgttgtaa ttcgggaagg ctgtagatat ggtgcatgta
     ccatttgtct tgaaaactgt ttagctactg aaagaagact ttggcaaggt gttccagtaa
     caggtgagga agctgaatta ttgcatggca aaacacttga taggctttgc ataagatgct
     gctactgtgg gggcaaacta acaaaaaatg aaaaacatcg gcatgtgctt tttaatgagc
     ctttctgcaa aaccagagct aacataatta gaggacgctg ctacgactgc tgcagacatg
     gttcaaggtc caaataccca tagaaacttg gatgattcac ctgcaggacc gttgctgatt
     ttaagtccat gtgcaggcac acctaccagg tctcctgcag cacctgatgc acctgatttc
     agacttccgt gccatttcgg ccgtcctact aggaagcgag gtcccactac ccctccgctt
     tcctctcccg gaaaactgtg tgcaacaggg ccacgtcgag tgtattctgt gactgtctgc
     tgtggaaact gcggaaaaga gctgactttt gctgtgaaga ccagctcgac gtccctgctt
     ggatttgaac accttttaaa ctcagattta gacctcttgt gtccacgttg tgaatctcgc
     gagcgtcatg gcaaacgata aaggtagcaa ttgggattcg ggcttgggat gctcatatct
     gctgactgag gcagaatgtg aaagtgacaa agagaatgag gaacccgggg caggtgtaga
     actgtctgtg gaatctgatc ggtatgatag ccaggatgag gattttgttg acaatgcatc
     agtctttcag ggaaatcacc tggaggtctt ccaggcatta gagaaaaagg cgggtgagga
     gcagatttta aatttgaaaa gaaaagtatt ggggagttcg caaaacagca gcggttccga
     agcatctgaa actccagtta aaagacggaa atcaggagca aagcgaagat tatttgctga
     aaangaagct aaccgtgttc ttacgcccct ccaggtacag ggggaggggg aggggaggca
     agaacttaat gaggagcagg caattagtca tctacatctg cagcttgtta aatctaaaaa
     tgctacagtt tttaagctgg ggctctttaa atctttgttc ctttgtagct tccatgatat
     tacgaggttg tttaagaatg ataagaccac taatcagcaa tgggtgctgg ctgtgtttgg
     ccttgcagag gtgttttttg aggcgagttt cgaactccta aagaagcagt gtagttttct
     gcagatgcaa aaaagatctc atgaaggagg aacttgtgca gtttacttaa tctgctttaa
     cacagctaaa agcagagaaa cagtccggaa tctgatggca aacacgctaa atgtaagaga
     agagtgtttg atgctgcagc cagctaaaat tcgaggactc agcgcagctc tattctggtt
     taaaagtagt ttgtcacccg ctacacttaa acatggtgct ttacctgagt ggatacgggc
     gcaaactact ctgaacgaga gcttgcagac cgagaaattc gacttcggaa ctatggtgca
     atgggcctat gatcacaaat atgctgagga gtctaaaata gcctatgaat atgctttggc
     tgcaggatct gatagcaatg cacgggcttt tttagcaact aacagccaag ctaagcatgt
     gaaggactgt gcaactatgg taagacacta tctaagagct gaaacacaag cattaagcat
     gcctgcatat attaaagcta ggtgcaagct ggcaactggg gaaggaagct ggaagtctat
     cctaactttt tttaactatc agaatattga attaattacc tttattaatg ctttaaagct
     ctggctaaaa ggaattccaa aaaaaaactg tttagcattt attggccctc caaacacagg
     caagtctatg ctctgcaact cattaattca ttttttgggt ggtagtgttt tatcttttgc
     caaccataaa agtcactttt ggcttgcttc cctagcagat actagagctg ctttagtaga
     tgatgctact catgcttgct ggaggtactt tgacacatac ctcagaaatg cattggatgg
     ctaccctgtc agtattgata gaaaacacaa agcagcggtt caaattaaag ctccacccct
     cctggtaacc agtaatattg atgtgcaggc agaggacaga tatttgtact tgcatagtcg
     ggtgcaaacc tttcgctttg agcagccatg cacagatgaa tcgggtgagc aaccttttaa
     tattactgat gcagattgga aatctttttt tgtaaggtta tgggggcgtt tagacctgat
     tgacgaggag gaggatagtg aagaggatgg agacagcatg cgaacgttta catgtagcgc
     aagaaacaca aatgcagttg attgagaaaa gtagtgataa gttgcaagat catatactgt
     actggactgc tgttagaact gagaacacac tgctttatgc tgcaaggaaa aaaggggtga
     ctgtcctagg acactgcaga gtaccacact ctgtagtttg tcaagagaga gccaagcagg
     ccattgaaat gcagttgtct ttgcaggagt taagcaaaac tgagtttggg gatgaaccat
     ggtctttgct tgacacaagc tgggaccgat atatgtcaga acctaaacgg tgctttaaga
     aaggcgccag ggtggtagag gtggagtttg atggaaatgc aagcaataca aactggtaca
     ctgtctacag caatttgtac atgcgcacag aggacggctg gcagcttgcg aaggctgggg
     ctgacggaac tgggctctac tactgcacca tggccggtgc tggacgcatt tactattctc
     gctttggtga cgaggcagcc agatttagta caacagggca ttactctgta agagatcagg
     acagagtgta tgctggtgtc tcatccacct cttctgattt tagagatcgc ccagacggag
     tctgggtcgc atccgaagga cctgaaggag accctgcagg aaaagaagcc gagccagccc
     agcctgtctc ttctttgctc ggctcccccg cctgcggtcc catcagagca ggcctcggtt
     gggtacggga cggtcctcgc tcgcacccct acaattttcc tgcaggctcg gggggctcta
     ttctccgctc ttcctccacc ccgtgcaggg cacggtaccg gtggacttgg catcaaggca
     ggaagaagag gagcagtcgc ccgactccac agaggaagaa ccagtgactc tcccaaggcg
     caccaccaat gatggattcc acctgttaaa ggcaggaggg tcatgctttg ctctaatttc
     aggaactgct aaccaggtaa agtgctatcg ctttcgggtg aaaaagaacc atagacatcg
     ctacgagaac tgcaccacca cctggttcac agttgctgac aacggtgctg aaagacaagg
     acaagcacaa atactgatca cctttggatc gccaagtcaa aggcaagact ttctgaaaca
     tgtaccacta cctcctggaa tgaacatttc cggctttaca gccagcttgg acttctgatc
     actgccattg ccttttcttc atctgactgg tgtactatgc caaatctatg gtttctattg
     ttcttgggac tagttgctgc aatgcaactg ctgctattac tgttcttact cttgtttttt
     cttgtatact gggatcattt tgagtgctcc tgtacaggtc tgccctttta atgcctttac
     atcactggct attggctgtg tttttactgt tgtgtggatt tgatttgttt tatatactgt
     atgaagtttt ttcatttgtg cttgtattgc tgtttgtaag ttttttacta gagtttgtat
     tccccctgct cagattttat atggtttaag ctgcagcaat aaaaatgagt gcacgaaaaa
     gagtaaaacg tgccagtgcc tatgacctgt acaggacatg caagcaagcg ggcacatgtc
     caccagatgt gataccaaag gtagaaggag atactatagc agataaaatt ttgaaatttg
     ggggtcttgc aatctactta ggagggctag gaataggaac atggtctact ggaagggttg
     ctgcaggtgg atcaccaagg tacacaccac tccgaacagc agggtccaca tcatcgcttg
     catcaatagg atccagagct gtaacagcag ggacccgccc cagtataggt gcgggcattc
     ctttagacac ccttgaaact cttggggcct tgcgtccagg ggtgtatgag gacactgtgc
     taccagaggc ccctgcaata gtcactcctg atgctgttcc tgcagattca gggcttgatg
     ccctgtccat aggtacagac tcgtccacgg agaccctcat tactctgcta gagcctgagg
     gtcccgagga catagcggtt cttgagctgc aacccctgga ccgtccaact tggcaagtaa
     gcaatgctgt tcatcagtcc tctgcatacc acgcccctct gcagctgcaa tcgtccattg
     cagaaacatc tggtttagaa aatatttttg taggaggctc gggtttaggg gatacaggag
     gagaaaacat tgaactgaca tacttcgggt ccccacgaac aagcacgccc cgcagtattg
     cctctaaatc acgtggcatt ttaaactggt tcagtaaacg gtactacaca caggtgccca
     cggaagatcc tgaagtgttt tcatcccaaa catttgcaaa cccactgtat gaagcagaac
     cagctgtgct taagggacct agtggacgtg ttggactcag tcaggtttat aaacctgata
     cacttacaac acgtagcggg acagaggtgg gaccacagct acatgtcagg tactcattga
     gtactataca tgaagatgta gaagcaatcc cctacacagt tgatgaaaat acacagggac
     ttgcattcgt acccttgcat gaagagcaag caggttttga ggagatagaa ttagatgatt
     ttagtgagac acatagactg ctacctcaga acacctcttc tacacctgtt ggtagtggtg
     tacgaagaag cctcattcca actcaggaat ttagtgcaac acggcctaca ggtgttgtaa
     cctatggctc acctgacact tactctgcta gcccagttac tgaccctgat tctacctctc
     ctagtctagt tatc
//