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ID   PBR322     preliminary; circular DNA; SYN; 4361 BP.
AC   J01749; K00005; L08654; M10282; M10283; M10286; M10356; M10784;
AC   M10785; M10786; M33694; V01119; VB0001; ATCC31344; ATCC37017;
DT   09-JUN-1982 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pBR322 - complete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-3; 3259-4361
RC   pSF2124 from ColE1 & R1
RC   pBR312 from pSF2124
RC   plasmid from pBR312
RC   pBR322 from plasmid
RA   Sutcliffe J.G.;
RT   "Nucleotide sequence of the ampicillin resistance gene of
RT   Escherichia coli plasmid pBR322";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:3737-3741(1978).
RN   [2]
RP   1-4363
RC   pac163 from pBR322
RC   pACR1 from pBR322
RA   Sutcliffe J.G.;
RT   "Complete nucleotide sequence of the Escherichia coli plasmid
RT   pBR322";
RL   Cold Spring Harb. Symp. Quant. Biol. 43:77-90(1979).
RN   [3]
RP   1500-2300
RC   pOX30 from pBR322 & pRS15 & pRS2
RA   Reed R.R., Young R.A., Steitz J.A., Grindley N.D.F., Guyer M.;
RT   "Revised sequence of the tetracycline-resistance gene of pBR322";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:4882-4886(1979).
RN   [4]
RP   2207-2265
RC   pBR326 from pBR322
RC   pBR3286, pBR3287 from pBR328
RA   Covarrubias L., Cervantes L., Covarrubias A., Soberon X., Vichido I.,
RA   Blanco A., Kupersztoch-Portnoy Y.M., Bolivar F.;
RT   "Construction and characterization of new cloning vehicles. V.
RT   Mobilization and coding properties of pBR322 and several deletion
RT   derivatives including pBR327 and pBR328";
RL   Gene 13:25-35(1981).
RN   [5]
RP   2000-2500
RC   f1YB-5, f1YB-1 from pBR322 & R229 & linker
RC   f1YBd9 from f1YB-5
RA   Marians K.J., Soeller W., Zipursky S.L.;
RT   "Maximal limits of the Escherichia coli replication factor Y
RT   effector site sequences in pBR322 DNA";
RL   J. Biol. Chem. 257:5656-5662(1982).
RN   [6]
RP   1-80; 4151-4229; 4349-4361
RC   from pBR322, amp gene
RA   Brosius J., Cate R.L., Perlmutter A.P.;
RT   "Precise location of two promoters for the beta-lactamase gene of
RT   pBR322 : S1 mapping of ribonucleic acid isolated from Escherichia
RT   coli or synthesized in vitro";
RL   J. Biol. Chem. 257:9205-9210(1982).
RN   [7]
RP   4241-4343
RC   from pBR322
RA   Van Dyke M.W., Hertzberg R.P., Dervan P.B.;
RT   "Map of distamycin, netropsin, and actinomycin binding sites on
RT   heterogeneous DNA: DNA cleavage-inhibition patterns with
RT   methidiumpropyl-EDTA.Fe(II)";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:5470-5474(1982).
RN   [8]
RP   584-709
RC   pKP10, pKP11, pKP12 from pBR322
RC   pKP25 from pBR322
RC   pKP30 from pBR322
RA   Peden K.W.C., Nathans D.;
RT   "Local mutagenesis within deletion loops of DNA heteroduplexes";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7214-7217(1982).
RN   [9]
RP   373-649 or 1-1276
RC   from pBR322, tet gene
RA   Peden K.W.;
RT   "Revised sequence of the tetracycline-resistance gene of pBR322";
RL   Gene 22:277-280(1983).
RN   [10]
RP   132-181
RC   from pBR322, SceI site
RC   from phage phiX174, SceI site
RC   from phage fd, SceI site
RA   Watabe H.O., Iino T., Kaneko T., Shibata T., Ando T.;
RT   "A new class of site-specific endo-deoxyribonucleases: Endo.Sce I
RT   isolated from a eukaryote, Saccharomyces cerevisiae";
RL   J. Biol. Chem. 258:4663-4665(1983).
RN   [11]
RP   368-581
RC   from pXf3
RA   Livneh Z.;
RT   "Directed mutagenesis method for analysis of mutagen specificity:
RT   Application to ultraviolet-induced mutagenesis";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:237-241(1983).
RN   [12]
RP   2627-2682; 2781-2828
RC   from pBR322
RA   Mascharak P.K., Sugiura Y., Kuwahara J., Suzuki T., Lippard S.J.;
RT   "Alteration and activation of sequence-specific cleavage of DNA by
RT   bleomycin in the presence of the antitumor drug
RT   cis-diamminedichloroplatinum(II)";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:6795-6798(1983).
RN   [13]
RP   4276-4336
RC   from pBR322
RA   Schultz P.G., Dervan P.B.;
RT   "Sequence-specific double-stranded cleavage of DNA by
RT   penta-N-methylpyrrolecarboxamide-EDTA.Fe(II)";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:6834-6837(1983).
RN   [14]
RP   518-528
RC   pBR322 from pBR312
RA   Sutcliffe J.G.;
RT   ;
RL   Unpublished (1984).
RN   [15]
RP   2395-2495
RC   pBF120 from pBR322 & dnaA gene & linker
RC   pBF1209 from pBF120 & linker
RC   pBF1363 from pBF120 & linker
RA   Fuller R.S., Funnell B.E., Kornberg A.;
RT   "The dnaA protein complex with the E. coli chromosomal replication
RT   origin (oriC) and other DNA sites";
RL   Cell 38:889-900(1984).
RN   [16]
RP   2729-2731 or 1-4363
RC   from pBR322, oligos
RA   Lathe R., Kieny M.P., Skory S., Lecocq J.P.;
RT   "Laboratory methods linker tailing: Unphosphorylated linker
RT   oligonucleotides for joining DNA termini";
RL   DNA 3:173-182(1984).
RN   [17]
RP   1-4363
RC   pJRD158 from pBR322 & pBR327
RA   Huesterspreute M., Davison J.;
RT   "Restriction site bank vectors. II. DNA sequence analysis of
RT   plasmid pJRD158";
RL   DNA 3:259-268(1984).
RN   [18]
RP   2113-2186; 2348-2415
RC   f1pas- series from phage f1 & phage phiX174
RC   f1mApas- series from phage f1 & phage phiX174
RC   from pBR322 & f1mApas- series, primosome assembly site/Y factor
RA   Abarzua P.I., Soeller W., Marians K.J.;
RT   "Mutational analysis of the primosome assembly sites: I. Distinct
RT   classes of mutants in the pBR322 Escherichia coli factor Y DNA
RT   effector sequences";
RL   J. Biol. Chem. 259:14286-14292(1984).
RN   [19]
RP   2348-2415
RC   f1m or f1R2295B from R229
RC   from pBR322 & f1m, primosome assembly site/Y factor
RA   Soeller W., Abarzua P.I., Marians K.J.;
RT   "Mutational analysis of primosome assembly sites: II. Role of
RT   secondary structure in the formation of active sites";
RL   J. Biol. Chem. 259:14293-14300(1984).
RN   [20]
RP   1-4361
RC   from pBR322
RA   Van Dyke M.M., Dervan P.B.;
RT   "Echinomycin binding sites on DNA";
RL   Science 225:1122-1127(1984).
RN   [21]
RP   1-4361
RC   from pBR322
RA   Watson N.;
RT   "A new revision of the sequence of plasmid pBR322";
RL   Gene 70:399-403(1988).
RN   [22]
RC   from pBR322
RA   Crea R., Kraszewski A., Hirose T., Itakura K.;
RT   "Chemical synthesis of genes for human insulin";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:5765-5769(1978).
RN   [23]
RC   pBB101 from pBR322 & human insulin beta chain gene
RC   pBH1, pBH2, pBH3 from pBR322 & human insulin beta chain gene
RC   pA10, pA11 from pBR322 & human insulin alpha chain gene
RC   pIA1 from pA11 & lambda plac5
RC   pIB1 from pBH1 & lambda plac5
RA   Goeddel D.V., Kleid D.G., Bolivar F., Heyneker H.L., Yansura D.G.,
RA   Crea R., Hirose T., Kraszewski A., Itakura K., Riggs A.D.;
RT   "Expression in Escherichia coli of chemically synthesized genes for
RT   human insulin";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:106-110(1979).
RN   [24]
RC   pBR318 from pBR313
RC   pBR320 from pBR313
RC   pBR321, pBR322 from pBR318 & pBR320
RC   [ColE1::Tn7 from ColE1 & R483]
RA   Bolivar F., Rodriguez R.L., Greene P.J., Betlach M.C.,
RA   Heyneker H.L., Boyer H.W.;
RT   "Construction and characterization of new cloning vehicles
RT   II. A multipurpose cloning system";
RL   Gene 2:95-113(1977).
RN   [25]
RC   [pMB1 from ColE1]
RC   pMB3 from pMB1 & Tn3, amp gene
RC   pMB8 from pMB3
RC   pMB9 from pMB8 & pSC101, tet gene
RC   pBR312 from pMB9 & pSF2124, Tn3
RC   pBR313 from pBR312
RC   pBR318, pBR320 from pBR313
RC   pBR322 from pBR318 & pBR320
RA   Rodriguez R.L., Tait R.C.;
RT   ;
RL   Recombinant DNA Technology An Introduction 0:187-187(1983).
RL   Rodriguez R.L., Tait R.C., Addison-Wesley, Reading MA.
RN   [26]
RC   pOX38 from F factor, Tn1000 or gammadelta tra operon
RC   F delta(0-15) from F factor
RC   pOX67 from F delta(0-15) & Tn1000 or gammadelta
RC   pOX119 from pOX38 & Tn1000 or gammadelta
RA   Guyer M.S., Reed R.R., Steitz J.A., Low K.B.;
RT   "Identification of a sex-factor-affinity site in E. coli as
RT   gammadelta";
RL   Cold Spring Harb. Symp. Quant. Biol. 45:135-140(1980).
RN   [27]
RC   pOX16 from pBR322 & Tn1000 or gammadelta
RA   Guyer M.S.;
RT   "Uses of the transposon gammadelta in the analysis of cloned genes";
RL   Meth. Enzymol. 101:362-369(1983).
RN   [28]
RC   pOX13, pOX14, pOX15, pOX16 from pBR322 & Tn1000 or gammadelta
RA   Guyer M.S.;
RT   "The gammadelta sequence of F is an insertion sequence";
RL   J. Mol. Biol. 126:347-365(1978).
RN   [29]
RC   pRS2 from F factor & gammadelta
RC   pRS5 from pSC101 & F factor
RC   pRS15 from F factor & gammadelta
RC   pRS21 from pSC101 & F factor
RA   Skurray R.A., Nagaishi H., Clark A.J.;
RT   "Molecular cloning of DNA from F sex factor of Escherichia coli
RT   K-12";
RL   Proc. Natl. Acad. Sci. U.S.A. 73:64-68(1976).
RN   [30]
RC   M13oriC26 from M13 & E.coli oriC
RC   M13oriC26delta221 from M13oriC26
RC   pSY317 from E. coli oriC
RA   Fuller R.S., Kaguni J.M., Kornberg A.;
RT   "Enzymatic replication of the origin of the Escherichia coli
RT   chromosome";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:7370-7374(1981).
RN   [31]
RC   pMAPCdSG10 from pBR322
RC   pTAO7 from E. coli oriC & linker & pMAPCdSG10
RA   Ogawa T., Pickett G.G., Kogoma T., Kornberg A.;
RT   "RNase H confers specificity in the dnaA-dependent initiation of
RT   replication at the unique origin of the Escherichia coli
RT   chromosome in vivo and in vitro";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:1040-1044(1984).
RN   [32]
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
CC   Size is 4363 for Boehringer, BRL, Promega, Sigma.
CC   Size is 4362 for USB.
CC   Promoter P1 is artificially created by the ligation of two
CC   different DNA fragments to create pBR322.
CC   Promoter P2 in the same region as P1, but it is on the opposite
CC   strand and initiates transcription in the direction of the
CC   tetracycline resistance gene.
CC   Promoter P3 is the natural promoter for the beta-lactamase gene.
CC   Medium is 1227 LB plus ampicillin.
CC   The circular sequence is numbered such that 0 is the middle
CC   of the unique EcoRI site and the count increases first through the
CC   tet genes, the pMB1 material, and finally through the Tn3 region.
CC   NCBI gi: 58257
CC   NM (pBR322)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Boehringer)(BRL)(CLONTECH)(NEB)(Pharmacia)(Promega)(Sigma)
CC   SP (USB)(Worthington)(ATCC)
CC   HO (E.coli HB101)(E.coli C600)(E.coli RR1)(E.coli)
CC   CP ()
CC   FN (cloning general)
CC   SE ()
CC   PA (pSC101)
CC   BR ()
CC   OF (M13mp9)(pAA3.7X)(pACYC177)(pAT153)(pBR325)(pBR327)
CC   OF (pBR328)(pCKSP6)(pEX1)(pEX2)(pEX3)(pGEM-1)(pGEM-2)
CC   OF (pGEM-3)(pGEM-4)(pHC79)(pi-AN7)(pi-VX)(pJRD158)
CC   OF (pKK223-3)(pKM1)(pKM2)(pKO-1)(pKO-2)(pKTH605)(pKTH606)
CC   OF (pLBU3)(pMBL1)(pMBL604)(pMC1511)(pMC1871)(pRSVneo)
CC   OF (pSP18)(pSP19)(pSP64)(pSP65)(pSP6-T3)(pSP6-T7-19)
CC   OF (pSV2cat)(pT712)(pT713)(pT7T3-18)(pT7T3-19)(pT7T3alpha-18)
CC   OF (pT7T3alpha-19)(pTrS3)(pUC7)(pUR222)(pUR278)(pUR288)
CC   OF (pUR289)(pUR290)(pUR291)(pUR292)(pV34)(YEp24)(YIp5)(YRp17)(pREV-T)
CC   OF (pHS102)(pEMP)(pTCT)(pSVC)(ppi12.20)(ppi25.1)(pS12.20)(pS25.1)
CC   OF (pPH54)(pPH507)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR313 PstI-PstI, amp/tet/ori
FT                   -> pBR318 [unique PstI]
FT                   1. pBR313 EcoRI-EcoRI, amp/tet/ori
FT                   -> pBR320 [unique PstI]
FT                   1. pBR318 small PstI-HpaI, tet gene
FT                   2. pBR320 largest PstI-HincII, ori/amp gene
FT                   -> pBR322 4361bp"
FT   misc_binding    23..23
FT                   /note="SIT unique ClaI"
FT   promoter        complement(27..33)
FT                   /note="PRO E. coli promoter P1 [6]"
FT   misc_binding    29..29
FT                   /note="SIT unique HindIII"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   promoter        43..49
FT                   /note="PRO E. coli promoter P2 [6]"
FT   misc_binding    185..185
FT                   /note="SIT unique EcoRV"
FT   misc_binding    229..229
FT                   /note="SIT unique NheI"
FT   misc_binding    375..375
FT                   /note="SIT unique BamHI"
FT   misc_binding    562..562
FT                   /note="SIT unique SphI"
FT   misc_binding    622..622
FT                   /note="SIT unique EcoNI"
FT   misc_binding    651..651
FT                   /note="SIT unique SalI"
FT   misc_binding    939..939
FT                   /note="SIT unique XmaIII"
FT   misc_binding    972..972
FT                   /note="SIT unique NruI"
FT   misc_binding    1063..1063
FT                   /note="SIT unique BspMI"
FT   misc_binding    1353..1353
FT                   /note="SIT unique BsmI"
FT   misc_binding    1369..1369
FT                   /note="SIT unique StyI"
FT   misc_binding    1425..1425
FT                   /note="SIT unique AvaI"
FT   misc_binding    1444..1444
FT                   /note="SIT unique BalI"
FT   repeat_unit     complement(1515..1519)
FT                   /note="gamma-delta insertion target sequence;
FT                   direct repeat"
FT   misc_binding    1664..1664
FT                   /note="SIT unique BspMII"
FT   misc_binding    1668..1668
FT                   /note="SIT unique MamI"
FT   repeat_unit     complement(1788..1792)
FT                   /note="gamma-delta insertion target sequence;
FT                   direct repeat"
FT   RBS             1905..1909
FT                   /note="RBS Shine-Dalgarno sequence"
FT   CDS             1915..2106
FT                   /note="REP E. coli ROP gene"
FT   misc_feature    2011..2167
FT                   /note="H-strand Y effector site [5]"
FT   misc_binding    2066..2066
FT                   /note="SIT unique PvuII"
FT   misc_binding    2124..2124
FT                   /note="SIT unique Esp3I"
FT   misc_binding    2164..2164
FT                   /note="SIT double DrdI"
FT   misc_binding    2219..2219
FT                   /note="SIT unique Tth111I"
FT   misc_binding    2227..2227
FT                   /note="SIT unique BsaAI"
FT   repeat_unit     complement(2245..2249)
FT                   /note="gamma-delta insertion target sequence;
FT                   direct repeat"
FT   misc_binding    2246..2246
FT                   /note="SIT unique Bst1107I"
FT   misc_binding    2297..2297
FT                   /note="SIT unique NdeI"
FT   misc_feature    complement(2351..2414)
FT                   /note="L-strand Y effector site [5]"
FT   misc_binding    2353..2353
FT                   /note="SIT double EarI"
FT   misc_binding    2475..2475
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(2535..2535)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2577..2577
FT                   /note="SIT double DrdI"
FT   misc_binding    2886..2886
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(3293..4153)
FT                   /note="ANT E. coli beta-lactamase gene (bla E-286)
FT                   ampicillin resistance gene (apr/amp)"
FT   mat_peptide     complement(3296..4153)
FT                   /note="ANT E. coli beta-lactamase gene (bla E-286)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3539..3539
FT                   /note="SIT unique AseI"
FT   misc_binding    3609..3609
FT                   /note="SIT unique PstI"
FT   misc_binding    3735..3735
FT                   /note="SIT unique PvuI"
FT   misc_binding    3846..3846
FT                   /note="SIT unique ScaI"
FT   misc_binding    4157..4157
FT                   /note="SIT double EarI"
FT   RBS             complement(4161..4165)
FT                   /note="RBS Shine-Dalgarno sequence"
FT   misc_binding    4170..4170
FT                   /note="SIT unique SspI"
FT   promoter        complement(4188..4194)
FT                   /note="PRO E. coli promoter P3 [3]"
FT   misc_binding    4200..4200
FT                   /note="SIT unique BsmAI"
FT   misc_binding    4286..4286
FT                   /note="SIT unique AatII"
FT   misc_binding    4361..4361
FT                   /note="SIT unique EcoRI"
SQ   Sequence 4361 BP; 983 A; 1210 C; 1134 G; 1034 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a