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ID   PBR322SV40 preliminary; circular DNA; SYN; 3102 BP.
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AC   IG9811;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pBR322-SV40 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV
RC   pBR322-SV40 from pBR322 & SV40 early prom/splice acceptor/ori
RC   pL1 from pBR322-SV40
RC   [pcD-X from pcDV1]
RC   pcD-dhfr from pcDV1 & pL1 & DHFR gene
RC   pcD-alphaG from pcDV1 & pL1 & alpha-globin gene
RA   Okayama H., Berg P.;
RT   "A cDNA cloning vector that permits expression of cDNA inserts
RT   in mammalian cells";
RL   Mol. Cell. Biol. 3:280-289(1983).
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RN   [2]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV
RC   pBR322-SV40 from pBR322 & SV40 early prom/splice acceptor/ori
RC   pL1 from pBR322-SV40
RA   Okayama H., Berg P.;
RT   "High efficiency cloning of full-length cDNA";
RL   Mol. Cell. Biol. 2:161-170(1982).
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RN   [3]
RC   pBR322-SV40 from pBR322 & SV40
RA   Subramani S.;
RT   ;
RL   Unpublished (1982).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pBR322-SV40)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ tet/2324bp
FT                   2. SV40 PvuII-HindIII 774bp 273..1047
FT                   \ late polyA/786bp/0.71 to 0.86
FT                   -> pBR322-SV40 3101bp"
FT   -               1..2324
FT                   /note="pBR322 2067..4361..29 2324bp
FT                   HindIII = A^AGCTT"
FT   -               2325..3102
FT                   /note="SV40 273..1050 778bp complement
FT                   PvuII = CAG^CTG"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
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SQ   Sequence 3102 BP; 836 A; 774 C; 731 G; 761 T; 0 other;
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagcttg agaaatggca
     ttaaaaagtg ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt
     tgtactccag gaaataaaat atcatagtaa tcatctggcc tatacaaatc tacagccatt
     cctggttgtt gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta
     taccccactt gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca
     gctatagcag caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca
     gcaattgcct cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca
     attgcagcag cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca
     gcttcagaca cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg
     aaataaaaga caaaaagact aaacttacca gttaactttc tggtttttca gttaaccttt
     ctggtttttt gcgtttcccg tcaacagtat cttccccttc acaaaattgc agcaaaagct
     ctaaaacaaa cacaaaaagg cgttgagctg tttttttact ttcagtccat gacctacgaa
     ccttaacgga ggcctggcgt gacagccggc gcagcaccat ggcctgaaat aacctctgaa
     agaggaactt ggttaggtac cttctgaggc ggaaagaacc ag
//