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ID   PBR322TN5  preliminary; circular DNA; SYN; 12173 BP.
XX
AC   IG0024; J01749;
XX
DT   11-DEC-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBR322Tn5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR322Tn5 from pBR322 & Tn5
RA   ;
RT   ;
RL   Unpublished (1991).
XX
CC   NM (pBR322Tn5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp
FT                   2. Tn5 5818bp, neo gene/strep gene/bleo gene/#U00004
FT                   -> pBR322Tn5 12173bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3105..3965)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   repeat_unit     5368..6900
FT                   /note="RPT E. coli IR region, from Tn5"
FT   CDS             complement(7603..8401)
FT                   /note="ANT E. coli streptomycin resistance gene (str),
FT                   from Tn5"
FT   CDS             complement(8840..9636)
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo),
FT                   from Tn5"
FT   CDS             complement(8842..8820)
FT                   /note="ANT E. coli bleomycin gene (ble), from Tn5"
FT   repeat_unit     complement(9638..11185)
FT                   /note="RPT E. coli IR region, from Tn5"
XX
SQ   Sequence 12173 BP; 4349 A; 2927 C; 2802 G; 2095 T; 0 other;
     gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
     cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc
     tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
     cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
     atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat
     gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
     gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
     ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
     gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
     tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg
     gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg
     ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc
     catgaacaga aattccccct tacacggagg catcaagtga ccaaacagga aaaaaccgcc
     cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag
     ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt
     taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     cgaggccctt tcgtcttcaa gaattcaaaa aaaaaaaaaa aaaaagttga cccgagaaaa
     aatgatcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa atgatcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaatga ctcttataca caagtagcgt cctgaacgga acctttcccg
     ttttccagga tctgacttcc atgtgacctc ctaacatggt aacgttcatg ataacttctg
     ctcttcatcg tgcggccgac tgggctaaat ctgtgttctc ttcggcggcg ctgggtgatc
     ctcgccgtac tgcccgcttg gttaacgtcg ccgcccaatt ggcaaaatat tctggtaaat
     caataaccat ctcatcagag ggtagtgaag ccatgcagga aggcgcttac cgattttacc
     gcaatcccaa cgtttctgcc gaggcgatca gaaaggctgg cgccatgcaa acagtcaagt
     tggctcagga gtttcccgaa ctgctggcca ttgaggacac cacctctttg agttatcgcc
     accaggtcgc cgaagagctt ggcaagctgg gctctattca ggataaatcc cgcggatggt
     gggttcactc cgttctcttg ctcgaggcca ccacattccg caccgtagga ttactgcatc
     aggagtggtg gatgcgcccg gatgaccctg ccgatgcgga tgaaaaggag agtggcaaat
     ggctggcagc ggccgcaact agccggttac gcatgggcag catgatgagc aacgtgattg
     cggtctgtga ccgcgaagcc gatattcatg cttatctgca ggacaggctg gcgcataacg
     agcgcttcgt ggtgcgctcc aagcacccac gcaaggacgt agagtctggg ttgtatctga
     tcgaccatct gaagaaccaa ccggagttgg gtggctatca gatcagcatt ccgcaaaagg
     gcgtggtgga taaacgcggt aaacgtaaaa atcgaccagc ccgcaaggcg agcttgagcc
     tgcgcagtgg gcgcatcacg ctaaaacagg ggaatatcac gctcaacgcg gtgctggccg
     aggagattaa cccgcccaag ggtgagaccc cgttgaaatg gttgttgctg accggcgaac
     cggtcgagtc gctagcccaa gccttgcgcg tcatcgacat ttatacccat cgctggcgga
     tcgaggagtt ccataaggca tggaaaaccg gagcaggagc cgagaggcaa cgcatggagg
     agccggataa tctggagcgg atggtctcga tcctctcgtt tgttgcggtc aggctgttac
     agctcagaga aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag
     cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     gctggggcgc cctctgggaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg ggccggccca
     cgctgtcgtc caatctccca agacacgccg ccaccgcgca ccgtcgcggc gagctgctcc
     ccaagccgct gttcgatggg cttccaccgc acgaggctgg accccttggc gtcatcaagc
     atcccatttc gcccgctggc gagcatgacg gggcgccggt agacgccggc aacgcgctgg
     ccgtcggcca cggggcgatg ctccaggccg gtatcggcgg caatgtcctt cgcggcctgc
     gccagttccc gagcccgctg ctgcccagca gattccggtg aggatcaccg cgctgcccgc
     gccgctcggc cagtccctgt tcggccagga agtccgcgcg ctgctgtatc gcctacttgg
     cctcactgct aaagcccagg tcgcccaagc ccgagccacc gtcgatcaac tgctggtcaa
     gccaggtggc accgatcacg cgggcctgcc gctccatggg caggtgcgat ttcagctcca
     ccgtcacgcc accaagacgc tgggcgtcat agcggcggcc ctgctcgggc agatcgtccg
     gcaccttcca tagtccctcg gccacgcaca ccacgatgcc ggcctggtgc agggcttcga
     ggcggcgggt gtggcccgca acgacttcca gcggatcacg cccgacacgg cctgacctag
     ctcgacgacc aggtgagatc ggtgaccgcg ctagtcaagc aaccggcgtg ccatggcgtt
     tacggccaga tcaatcgcag cgccctcgcc ctcgccgtcg ccgtcgccga tgaaccaggc
     tgccgacaag cccgtccatg caatgatcca gggaagaagc cggttcgggc tcaaacccgg
     tcgtcgcgac cacaatgctg agtcgagcct ccagcctgcc cggcaggatc gcaagcgggc
     gaccggggtc gctgagatcg ggattcgtga agatgttggc atagtcgaag gtgcgctcgc
     cgagcagtcc gtgcgggtcg atggccagcc agccgcggtc gccgaagtcg agcacgttct
     cgtggtgcag gtcgccgtgg agcgggcaca cctcgcgcgg cgccgccaga agttggcgcg
     ctacgctggc ggcgggcgca agtgccgcgt gctcagcggc caaccggaaa agcggctgga
     accattcctg tagcggatgg agatcgggcg gcggtccgga ccgcggcgcg tgcagacgag
     cggcggtgtc gcagaggatc ctgcaagcct cgtcgtcctg gccggaccac gctatctgtg
     caaggtcccc ggccccggac gcgcgctcca tgagcagagc gcccgccgcc gaggcgaaga
     gtcggggggc gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg
     gaatgcgggc gaccttcagc atcgccggca tgtccccctg gcggacgcga agtatccagc
     tcgagtgggt ggtgagcagc tcgccgtcgc gcagcaggcg ccagcgctcc atccacgcag
     tgacctcagg cggaaaacgg gaagacacac tcatgagatg cctgcaagca attcgttctg
     tatcaggcgc aggagcgtcc cgtccgggtc gaccaaagcg gccatcgtgc ctccccactc
     ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
     tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
     cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     gcgcttgcgt tttcccttgt ccagatagcc cagtagtgac attcatccgg ggtcagcacc
     gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt
     gcttgcggca gcgtgaagct ttctctgagc tgtaacagcc tgaccgcaac aaacgagagg
     atcgagacca tccgctccag attatccggc tcctccatgc gttgcctctc ggctcctgct
     ccggttttcc atgccttatg gaactcctcg atccgccagc gatgggtata aatgtcgatg
     acgcgcaagg cttgggctag cgactcgacc ggttcgccgg tcagcaacaa ccatttcaac
     ggggtctcac ccttgggcgg gttaatctcc tcggccagca ccgcgttgag cgtgatattc
     ccctgtttta gcgtgatgcg cccactgcgc aggctcaagc tcgccttgcg ggctggtcga
     tttttacgtt taccgcgttt atccaccacg cccttttgcg gaatgctgat ctgatagcca
     cccaactccg gttggttctt cagatggtcg atcagataca acccagactc tacgtccttg
     cgtgggtgct tggagcgcac cacgaagcgc tcgttatgcg ccagcctgtc ctgcagataa
     gcatgaatat cggcttcgcg gtcacagacc gcaatcacgt tgctcatcat gctgcccatg
     cgtaaccggc tagttgcggc cgctgccagc catttgccac tctccttttc atccgcatcg
     gcagggtcat ccgggcgcat ccaccactcc tgatgcagta atcctacggt gcggaatgtg
     gtggcctcga gcaagagaac ggagtgaacc caccatccgc gggatttatc ctgaatagag
     cccagcttgc caagctcttc ggcgacctgg tggcgataac tcaaagaggt ggtgtcctca
     atggccagca gttcgggaaa ctcctgagcc aacttgactg tttgcatggc gccagccttt
     ctgatcgcct cggcagaaac gttgggattg cggtaaaatc ggtaagcgcc ttcctgcatg
     gcttcactac cctctgatga gatggttatt gatttaccag aatattttgc caattgggcg
     gcgacgttaa ccaagcgggc agtacggcga ggatcaccca gcgccgccga agagaacaca
     gatttagccc agtcggccgc acgatgaaga gcagaagtta tcatgaacgt taccatgtta
     ggaggtcaca tggaagtcag atcctggaaa acgggaaagg ttccgttcag gacgctactt
     gtgtataaga gtcaggttaa acaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagat atc
//