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ID   PBR322TN9  preliminary; circular DNA; SYN; 5134 BP.
XX
AC   IG9951;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBR322-Tn9 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSV2 from pBR322 & SV40
RC   pSV2-cat from pSV2
RC   pRSVneo, pRSVcat, pRSVgpt, pRSV/betaglobin from RSV
RA   Gorman C.M.;
RT   "High efficiency transfer into mammalian cells";
RL   DNA Cloning 2:143-190(1985).
RL   Glover D.M., ed., IRL Press, Oxford.
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pBR322-Tn9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 ClaI 4361bp 25..25
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pBR322-Tn9 5134bp"
FT   -               1..24
FT                   /note="pBR322 1..24 24bp
FT                   ClaI = AT^CGAT
FT                   TaqI =  T^CGA"
FT   -               25..797
FT                   /note="Tn9 215..987 773bp
FT                   TaqI =  T^CGA
FT                   ClaI = AT^CGAT"
FT   -               798..5134
FT                   /note="pBR322 25..4361 4337bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5134 BP; 1194 A; 1372 C; 1314 G; 1254 T; 0 other;
     ttctcatgtt tgacagctta tcatcgagat tttcaggagc taaggaagct aaaatggaga
     aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg
     gatgaatggc agaaattcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa
     cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg
     agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
     ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
     tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
     cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggccct ttcgtcttca agaa
//