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ID PBR325 preliminary; circular DNA; SYN; 5996 BP.
XX
AC L08855; VB0002; IG0025; J01749; J01750; J01751; J01752;
XX
DT 11-DEC-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pBR325 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pBR325 from pBR322 & phage P1Cm, Tn9 cat gene
RA Prentki P., Karch F., Iida S., Meyer J.;
RT "The plasmid cloning vector pBR325 contains a 483 base-pair-long
RT inverted duplication";
RL Gene 14:289-299(1981).
XX
RN [2]
RC pBR324 from pBR322 & pMB1, structural genes/immunity genes
RC pBR325 from pBR322 & phage P1Cm, Tn9 cat gene
RA Bolivar F.;
RT "Construction and characterization of new cloning vehicles III.
RT Derivatives of plasmid pBR322 carrying unique EcoRI sites for
RT selection of EcoRI generated recombinant DNA molecules";
RL Gene 4:121-136(1978).
XX
RN [3]
RC pBR325 from pBR322
RC pBR327 from pBR322
RC pBR327-par from pBR327 & pSC101
RC pBR328 from pBR327 & pBR325
RC pBR329 from pBR327 & pBR328
RC ColE1, pMB1, ColDF13, RSF1030, p15A from ColE1-like plasmids
RA Balbas P., Soberon X., Merino E., Zurita M., Lomeli H., Valle F.,
RA Flores N., Bolivar F.;
RT "Plasmid vector pBR322 and its special-purpose derivatives - a
RT review";
RL Gene 50:3-40(1986).
XX
RN [4]
RP 4358-5444
RC pDV801 from pBR322 & phage P1Cm, Tn9 cat gene
RC pDV802, pDV803 from pDV801
RC [M13::Tn3-15 from M13 & Tn3]
RC M13pbla6 from M13::Tn3-15
RC M13pCmA, M13pCmB from M13pbla6 & phage P1Cm, Tn9 cat gene
RA Alton N.K., Vapnek D.;
RT "Nucleotide sequence analysis of the chloramphenicol resistance
RT transposon Tn9";
RL Nature 282:864-869(1979).
XX
RN [5]
RP 1-5996
RC pBR325
RA Gilbert W.;
RT "VecBase 3.0";
RL Unpublished (1991).
XX
CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Revised 16-DEC-1986 by F. Pfeiffer:
CC There is a sequence conflict in that pMAC58 contains a TTG
CC sequence where
CC its parent pBR325 at pos 5264-5265 only contains a TG. It is
CC unclear, if
CC this is a mutation in pMAC58, or if the sequence of pBR325 is
CC incorrect at that position.
CC The plasmid pBR325 is a cloning vector constructed in vitro by
CC the addition of the chloramphenicol resistance gene of an
CC IS1-flanked transposon to pBR322. Sequence analysis suggests
CC that this transposon is related to transposons Tn9 and Tn981
CC but is longer. No portion of insertion sequence IS1 was found
CC in the genome.
CC This sequence was reconstructed from the genome of pBR322,
CC transposons Tn9 and Tn981, and pBR325 genome fragments. The
CC actual pBR325 and pBR328 genomes may differ from this
CC sequence.
CC In pMAC58, a pBR325-derived vector, a TTG sequence was found
CC where pBR325 has only a TG (pos 5264-65).
CC It is not known if this discrepancy is a
CC mutation or a sequence error in pBR325.
CC
CC Size is 5927 bp. for Amersham.
CC Size is 5996 bp. for BRL.
CC NM (pBR325)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Amersham)(BRL)(Sigma)
CC HO (E.coli HB101)
CC CP ()
CC FN (cloning general)
CC SE ()
CC PA (pBR322)
CC BR (pBR327)(pBR328)
CC OF (pBR328)(pBR329)(pJSC73)(pMAC5-8)(pMAC5bg14)(pSV2cat)(YRp17)
CC OF (pAA3.7X)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4353bp, [5..4358]
FT 2. Tn9 1086bp, cat gene/#V00622 1142bp [4358..5444]
FT 3. Tn981 76bp, [5444..5520]
FT 3. pBR322 481bp, 3' tet invert dup [5520..5996..5]
FT -> pBR325 5996bp [EcoRI in cat]"
FT misc_binding 23..23
FT /note="SIT unique ClaI"
FT promoter complement(27..33)
FT /note="PRO E. coli promoter P1"
FT misc_binding 29..29
FT /note="SIT unique HindIII"
FT CDS 86..1273
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT promoter 43..49
FT /note="PRO E. coli promoter P2"
FT misc_binding 185..185
FT /note="SIT unique EcoRV"
FT misc_binding 229..229
FT /note="SIT unique NheI"
FT misc_binding 375..375
FT /note="SIT unique BamHI"
FT misc_binding 409..409
FT /note="SIT unique SgrAI"
FT misc_binding 562..562
FT /note="SIT unique SphI"
FT misc_binding 622..622
FT /note="SIT unique EcoNI"
FT misc_binding 651..651
FT /note="SIT unique SalI"
FT misc_binding 1425..1425
FT /note="SIT unique AvaI"
FT misc_binding 1668..1668
FT /note="SIT unique MamI"
FT CDS 1917..2105
FT /note="REP E. coli ROP gene"
FT misc_binding 2227..2227
FT /note="SIT double BsaAI"
FT misc_binding 2246..2246
FT /note="SIT unique Bst1107I"
FT misc_binding complement(2271..2271)
FT /note="SIT cAMP-dependent pBR-P4 transcription
FT initiation site"
FT misc_binding 2297..2297
FT /note="SIT unique NdeI"
FT misc_binding 2353..2353
FT /note="SIT double EarI"
FT misc_binding 2475..2475
FT /note="SIT unique AflIII"
FT rep_origin complement(2535..2535)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(3293..4156)
FT /note="ANT E. coli beta-lactamase gene (bla E-286)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3539..3539
FT /note="SIT unique AseI"
FT misc_binding 3609..3609
FT /note="SIT unique PstI"
FT misc_binding 3735..3735
FT /note="SIT unique PvuI"
FT misc_binding 3884..3884
FT /note="SIT unique BcgI"
FT CDS complement(3298..4155)
FT /note="GEN E. coli penicillinase gene (EC 3.5.2.6),
FT precursor"
FT sig_peptide complement(4155..4087)
FT /note="E. coli penicillinase gene signal peptide"
FT misc_binding 4157..4157
FT /note="SIT double EarI"
FT promoter complement(4188..4194)
FT /note="PRO E. coli promoter P3"
FT misc_binding 4286..4286
FT /note="SIT unique AatII"
FT misc_binding 4356..4356
FT /note="SIT double NspV"
FT CDS 4566..5222
FT /note="ANT E. coli chloramphenicol acetyltransferase
FT gene (CAT) chloramphenicol resistance gene (cmr/cml)
FT EC 2.3.1.28"
FT misc_binding 4470..4470
FT /note="SIT double BsaAI"
FT misc_binding 4779..4779
FT /note="SIT unique EcoRI"
FT misc_binding 5080..5080
FT /note="SIT unique NcoI"
FT misc_binding 5308..5308
FT /note="SIT double NspV"
FT misc_binding 5383..5383
FT /note="SIT unique AgeI"
FT misc_binding 5483..5483
FT /note="SIT unique BclI"
FT misc_binding 5763..5763
FT /note="SIT unique BspMI"
FT misc_feature complement(1..5)
FT /note="from pBR322"
FT misc_feature 5..4358
FT /note="from pBR322"
FT misc_feature 4358..5263
FT /note="from Tn981"
FT misc_feature 4358..5444
FT /note="from Tn9"
FT misc_feature complement(5520..5996)
FT /note="from pBR322"
XX
SQ Sequence 5996 BP; 1395 A; 1600 C; 1565 G; 1436 T; 0 other;
aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc
gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg
gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgac
//