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ID   PBR325     preliminary; circular DNA; SYN; 5996 BP.
XX
AC   L08855; VB0002; IG0025; J01749; J01750; J01751; J01752;
XX
DT   11-DEC-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pBR325 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR325 from pBR322 & phage P1Cm, Tn9 cat gene
RA   Prentki P., Karch F., Iida S., Meyer J.;
RT   "The plasmid cloning vector pBR325 contains a 483 base-pair-long
RT   inverted duplication";
RL   Gene 14:289-299(1981).
XX
RN   [2]
RC   pBR324 from pBR322 & pMB1, structural genes/immunity genes
RC   pBR325 from pBR322 & phage P1Cm, Tn9 cat gene
RA   Bolivar F.;
RT   "Construction and characterization of new cloning vehicles III.
RT   Derivatives of plasmid pBR322 carrying unique EcoRI sites for
RT   selection of EcoRI generated recombinant DNA molecules";
RL   Gene 4:121-136(1978).
XX
RN   [3]
RC   pBR325 from pBR322
RC   pBR327 from pBR322
RC   pBR327-par from pBR327 & pSC101
RC   pBR328 from pBR327 & pBR325
RC   pBR329 from pBR327 & pBR328
RC   ColE1, pMB1, ColDF13, RSF1030, p15A from ColE1-like plasmids
RA   Balbas P., Soberon X., Merino E., Zurita M., Lomeli H., Valle F.,
RA   Flores N., Bolivar F.;
RT   "Plasmid vector pBR322 and its special-purpose derivatives - a
RT   review";
RL   Gene 50:3-40(1986).
XX
RN   [4]
RP   4358-5444
RC   pDV801 from pBR322 & phage P1Cm, Tn9 cat gene
RC   pDV802, pDV803 from pDV801
RC   [M13::Tn3-15 from M13 & Tn3]
RC   M13pbla6 from M13::Tn3-15
RC   M13pCmA, M13pCmB from M13pbla6 & phage P1Cm, Tn9 cat gene
RA   Alton N.K., Vapnek D.;
RT   "Nucleotide sequence analysis of the chloramphenicol resistance
RT   transposon Tn9";
RL   Nature 282:864-869(1979).
XX
RN   [5]
RP   1-5996
RC   pBR325
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised 16-DEC-1986 by F. Pfeiffer:
CC   There is a sequence conflict in that pMAC58 contains a TTG
CC   sequence where
CC   its parent pBR325 at pos 5264-5265 only contains a TG. It is
CC   unclear, if
CC   this is a mutation in pMAC58, or if the sequence of pBR325 is
CC   incorrect at that position.
CC   The plasmid pBR325 is a cloning vector constructed in vitro by
CC   the addition of the chloramphenicol resistance gene of an
CC   IS1-flanked transposon to pBR322. Sequence analysis suggests
CC   that this transposon is related to transposons Tn9 and Tn981
CC   but is longer. No portion of insertion sequence IS1 was found
CC   in the genome.
CC   This sequence was reconstructed from the genome of pBR322,
CC   transposons Tn9 and Tn981, and pBR325 genome fragments. The
CC   actual pBR325 and pBR328 genomes may differ from this
CC   sequence.
CC   In pMAC58, a pBR325-derived vector, a TTG sequence was found
CC   where pBR325 has only a TG (pos 5264-65).
CC   It is not known if this discrepancy is a
CC   mutation or a sequence error in pBR325.
CC
CC   Size is 5927 bp. for Amersham.
CC   Size is 5996 bp. for BRL.
CC   NM (pBR325)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Amersham)(BRL)(Sigma)
CC   HO (E.coli HB101)
CC   CP ()
CC   FN (cloning general)
CC   SE ()
CC   PA (pBR322)
CC   BR (pBR327)(pBR328)
CC   OF (pBR328)(pBR329)(pJSC73)(pMAC5-8)(pMAC5bg14)(pSV2cat)(YRp17)
CC   OF (pAA3.7X)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4353bp, [5..4358]
FT                   2. Tn9 1086bp, cat gene/#V00622 1142bp [4358..5444]
FT                   3. Tn981 76bp, [5444..5520]
FT                   3. pBR322 481bp, 3' tet invert dup [5520..5996..5]
FT                   -> pBR325 5996bp [EcoRI in cat]"
FT   misc_binding    23..23
FT                   /note="SIT unique ClaI"
FT   promoter        complement(27..33)
FT                   /note="PRO E. coli promoter P1"
FT   misc_binding    29..29
FT                   /note="SIT unique HindIII"
FT   CDS             86..1273
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   promoter        43..49
FT                   /note="PRO E. coli promoter P2"
FT   misc_binding    185..185
FT                   /note="SIT unique EcoRV"
FT   misc_binding    229..229
FT                   /note="SIT unique NheI"
FT   misc_binding    375..375
FT                   /note="SIT unique BamHI"
FT   misc_binding    409..409
FT                   /note="SIT unique SgrAI"
FT   misc_binding    562..562
FT                   /note="SIT unique SphI"
FT   misc_binding    622..622
FT                   /note="SIT unique EcoNI"
FT   misc_binding    651..651
FT                   /note="SIT unique SalI"
FT   misc_binding    1425..1425
FT                   /note="SIT unique AvaI"
FT   misc_binding    1668..1668
FT                   /note="SIT unique MamI"
FT   CDS             1917..2105
FT                   /note="REP E. coli ROP gene"
FT   misc_binding    2227..2227
FT                   /note="SIT double BsaAI"
FT   misc_binding    2246..2246
FT                   /note="SIT unique Bst1107I"
FT   misc_binding    complement(2271..2271)
FT                   /note="SIT cAMP-dependent pBR-P4 transcription
FT                   initiation site"
FT   misc_binding    2297..2297
FT                   /note="SIT unique NdeI"
FT   misc_binding    2353..2353
FT                   /note="SIT double EarI"
FT   misc_binding    2475..2475
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(2535..2535)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3293..4156)
FT                   /note="ANT E. coli beta-lactamase gene (bla E-286)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3539..3539
FT                   /note="SIT unique AseI"
FT   misc_binding    3609..3609
FT                   /note="SIT unique PstI"
FT   misc_binding    3735..3735
FT                   /note="SIT unique PvuI"
FT   misc_binding    3884..3884
FT                   /note="SIT unique BcgI"
FT   CDS             complement(3298..4155)
FT                   /note="GEN E. coli penicillinase gene (EC 3.5.2.6),
FT                   precursor"
FT   sig_peptide     complement(4155..4087)
FT                   /note="E. coli penicillinase gene signal peptide"
FT   misc_binding    4157..4157
FT                   /note="SIT double EarI"
FT   promoter        complement(4188..4194)
FT                   /note="PRO E. coli promoter P3"
FT   misc_binding    4286..4286
FT                   /note="SIT unique AatII"
FT   misc_binding    4356..4356
FT                   /note="SIT double NspV"
FT   CDS             4566..5222
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (cmr/cml)
FT                   EC 2.3.1.28"
FT   misc_binding    4470..4470
FT                   /note="SIT double BsaAI"
FT   misc_binding    4779..4779
FT                   /note="SIT unique EcoRI"
FT   misc_binding    5080..5080
FT                   /note="SIT unique NcoI"
FT   misc_binding    5308..5308
FT                   /note="SIT double NspV"
FT   misc_binding    5383..5383
FT                   /note="SIT unique AgeI"
FT   misc_binding    5483..5483
FT                   /note="SIT unique BclI"
FT   misc_binding    5763..5763
FT                   /note="SIT unique BspMI"
FT   misc_feature    complement(1..5)
FT                   /note="from pBR322"
FT   misc_feature    5..4358
FT                   /note="from pBR322"
FT   misc_feature    4358..5263
FT                   /note="from Tn981"
FT   misc_feature    4358..5444
FT                   /note="from Tn9"
FT   misc_feature    complement(5520..5996)
FT                   /note="from pBR322"
XX
SQ   Sequence 5996 BP; 1395 A; 1600 C; 1565 G; 1436 T; 0 other;
     aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
     ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
     atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
     tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
     gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
     ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc
     gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg
     gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
     tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgac
//