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ID   PBR327     preliminary; circular DNA; SYN; 3273 BP.
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AC   V00083; J02549; ATCC37516;
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DT   03-NOV-1982 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBR327 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3273
RC   pBR327 from pBR322
RC   pBR328 from pBR327 & pBR325
RA   Soberon X., Covarrubias L., Bolivar F.;
RT   "Construction and characterization of new cloning vehicles. IV.
RT   Deletion derivatives of pBR322 and pBR325";
RL   Gene 9:287-305(1980).
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RN   [2]
RC   pBR327 from pBR322
RA   Lederberg E.M.;
RT   ;
RL   Recombinant DNA Technical Bulletin NIH 2:0-0(1980).
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RN   [3]
RC   pBR325 from pBR322
RC   pBR327 from pBR322
RC   pBR327-par from pBR327 & pSC101
RC   pBR328 from pBR327 & pBR325
RC   pBR329 from pBR327 & pBR328
RC   ColE1, pMB1, ColDF13, RSF1030, p15A from ColE1-like plasmids
RA   Balbas P., Soberon X., Merino E., Zurita M., Lomeli H., Valle F.,
RA   Flores N., Bolivar F.;
RT   "Plasmid vector pBR322 and its special-purpose derivatives - a
RT   review";
RL   Gene 50:3-40(1986).
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CC   pBR327 (chimeric) was not resequenced but deduced from the
CC   pBR322 sequence.
CC   Derived from pBR322 by deleting sequences between nt 1430 (AvaI)
CC   and 2519, thus removing the bom or Mob site.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBR327)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli GM21)(E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR (pBR328)(pBR329)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove AvaI-? 1088bp 1426..2514,
FT                   \ bom/Mob site 3273bp
FT                   -> pBR327 3273bp"
FT   CDS             259..1275
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             complement(2205..3068)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   misc_binding    0..0
FT                   /note="SIT unique AvaI"
FT   misc_binding    0..0
FT                   /note="SIT unique ClaI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HgiEII"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NruI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SphI"
FT   misc_binding    0..0
FT                   /note="SIT unique XmaIII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
XX
SQ   Sequence 3273 BP; 721 A; 911 C; 835 G; 806 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggcccgt ggccggggga
     ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaa
//