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ID   PBR328     preliminary; circular DNA; SYN; 4907 BP.
XX
AC   L08858; VB0004; IG0307; ATCC37517; V00083; J02549;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pBR328 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-1431; 2521-4356
RC   pBR327 from pBR322
RC   pBR328 from pBR327 & pBR325
RA   Soberon X., Covarrubias L., Bolivar F.;
RT   "Construction and characterization of new cloning vehicles. IV.
RT   Deletion derivatives of pBR322 and pBR325";
RL   Gene 9:287-305(1980).
XX
RN   [2]
RC   pBR325 from pBR322
RC   pBR327 from pBR322
RC   pBR327-par from pBR327 & pSC101
RC   pBR328 from pBR327 & pBR325
RC   pBR329 from pBR327 & pBR328
RC   ColE1, pMB1, ColDF13, RSF1030, p15A from ColE1-like plasmids
RA   Balbas P., Soberon X., Merino E., Zurita M., Lomeli H., Valle F.,
RA   Flores N., Bolivar F.;
RT   "Plasmid vector pBR322 and its special-purpose derivatives - a
RT   review";
RL   Gene 50:3-40(1986).
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RN   [3]
RP   1-5996
RC   pBR328
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pBR325, Fragment 'TTCT',5-1431,2521-5996
CC   pBR328 is a deletion derivative of pBR325.
CC   Contains a 482 bp inverted duplication.
CC   Constructed from the PstI/BamHI fragment of pBR327 containing the
CC   replication origin and the PstI/BamHI fragment of pBR325 containing
CC   the cml gene.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBR328)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Boehringer)(ATCC)
CC   HO (E.coli RR1)(E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning general)
CC   SE ()
CC   PA (pBR322)(pBR325)
CC   BR (pBR327)
CC   OF (pBR329)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR327 BamHI-PstI 2148bp 376..2524,cat/EcoRI
FT                   2. pBR325 PstI-BamHI 2758bp 3612..5994..376, AvaI
FT                   -> pBR328 4907bp"
FT   misc_binding    23..23
FT                   /note="SIT unique ClaI"
FT   misc_binding    29..29
FT                   /note="SIT unique HindIII"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    185..185
FT                   /note="SIT unique EcoRV"
FT   misc_binding    229..229
FT                   /note="SIT unique NheI"
FT   misc_binding    375..375
FT                   /note="SIT unique BamHI"
FT   misc_binding    562..562
FT                   /note="SIT unique SphI"
FT   misc_binding    651..651
FT                   /note="SIT unique SalI"
FT   misc_binding    1425..1425
FT                   /note="SIT unique AvaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2209..2997)
FT                   /note="ANT E. coli beta-lactamase gene (bla E-286)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2452..2452
FT                   /note="SIT unique AsnI"
FT   misc_binding    2524..2524
FT                   /note="SIT unique PstI"
FT   misc_binding    2649..2649
FT                   /note="SIT unique PvuI"
FT   misc_binding    2878..2878
FT                   /note="SIT unique Asp700I"
FT   misc_binding    3075..3075
FT                   /note="SIT unique Ksp6321"
FT   misc_binding    3201..3201
FT                   /note="SIT unique AatII"
FT   misc_binding    3383..3383
FT                   /note="SIT unique BsaAI"
FT   CDS             3477..4136
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    3590..3590
FT                   /note="SIT unique PvuII"
FT   misc_binding    3690..3690
FT                   /note="SIT unique EcoRI"
FT   misc_binding    3957..3957
FT                   /note="SIT unique BalI"
FT   misc_binding    3991..3991
FT                   /note="SIT unique NcoI"
FT   misc_binding    4237..4237
FT                   /note="SIT unique AspI"
FT   misc_binding    4344..4344
FT                   /note="SIT unique SauI"
FT   misc_binding    4394..4394
FT                   /note="SIT unique BclI"
FT   misc_feature    1..1431
FT                   /note="from pBR322"
FT   misc_feature    1432..4907
FT                   /note="from pBR325"
FT   misc_feature    complement(4431..4907)
FT                   /note="from pBR322"
XX
SQ   Sequence 4907 BP; 1131 A; 1302 C; 1264 G; 1210 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg ccgcgttgct
     ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggccct ttcgtcttcg aataaatacc tgtgacggaa gatcacttcg
     cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
     gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa gtgataataa
     gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt
     cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt
     gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta
     ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac ggtgaatccg
     ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta
     ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgac
//